Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,091,145	C→T	G136G (GGC→GGT)	sodA →	superoxide dismutase [Mn]

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,091,145	0	C	T	100.0%	127.4 / NA	33	G136G (GGC→GGT)	sodA	superoxide dismutase [Mn]
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/18);  total (15/18)

CCTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGTGGGAACATGCTTACTACCTGAAATTCCAGAACCG  >  NZ_CP009273/4091019‑4091279                                                                                                                               |                                                                                                                                       ccTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAATGTGATAAACTGGCg                                                                                                                            <  1:206539/139‑1 (MQ=255) ccTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGTGATAAACTGGCg                                                                                                                            <  1:111877/139‑1 (MQ=255)     cAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGTGATAAACTGGCggtg                                                                                                                         <  2:387701/138‑1 (MQ=255)     cAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGTGATAAACTGGCg                                                                                                                            <  2:332436/135‑1 (MQ=255)     cAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGTGATAAACTGGCg                                                                                                                            >  1:332436/1‑135 (MQ=255)          tGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGTGATAAACTGGCGGTGGTTTc                                                                                                                    >  1:457011/1‑138 (MQ=255)          tGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGTGATAAACTGGCGGTGGTTTc                                                                                                                    >  2:214735/1‑138 (MQ=255)          tGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGTGATAAACTGGCGGTGGTTTc                                                                                                                    >  2:226984/1‑138 (MQ=255)          tGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGTGATAAACTGGCGGTGGGTTc                                                                                                                    >  1:278112/1‑138 (MQ=255)                               aCGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGTGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCg                                                                                              <  1:311290/139‑1 (MQ=255)                               aCGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGTGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCg                                                                                              <  1:311291/139‑1 (MQ=255)                                         ggCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGTGATAAACTGGCGGTGGGTTCTACTGCTAACCAGGATTCTCCGCTGATGGGt                                                                                     >  2:85305/1‑138 (MQ=255)                                                     aaCTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGTGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGCTATTTCTg                                                                        >  1:162638/1‑139 (MQ=255)                                                                aGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGTGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGc                                                             <  1:115764/139‑1 (MQ=255)                                                                aGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGTGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGc                                                             <  1:48696/139‑1 (MQ=255)                                                                  aaTTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGTGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCtt                                                           >  2:328605/1‑139 (MQ=255)                                                                  aaTTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGTGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCtt                                                           >  1:61538/1‑139 (MQ=255)                                                                      tGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGTGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTccc                                                        <  1:226984/138‑1 (MQ=255)                                                                          aaaGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGTGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTa                                                   <  2:226176/139‑1 (MQ=255)                                                                           aaGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGTGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTAt                                                  <  1:10629/139‑1 (MQ=255)                                                                               ggCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGTGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGc                                              <  1:428972/139‑1 (MQ=255)                                                                                              tGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGTGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGTggg                                 <  1:214735/137‑1 (MQ=255)                                                                                                gTTCCGGCTGGGCATGGCTGGTGCTGAAAGGTGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGTGGGAAc                              <  2:483090/138‑1 (MQ=255)                                                                                                      gCTGGGCATGGCTGGTGCTGAAAGGTGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGTGGGAACATGCTta                       >  2:127264/1‑139 (MQ=255)                                                                                                              tGGCTGGTGCTGAAAGGTGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGTGGGAACATGCTTACTACCTGa               <  1:328605/139‑1 (MQ=255)                                                                                                                    gTGCTGAAAGGTGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGTGGGAACATGCTTACTACCTGAAATTcc         >  2:92758/1‑139 (MQ=255)                                                                                                                    gTGCTGAAAGGTGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGTGGGAACATGCTTACTACCTGAAATTc          >  1:488986/1‑138 (MQ=255)                                                                                                                    gTGCTGAAAGGTGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGTGGGAACATGCTTACTACCTGAAATTc          >  1:183498/1‑138 (MQ=255)                                                                                                                          aaaGGTGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGTGGGAACATGCTTACTACCTGAAATTCCAGAAcc   >  1:78827/1‑139 (MQ=255)                                                                                                                          aaaGGTGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGTGGGAACATGCTTACTACCTGAAATTCCAGAAcc   <  1:101037/139‑1 (MQ=255)                                                                                                                          aaaGGTGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGTGGGAACATGCTTACTACCTGAAATTCCAGAAcc   >  1:211820/1‑139 (MQ=255)                                                                                                                           aaGGTGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGTGGGAACATGCTTACTACCTGAAATTCCAGAAccg  <  2:278112/139‑1 (MQ=255)                                                                                                                           aaGGTGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGTGGGAACATGCTTACTACCTGAAATTCCAGAAccg  <  1:127264/139‑1 (MQ=255)                                                                                                                               |                                                                                                                                       CCTGCAGGGTGACCTGAAAGCGGCTATCGAACGTGACTTCGGCTCCGTTGATAACTTCAAAGCAGAATTTGAAAAAGCGGCAGCTTCCCGCTTTGGTTCCGGCTGGGCATGGCTGGTGCTGAAAGGCGATAAACTGGCGGTGGTTTCTACTGCTAACCAGGATTCTCCGCTGATGGGTGAAGCTATTTCTGGCGCTTCCGGCTTCCCGATTATGGGCCTGGATGTGTGGGAACATGCTTACTACCTGAAATTCCAGAACCG  >  NZ_CP009273/4091019‑4091279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑