Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	10,865	A→C	G164G (GGT→GGG)	msyB ←	acidic protein MsyB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	10,865	0	A	C	100.0%	86.5 / NA	25	G164G (GGT→GGG)	msyB	acidic protein MsyB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (13/12);  total (13/12)

TTTAGTGCCGCACCTACAGGGCCTCCCAGCCCCGCGCCGCGCAGCAAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACACCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATTTTCTTCCAGG  >  NZ_CP009273/10743‑10983                                                                                                                           |                                                                                                                       tttAGTGCCGCACCTACAGGGCCTCCCAGCCCCGCGCCGCGCAGCAAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAAtt                                                                                                        >  2:7004/1‑139 (MQ=255)     gTGCCGCACCTACAGGGCCTCCCAGCCCCGCGCCGCGCAGCAAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGt                                                                                                     <  1:340438/138‑1 (MQ=255)                        tCCCAGCCCCGCGCCGCGCAGCAAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGgcag                                                                                  >  2:57085/1‑138 (MQ=255)                         cccAGCCCCGCGCCGCGCAGCAAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGgcag                                                                                  >  2:492393/1‑137 (MQ=255)                                   cgccgcgcAGCAAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCt                                                                                                   <  2:473492/110‑1 (MQ=255)                                   cgccgcgcAGCAAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCt                                                                                                   >  1:473492/1‑110 (MQ=255)                                         gcagcaAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCAtt                                                                <  2:77753/139‑1 (MQ=255)                                                  cATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTgg                                                        >  2:164930/1‑138 (MQ=255)                                                     gCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATc                                                    >  1:178104/1‑139 (MQ=255)                                                                cTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCa                                         <  2:9349/139‑1 (MQ=255)                                                                     tgctgcGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAAc                                    >  2:13783/1‑139 (MQ=255)                                                                          cgcgggTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCAtt                                                                >  2:177368/4‑106 (MQ=255)                                                                          cGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCAtt                                                                <  1:177368/106‑1 (MQ=255)                                                                          cGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTcc                                 <  1:419320/137‑1 (MQ=255)                                                                                        aTGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGt                 <  1:411297/139‑1 (MQ=255)                                                                                         tGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTc                >  2:186633/1‑139 (MQ=255)                                                                                             ggTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCAtt            <  1:73010/139‑1 (MQ=255)                                                                                             ggTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCAtt            >  2:335954/1‑139 (MQ=255)                                                                                              gTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAAtt                                                                                                        >  2:320609/1‑46 (MQ=255)                                                                                              gTCAGTTGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAAtt                                                                                                        <  1:320609/46‑1 (MQ=255)                                                                                                   ttGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGa                                       >  2:373654/1‑106 (MQ=255)                                                                                                   ttGGCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGa                                       <  1:373654/106‑1 (MQ=255)                                                                                                     ggCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATTTTCTTcc     <  1:335954/138‑1 (MQ=255)                                                                                                     ggCTGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATTTTCTTcc     <  1:298980/138‑1 (MQ=255)                                                                                                        tGGAAAGCAAATGCGACACCCCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATTTTCTTCCAgg  >  1:131768/1‑138 (MQ=255)                                                                                                                           |                                                                                                                       TTTAGTGCCGCACCTACAGGGCCTCCCAGCCCCGCGCCGCGCAGCAAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACACCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCGTGCAGAAACTCCTGCTTATGTTCCTCGTCCATTTTCTTCCAGG  >  NZ_CP009273/10743‑10983

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑