| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 3,332,009 | A→G | V200A (GTC→GCC) | mlaE ← | lipid asymmetry maintenance ABC transporter permease subunit MlaE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,332,009 | 0 | A | G | 100.0% | 78.1 / NA | 21 | V200A (GTC→GCC) | mlaE | lipid asymmetry maintenance ABC transporter permease subunit MlaE |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (10/11); total (10/11) | |||||||||||
GCCAGAGACGAGTGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGCCCCAGATCCCCACGGCGACGAAAA > NZ_CP009273/3331874‑3332126 | gCCAGAGACGAGTGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGGCCa < 2:1155/139‑1 (MQ=255) gTGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGGCCAGATCCATACGc > 1:10158/1‑139 (MQ=255) gTGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGGCCAGATCCATACGc > 2:177019/1‑139 (MQ=255) aCGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGGCCAGATCCATACGCCAGTCGa < 2:10158/139‑1 (MQ=255) gTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGGCCAGATCCATACGCCAGTCGACGGCATTTTGCa > 1:372725/1‑139 (MQ=255) ccGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGGCCAGATCCATACGCCAGTCGACGGCATTTTGCATTGcc < 1:282843/139‑1 (MQ=255) cGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGGCCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAgaagaa < 1:353806/139‑1 (MQ=255) cAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGGCCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAAccc > 2:487524/1‑139 (MQ=255) gTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGGCCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTAt < 2:178451/139‑1 (MQ=255) cgtcgtAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGGCCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCttt > 2:189216/1‑139 (MQ=255) ttAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGGCCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGc < 2:336225/139‑1 (MQ=255) aaaTCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGGCCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCag < 2:42528/95‑1 (MQ=255) aaaTCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGGCCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCag > 1:42528/1‑95 (MQ=255) aTCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGGCCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATc < 2:266118/139‑1 (MQ=255) cACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGGCCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCg < 1:487524/138‑1 (MQ=255) aTGGCGAACACCACGCTCTTAATCAGACAGTTGGCCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGCCCCAGATCCCCa > 2:382647/1‑139 (MQ=255) cGCTCTTAATCAGACAGTTGGCCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGCCCCAGATCCCCACGGCGACGaaaa > 1:135918/1‑138 (MQ=255) ttGGCCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCg > 1:61224/1‑57 (MQ=255) ttGGCCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCg < 2:61224/57‑1 (MQ=255) ttGGCCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATc > 2:365756/1‑94 (MQ=255) ttGGCCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATc < 1:365756/94‑1 (MQ=255) | GCCAGAGACGAGTGGACAACGGTGCGAGTGGTTGCCCGGCTAATCCCGGCAGACGTCGGGATGGCGTCGTAGCCGTTAAACAACGAAATCCACGTCACCGTGATGGCGAACACCACGCTCTTAATCAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGCCCCAGATCCCCACGGCGACGAAAA > NZ_CP009273/3331874‑3332126 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |