Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,747,593	C→G	A66P (GCC→CCC)	yiaW ←	DUF3302 domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,747,593	0	C	G	100.0%	171.0 / NA	46	A66P (GCC→CCC)	yiaW	DUF3302 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (22/24);  total (22/24)

CATAAGTATTACTCGGCAGAAGAGGATTTGATGTCGGCGAGCTGTTTCTCCAGCCCGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGCCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACG‑TTTTTTGGCAATCAGGTAGGGAATATCATGTAAAATAATGA  >  NZ_CP009273/3747457‑3747724                                                                                                                                         |                                                                                                                                     cATAAGTATTACTCGGCAGAAGAGGATTTGATGTCGGCGAGCTGTTTCTCCAGCCCGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGgcc                                                                                                                                    <  1:197958/139‑1 (MQ=255)   tAAGTATTACTCGGCAGAAGAGGATTTGATGTCGGCGAGCTGTTTCTCCAGCCCGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCa                                                                                                                                   >  1:342298/1‑138 (MQ=255)     aGTATTACTCGGCAGAAGAGGATTTGATGTCGGCGAGCTGTTTCTCCAGCCCGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAAt                                                                                                                                >  2:264489/1‑139 (MQ=255)          tACTCGGCAGAAGAGGATTTGATGTCGGCGAGCTGTTTCTCCAGCCCGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCcac                                                                                                                            >  1:250204/1‑138 (MQ=255)                 cAGAAGAGGATTTGATGTCGGCGAGCTGTTTCTCCAGCCCGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACgg                                                                                                                    >  2:268248/1‑139 (MQ=255)                 cAGAAGAGGATTTGATGTCGGCGAGCTGTTTCTCCAGCCCGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACgg                                                                                                                    >  2:148224/1‑139 (MQ=255)                  agaagaGGATTTGATGTCGGCGAGCTGTTTCTCCAGCCCGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGc                                                                                                                   <  1:264489/139‑1 (MQ=255)                   gaagaGGATTTGATGTCGGCGAGCTGTTTCTCCAGCCCGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGcc                                                                                                                  <  1:268248/139‑1 (MQ=255)                   gaagaGGATTTGATGTCGGCGAGCTGTTTCTCCAGCCCGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGcc                                                                                                                  <  2:239161/139‑1 (MQ=255)                       agGATTTGATGTCGGCGAGCTGTTTCTCCAGCCCGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGAt                                                                                                              <  1:21500/139‑1 (MQ=255)                        ggATTTGATGTCGGCGAGCTGTTTCTCCAGCCCGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATa                                                                                                             <  2:342298/139‑1 (MQ=255)                           tttGATGTCGGCGAGCTGTTTCTCCAGCCCGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCaa                                                                                                                                  <  2:148321/115‑1 (MQ=255)                           tttGATGTCGGCGAGCTGTTTCTCCAGCCCGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCaa                                                                                                                                  >  1:148321/1‑115 (MQ=255)                               aTGTCGGCGAGCTGTTTCTCCAGCCCGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGt                                                                                                       <  1:154530/138‑1 (MQ=255)                                tGTCGGCTAGCTGTTTCTCCAGCCCGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTaa                                                                                                     <  1:180195/139‑1 (MQ=255)                                   cGGCGAGCTGTTTCTCCAGCCCGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGa                                                                                                               <  1:248483/126‑1 (MQ=255)                                   cGGCGAGCTGTTTCTCCAGCCCGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGa                                                                                                               >  2:248483/1‑126 (MQ=255)                                    ggCGAGCTGTTTCTCCAGCCCGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGt                                                                                                  >  1:478599/1‑138 (MQ=255)                                                        cGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGc                                                                             <  2:478599/139‑1 (MQ=255)                                                        cGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGc                                                                             <  1:148224/139‑1 (MQ=255)                                                                cgcTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGaa                                                                     <  1:82804/139‑1 (MQ=255)                                                                 gcTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAAt                                                                    <  2:250204/139‑1 (MQ=255)                                                                               aTAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAg                                                      >  1:394316/1‑139 (MQ=255)                                                                               aTAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAg                                                      >  1:455375/1‑139 (MQ=255)                                                                               aTAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAg                                                      >  2:170650/1‑139 (MQ=255)                                                                                        gAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTAc                                             >  1:91399/1‑139 (MQ=255)                                                                                              tGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACG‑ttttt                                      >  1:166055/1‑139 (MQ=255)                                                                                                    tGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGaa                                                                     <  1:118486/103‑1 (MQ=255)                                                                                                    tGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGaa                                                                     >  2:118486/1‑103 (MQ=255)                                                                                                    tGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACG‑tttttttggc                                 >  1:186813/1‑134 (MQ=255)                                                                                                      cATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACG‑TTTTTTGGCAAt                               <  2:394316/138‑1 (MQ=255)                                                                                                       aTTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACGTTTTTTTGGCAATc                              <  2:473343/139‑1 (MQ=255)                                                                                                       aTTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACGTTTTTTTGGCAATc                              <  2:192242/139‑1 (MQ=255)                                                                                                        ttCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACG‑TTTTTTGGCAATCAg                            <  1:402636/139‑1 (MQ=255)                                                                                                           cccATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTa                                                                                                      >  1:157611/1‑63 (MQ=255)                                                                                                           cccATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTa                                                                                                      <  2:157611/63‑1 (MQ=255)                                                                                                           cccATCCCCGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACG‑TTTTTTGGCAATCAgg                           >  2:414250/1‑137 (MQ=255)                                                                                                                   cGCTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACGTTTTTTTGGCAATCAGGTAGGGAata                  >  1:38492/1‑139 (MQ=255)                                                                                                                     cTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCAc                                                                                      >  2:403685/1‑69 (MQ=255)                                                                                                                     cTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCAc                                                                                      <  1:403685/69‑1 (MQ=255)                                                                                                                     cTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACGTTTTTTTGGCAATCAGGTAGGGAATATc                <  2:226615/139‑1 (MQ=255)                                                                                                                     cTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACG‑TTTTTTGGCAATCAGGTAGGGAATATCa               >  2:154255/1‑139 (MQ=255)                                                                                                                     cTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACG‑TTTTTTGGCAATCAGGTAGGGAATATCa               >  1:119225/1‑139 (MQ=255)                                                                                                                     cTCCGGGCGGTAAAGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACG‑TTTTTTGGCAATCAGGTAGGGAATATCa               >  2:369513/1‑139 (MQ=255)                                                                                                                                 aaGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACGTTTTTTTGGCAATCAGGTAGGGAATATCATGTAAaataat    <  2:38492/139‑1 (MQ=255)                                                                                                                                 aaGCGTGGGCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACG‑TTTTTTGGCAATCAGGTAGGGAATATCATGTAAaataat    <  1:154255/138‑1 (MQ=255)                                                                                                                                  aGCGTGCGCCAAATCCACAAAAACGGCAAGATAACATGTAACGTAAAAAGGCTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACG‑TTTTTTGGCAATCAGGTAGGGAATATCATGTAAAATAATGa  <  1:211817/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     CATAAGTATTACTCGGCAGAAGAGGATTTGATGTCGGCGAGCTGTTTCTCCAGCCCGGCAATGCGCTGTTGCAGTTGCATAACGGATGAATCATGGCTTTGCATTCCCCATCCCCGCTCCGGGCGGTAAAGCGTGGCCCAAATCCACAAAAACGGCCAGATAACATGTAACGTAAAAAGACTCACCCAACCAGCAACATGAATGGCGTCGGCATGAGGATGATTACG‑TTTTTTGGCAATCAGGTAGGGAATATCATGTAAAATAATGA  >  NZ_CP009273/3747457‑3747724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑