Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,263,514	T→C	intergenic (+553/+61)	yhaC → / ← rnpB	YhaC family protein/RNase P RNA component class A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,263,514	0	T	C	100.0%	68.5 / NA	21	intergenic (+553/+61)	yhaC/rnpB	YhaC family protein/RNase P RNA component class A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/9);  total (12/9)

CAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGC  >  NZ_CP009273/3263398‑3263646                                                                                                                     |                                                                                                                                     cAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGCCATTCATCTTTCTGCCCCTCCa                                                                                                                <  1:362245/139‑1 (MQ=37)              ttCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGCCATTCATCTTTCTGCCCCTCCAAAAGCAAAAAccc                                                                                                   >  2:476069/1‑139 (MQ=37)                                 aaacCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCTCTTTCTTTTGGGTATAGCGTCGTGGACAGCCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACg                                                                                <  1:211245/139‑1 (MQ=255)                                         ccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGCCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAg                                                                        >  1:412994/1‑139 (MQ=37)                                         ccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGCCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGGTTTTACGTAAATCAg                                                                        >  2:295232/1‑139 (MQ=255)                                                                   gTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGCCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTc                                              >  2:341352/1‑139 (MQ=255)                                                                       aaCTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGCCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTc                                          <  2:62001/139‑1 (MQ=255)                                                                            aCCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGCCATTCATCTTTCTGCCCCTCCACAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGcc                                                    >  1:459588/1‑124 (MQ=25)                                                                            aCCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGCCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGAGAAGcc                                                    <  2:459588/124‑1 (MQ=25)                                                                             ccGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGCCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGa                                     >  1:124923/1‑138 (MQ=255)                                                                             ccGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGCCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGAc                                    <  1:252198/139‑1 (MQ=255)                                                                                 tAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGCCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGtc                                >  2:56637/1‑139 (MQ=255)                                                                                 tAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGCCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGt                                 >  1:44756/1‑138 (MQ=255)                                                                                   aGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGCCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGtcat                              <  1:107399/139‑1 (MQ=255)                                                                                           tctttTGGGTATAGCGTCGTGGACAGCCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATc                                                                          >  1:476967/1‑87 (MQ=37)                                                                                           tctttTGGGTATAGCGTCGTGGACAGCCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATc                                                                          <  2:476967/87‑1 (MQ=37)                                                                                                  ggTATAGCGTCGTGGACAGCCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGc                >  1:364527/1‑138 (MQ=255)                                                                                                       aGCGTCGTGGACAGCCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGc          >  2:109950/1‑139 (MQ=255)                                                                                                       aGCGTCGTGGACAGCCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGc          >  1:215753/1‑139 (MQ=255)                                                                                                          gtcgtGGACAGCCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCa       <  2:215753/139‑1 (MQ=255)                                                                                                               ggAAAGCCATTCAACTTACGGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGc  <  2:99580/139‑1 (MQ=21)                                                                                                                     |                                                                                                                                     CAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGC  >  NZ_CP009273/3263398‑3263646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑