Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	232,843	T→C	I97T (ATC→ACC)	dnaQ →	DNA polymerase III subunit epsilon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	232,843	0	T	C	100.0%	62.1 / NA	17	I97T (ATC→ACC)	dnaQ	DNA polymerase III subunit epsilon
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/9);  total (8/9)

ACCCGATCGGCTGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTA  >  NZ_CP009273/232712‑232974                                                                                                                                    |                                                                                                                                    aCCCGATCGGCTGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAAc                                                                                                                              >  1:70633/1‑139 (MQ=255)               tggATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGatat                                                                                                                <  2:425946/139‑1 (MQ=255)                                        tGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCg                                                                                       >  1:55540/1‑139 (MQ=255)                                               gCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCtt                                                                                 >  1:108878/1‑138 (MQ=255)                                                   aTGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTa                                                                                                 >  2:7767/1‑118 (MQ=255)                                                   aTGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTa                                                                                                 <  1:7767/118‑1 (MQ=255)                                                     gAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAgcgc                                                                           >  1:356169/1‑138 (MQ=255)                                                                        ccACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGTCCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATAc                                                       <  1:335628/139‑1 (MQ=255)                                                                                    aaGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGt                                           <  1:394419/139‑1 (MQ=255)                                                                                    aaGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGt                                           <  2:43804/139‑1 (MQ=255)                                                                                        aGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTa                                               <  2:80383/131‑1 (MQ=255)                                                                                        aGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTa                                               >  1:80383/1‑131 (MQ=255)                                                                                          ccGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGa                                     <  2:458503/139‑1 (MQ=255)                                                                                          ccGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGa                                     <  2:460137/139‑1 (MQ=255)                                                                                                 gTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCtt                              >  1:390607/1‑139 (MQ=255)                                                                                                            atatTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAg                   >  1:129413/1‑139 (MQ=255)                                                                                                                             ttGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTa  <  2:233906/139‑1 (MQ=255)                                                                                                                                    |                                                                                                                                    ACCCGATCGGCTGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGTA  >  NZ_CP009273/232712‑232974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑