Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,473,413	A→G	M185T (ATG→ACG)	kefB ←	glutathione‑regulated potassium‑efflux system protein KefB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,473,413	0	A	G	100.0%	138.6 / NA	38	M185T (ATG→ACG)	kefB	glutathione‑regulated potassium‑efflux system protein KefB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/23);  total (15/23)

AATGCGGAACCCAACACCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCATGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATTC  >  NZ_CP009273/3473278‑3473547                                                                                                                                        |                                                                                                                                       aaTGCGGAACCCAACACCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGc                                                                                                                                     <  2:213381/139‑1 (MQ=255)   tGCGGAACCCAACACCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGcc                                                                                                                                    >  2:45874/1‑138 (MQ=255)   tGCGGAACCCAACACCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGcc                                                                                                                                    >  2:390794/1‑138 (MQ=255)   tGCGGAACCCAACACCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGcc                                                                                                                                    >  2:104004/1‑138 (MQ=255)   tGCGGAACCCAACACCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGcag                                                                                                                                   >  2:244015/1‑137 (MQ=255)     cGGAACCCAACACCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGAc                                                                                                                                 <  1:244015/139‑1 (MQ=255)                acCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGt                                                                                                                      <  2:52453/139‑1 (MQ=255)                  cagcagcagCGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCg                                                                                                                    <  1:390794/139‑1 (MQ=255)                            gTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGt                                                                                                          >  2:22110/1‑139 (MQ=255)                                                 cGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACg                                                                                     <  2:113895/139‑1 (MQ=255)                                                   cACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGc                                                                                   <  1:22110/139‑1 (MQ=255)                                                      gCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACt                                                                                <  2:275061/139‑1 (MQ=255)                                                           aaGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCCCCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGc                                                                           <  1:284166/139‑1 (MQ=255)                                                           aaGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGc                                                                           <  1:326783/139‑1 (MQ=255)                                                                gCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACt                                                                                >  1:14179/1‑129 (MQ=255)                                                                gCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACt                                                                                <  2:14179/129‑1 (MQ=255)                                                                        gCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGAt                                                               >  1:323495/1‑138 (MQ=255)                                                                               aCCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCa                                                       <  2:423438/139‑1 (MQ=255)                                                                               aCCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCa                                                       <  2:374485/139‑1 (MQ=255)                                                                                ccGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCa                                                       <  1:45874/138‑1 (MQ=255)                                                                                 cGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGa                                                     <  1:65639/139‑1 (MQ=255)                                                                                            aaTAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAAcag                                          <  1:353158/139‑1 (MQ=255)                                                                                               aGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCa                                                       <  2:252775/123‑1 (MQ=255)                                                                                               aGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCa                                                       >  1:252775/1‑123 (MQ=255)                                                                                                  gcCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGcc                                                                                                                                    >  1:26464/1‑43 (MQ=255)                                                                                                  gcCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCGTGcc                                                                                                                                    <  2:26464/43‑1 (MQ=255)                                                                                                    ccaccaATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGcc                                                                                            >  1:297014/1‑81 (MQ=255)                                                                                                    ccaccaATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGcc                                                                                            <  2:297014/81‑1 (MQ=255)                                                                                                    ccaccaATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGc                                                                           <  1:41459/98‑1 (MQ=255)                                                                                                    ccaccaATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGc                                                                           >  2:41459/1‑98 (MQ=255)                                                                                                      accaATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGa                                 >  1:344040/1‑138 (MQ=255)                                                                                                      accaATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGa                                 >  1:476266/1‑138 (MQ=255)                                                                                                      accaATCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGa                                 >  2:472877/1‑138 (MQ=255)                                                                                                           tCAGCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGAAAAccc                           >  2:457744/1‑139 (MQ=255)                                                                                                              gCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAg                        <  2:251211/139‑1 (MQ=255)                                                                                                              gCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAg                        <  2:323495/139‑1 (MQ=255)                                                                                                              gCATGCCGACAAACGCCAGCACCTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAg                        <  1:472877/139‑1 (MQ=255)                                                                                                                                    cTTCGTGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATTc  <  1:209162/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       AATGCGGAACCCAACACCAGCAGCAGCGTCGCGGCGGTGAACACTTCCCGCACGCCAGAAGCTGCAATAAAGCGGAATACCGGACGCAGTAAATAGCGCCCACCAATCAGCATGCCGACAAACGCCAGCACCTTCATGCCGACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATTC  >  NZ_CP009273/3473278‑3473547

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑