Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,639,274	T→C	V177A (GTC→GCC)	rlmJ →	23S rRNA (adenine(2030)‑N(6))‑methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,639,274	0	T	C	100.0%	69.3 / NA	20	V177A (GTC→GCC)	rlmJ	23S rRNA (adenine(2030)‑N(6))‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/13);  total (7/13)

CGACGGTTTCCAGCAGCTTAAGGCC‑AAA‑C‑TGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGATCTGGAAGCGACCGGTAT  >  NZ_CP009273/3639170‑3639391                                                                                                            |                                                                                                                      cGACGGTTTCCAGCAGCTTAAGGCC‑AAA‑C‑TGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGc                                                                                      >  2:41924/1‑138 (MQ=255)              cagcTTAAGGCC‑AAA‑C‑TGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACg                                                                        <  1:41924/139‑1 (MQ=255)               cagctAAGGCCAAAA‑C‑TGCCGCGGTTTCCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACg                                                                        <  2:202849/135‑1 (MQ=255)               agtttAAGACC‑AAC‑C‑GGCCGCCGTTTCCCCGCCGTGGTTTAATCCTTAGCGCCCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGc                                                                       <  1:18998/136‑1 (MQ=255)                 gctAAAGCC‑AAACC‑GGCCGCGGTTTCCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCa                                                                      <  2:28457/137‑1 (MQ=255)                 cTTAAGGCC‑AAA‑C‑TGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCAc                                                                     >  1:360863/1‑139 (MQ=255)                 actAAAGCC‑AAA‑CTTGCCGCGGTTTCCCCGCCGTGGTTTAATCCTTACCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCa                                                                      <  2:115202/137‑1 (MQ=255)                    aaGGCC‑AAC‑C‑TCCCGCCGTTTCCCCGCCGGGGTGTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACtg                                                                   <  2:46655/138‑1 (MQ=255)                    aaGGCC‑AAA‑C‑TGCCGCCGGTTTCCCGCCGTGTTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACtg                                                                   <  1:442231/138‑1 (MQ=255)                            aa‑C‑TGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATcc                                                           >  2:71849/1‑139 (MQ=255)                            aa‑C‑TGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGAGGTATcc                                                           >  2:342606/1‑139 (MQ=255)                                              ccgccgTGTTTTAACCCTTACCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAg                                           <  2:296642/139‑1 (MQ=255)                                                           aTCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCa                              <  2:421083/139‑1 (MQ=255)                                                           aTCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCa                              <  2:70714/139‑1 (MQ=255)                                                                tATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATc                         >  2:210039/1‑139 (MQ=255)                                                                  tCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCa                       <  1:435025/139‑1 (MQ=255)                                                                  tCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCa                       <  1:342606/139‑1 (MQ=255)                                                                      cccGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGAt                   >  1:143881/1‑139 (MQ=255)                                                                                  aatgaaaACTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGATCTGGAAGCGAcc       >  2:319743/1‑139 (MQ=255)                                                                                       aaaCTGACTATCAAGCGGTGGCCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGATCTGGAAGCGACCGGTAt  <  2:126103/139‑1 (MQ=255)                                                                                                            |                                                                                                                      CGACGGTTTCCAGCAGCTTAAGGCC‑AAA‑C‑TGCCGCCGGTTTCCCGCCGTGGTTTAATCCTTATCGACCCGCCGTATGAAATGAAAACTGACTATCAAGCGGTGGTCAGCGGGATAGCAGAAGGTTACAAACGTTTCGCCACTGGTATTTACGCACTGTGGTATCCGGTGGTGCTGCGTCAGCAAATTAAGCGCATGATCCACGATCTGGAAGCGACCGGTAT  >  NZ_CP009273/3639170‑3639391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑