Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,876,284	T→C	D269G (GAT→GGT)	dnaA ←	chromosomal replication initiator protein DnaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,876,284	0	T	C	100.0%	130.4 / NA	36	D269G (GAT→GGT)	dnaA	chromosomal replication initiator protein DnaA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/17);  total (19/17)

TCGTTTTCGTCGGCCTTTTTCATCAGGATCGCCACACGGGTTTCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGATCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATCAGCAGTGCATCTACGGAACGGTAGTAGC  >  NZ_CP009273/3876149‑3876419                                                                                                                                        |                                                                                                                                        tCGTTTTCGTCGGCCTTTTTCATCAGGATCGCCACACGGGTTTCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGAccc                                                                                                                                       <  1:61687/138‑1 (MQ=255) tCGTTTTCGTCGGCCTTGTTCATCAGGATCGCCACACGGGTTTCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGAccc                                                                                                                                       <  1:198602/138‑1 (MQ=255)    ttttCGTCGGCCTTTTTCATCAGGATCGCCACACGGGTTTCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAgg                                                                                                                                   <  2:285010/139‑1 (MQ=255)    ttttCGTCGGCCTTTTTCATCAGGATCGCCACACGGGTTTCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAgg                                                                                                                                   >  1:285010/1‑139 (MQ=255)              ccTTTTTCATCAGGATCGCCACACGGGTTTCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGAt                                                                                                                         <  1:269896/139‑1 (MQ=255)                        caGGATCGCCACACGGGTTTCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTa                                                                                                               >  2:230842/1‑139 (MQ=255)                         aGGATCGCCACACGGGTTTCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTa                                                                                                               <  2:12759/138‑1 (MQ=255)                           gATCGCCACACGGGTTTCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCt                                                                                                            >  2:231366/1‑139 (MQ=255)                                   cacGGGTTTCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCcagcag                                                                                                    <  1:377394/139‑1 (MQ=255)                                         tttCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGtt                                                                                              >  1:293521/1‑139 (MQ=255)                                           tCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTg                                                                                             >  2:192038/1‑138 (MQ=255)                                                        gTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGaaaaa                                                                               <  2:419777/139‑1 (MQ=255)                                                                gCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCt                                                                       <  1:230842/139‑1 (MQ=255)                                                                               cAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCcagcag                                                                                                    >  2:447072/1‑95 (MQ=255)                                                                               cAACCGAAGCGGGAATTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTCCCTTCcagcag                                                                                                    <  1:447072/95‑1 (MQ=255)                                                                                aaCCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGtt                                                                                                                    >  1:393808/1‑78 (MQ=255)                                                                                aaCCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGtt                                                                                                                    <  2:393808/78‑1 (MQ=255)                                                                                     aaGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCa                                                   >  1:87848/1‑138 (MQ=255)                                                                                             tttCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGa                                          <  2:360932/139‑1 (MQ=255)                                                                                                     gATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCcagcag                                                                                                    >  2:254182/1‑73 (MQ=255)                                                                                                     gATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCcagcag                                                                                                    <  1:254182/73‑1 (MQ=255)                                                                                                     gATCCTCAACGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATAtcgtc                                  >  1:110296/1‑139 (MQ=255)                                                                                                            aaCGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATcagc                           <  2:169164/139‑1 (MQ=255)                                                                                                              cGCCGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATcagcag                         <  1:192038/139‑1 (MQ=255)                                                                                                                ccGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATCAGCAGTg                       <  2:192667/139‑1 (MQ=255)                                                                                                                ccGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATCAGCAGTg                       <  1:198670/139‑1 (MQ=255)                                                                                                                 cGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATCAGCAGTg                       >  2:198670/1‑138 (MQ=255)                                                                                                                 cGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATCAGCAGTg                       >  1:389346/1‑138 (MQ=255)                                                                                                                 cGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATCAGCAGTg                       >  1:364879/1‑138 (MQ=255)                                                                                                                 cGTTGATCTCTTTCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATCAGCAGTg                       >  2:363383/1‑138 (MQ=255)                                                                                                                           tttCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATCAGCAGTGc                      <  1:347967/129‑1 (MQ=255)                                                                                                                           tttCGGATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATCAGCAGTGc                      >  2:347967/1‑129 (MQ=255)                                                                                                                                gATAGCGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATCAGCAGTGCATCTACGGAACgg         >  1:134237/1‑137 (MQ=255)                                                                                                                                     cGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAatat                                      >  1:52189/1‑103 (MQ=255)                                                                                                                                     cGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAatat                                      <  2:52189/103‑1 (MQ=255)                                                                                                                                     cGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATCAGCAGTg                       <  2:401816/118‑1 (MQ=255)                                                                                                                                     cGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATCAGCAGTg                       >  1:401816/1‑118 (MQ=255)                                                                                                                                     cGACCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATCAGCAGTGCATCTACGGAACGGTAGTAGc  >  2:306338/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                        TCGTTTTCGTCGGCCTTTTTCATCAGGATCGCCACACGGGTTTCCAGCTCTGGCGGTTCGATCGCCACAGTCAGTCCCCAACCGAAGCGGGATTTCAAACGATCCTCAACGCCGTTGATCTCTTTCGGATAGCGATCCGAGGTGAGAATGATCTGTTGATTACCTTCCAGCAGGGCGTTGAAGGTGTGGAAAAACTCTTCCTGAGATCGTTCTTTATTAGCAAAAAACTGAATATCGTCGATCAGCAGTGCATCTACGGAACGGTAGTAGC  >  NZ_CP009273/3876149‑3876419

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑