Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,062,410	G→T	P76Q (CCG→CAG)	yihQ ←	sulfoquinovosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,062,410	0	G	T	100.0%	85.4 / NA	22	P76Q (CCG→CAG)	yihQ	sulfoquinovosidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (11/11);  total (11/11)

GCGCAGCCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATC  >  NZ_CP009273/4062277‑4062534                                                                                                                                      |                                                                                                                             gcgcAGCCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCTGTGAc                                                                                                                         <  2:427818/139‑1 (MQ=255)                     ggTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCgg                                                                                                      >  1:114977/1‑138 (MQ=255)                      gTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGt                                                                                                     >  1:250037/1‑138 (MQ=255)                      gTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGt                                                                                                     <  1:443261/138‑1 (MQ=255)                           ggTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAgcg                                                                                                >  2:409064/1‑138 (MQ=255)                                tCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAAttt                                                                                          >  1:5920/1‑139 (MQ=255)                                tCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAAttt                                                                                          >  2:169414/1‑139 (MQ=255)                                tCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAAtt                                                                                           <  1:409064/138‑1 (MQ=255)                                  gTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAAtttt                                                                                         <  2:458788/138‑1 (MQ=255)                                        tAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGt                                                                                  <  2:114977/139‑1 (MQ=255)                                                           cccTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTg                                                                >  2:310040/1‑138 (MQ=255)                                                                                ttCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGa                                          >  2:206245/1‑139 (MQ=255)                                                                                  cAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGat                                         <  2:5920/138‑1 (MQ=255)                                                                                   aGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGata                                        <  1:410018/138‑1 (MQ=255)                                                                                       tGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGata                                        >  2:159515/1‑134 (MQ=255)                                                                                       tGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGata                                        <  1:159515/134‑1 (MQ=255)                                                                                       tGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGc                                   >  2:383477/1‑139 (MQ=255)                                                                                               aTGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATAcc                             <  2:259743/137‑1 (MQ=255)                                                                                                                      aaTTAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTa    <  1:459797/139‑1 (MQ=255)                                                                                                                        ttAACCAACCATCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATc  >  2:377952/1‑139 (MQ=255)                                                                                                                                ccaTCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACcgcg                                                     >  2:376573/1‑80 (MQ=255)                                                                                                                                ccaTCCTGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACcgcg                                                     <  1:376573/80‑1 (MQ=255)                                                                                                                                      |                                                                                                                             GCGCAGCCAGATACGGTTGTGGTTAAGGTTGTCGTTTTGTAGTTCCAGCAATAAACGCCCCTGATCGTCGGCAGAGATATTCAGCGTGGCGCTAATGTCAGAACCACGGCTGAAATGAATTAACCAACCATCCGGTGACTGGCTGACGATGGCGTCGGTAAGCGCAATTTTCTCCTGTAGTTTATCTTTAATGCTGAAATTACCGCGGAACATATCGATATCCGCTATACCTGAGCCAATCCATAAACAAGGATTATC  >  NZ_CP009273/4062277‑4062534

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑