Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,192,005	A→G	E51G (GAA→GGA)	zraS →	two‑component system sensor histidine kinase ZraS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,192,005	0	A	G	100.0%	130.7 / NA	34	E51G (GAA→GGA)	zraS	two‑component system sensor histidine kinase ZraS
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/20);  total (14/20)

TCCTTAGCTAAATGGTTAAGCGCGATCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGCCGGGAGTGTTGTGGTTCGCAGTCA  >  NZ_CP009273/4191881‑4192139                                                                                                                             |                                                                                                                                       tCCTTAGCTAAATGGTTAAGCGCGATCCTCCCCGTGGGCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAGGTAATGTGc                                                                                                                          >  1:109122/1‑139 (MQ=255)  ccTTAGCTAAATGGTTAAGCGCGATCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAGGTAATGTGCt                                                                                                                         >  2:113396/1‑139 (MQ=255)   cTTAGCTAAATGGTTAAGCGCGATCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAGGTAATGTGCt                                                                                                                         >  2:410701/1‑138 (MQ=255)        cTAAATGGTTAAGCGCGATCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAGGTAATGTGCTTATCcg                                                                                                                   <  2:288493/139‑1 (MQ=255)        cTAAATGGTTAAGCGCGATCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAGGTAATGTGCTTATCcg                                                                                                                   <  2:417135/139‑1 (MQ=255)        cTAAATGGTTAAGCGCGATCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAGGTAATGTGCTTATCcg                                                                                                                   <  2:421871/139‑1 (MQ=255)                     cgcgATCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAGGTAATGTGCTTATCcg                                                                                                                   >  1:44508/1‑126 (MQ=255)                     cgcgATCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAGGTAATGTGCTTATCcg                                                                                                                   <  2:44508/126‑1 (MQ=255)                        gATCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGa                                                                                                   <  1:113396/139‑1 (MQ=255)                         aTCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGaa                                                                                                  <  2:29244/139‑1 (MQ=255)                          tcctccCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAg                                                                                                 >  1:307958/1‑139 (MQ=255)                          tcctccCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAg                                                                                                 <  2:231157/139‑1 (MQ=255)                          tcctccCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAg                                                                                                 <  2:425502/139‑1 (MQ=255)                               cccGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCcgcg                                                                                            <  1:342004/139‑1 (MQ=255)                                    ggTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAg                                                                                                 >  1:194256/1‑129 (MQ=255)                                    ggTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAg                                                                                                 <  2:194256/129‑1 (MQ=255)                                    ggTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAgg                                                                                        >  1:78412/1‑138 (MQ=255)                                    ggTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAgg                                                                                        >  1:162199/1‑138 (MQ=255)                                    ggTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAgg                                                                                        >  2:270620/1‑138 (MQ=255)                                     gTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAggg                                                                                       <  1:114157/138‑1 (MQ=255)                                            ttgGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAgggatgggga                                                                                <  2:103267/138‑1 (MQ=255)                                              gggCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAgggatggggatg                                                                              <  2:162199/138‑1 (MQ=255)                                                    gtggGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAAt                                                                         <  1:270620/137‑1 (MQ=255)                                                    gtggGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATg                                                                        >  1:234158/1‑138 (MQ=255)                                                    gtggGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATg                                                                        >  1:97700/1‑138 (MQ=255)                                                    gtggGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATg                                                                        >  1:183566/1‑138 (MQ=255)                                                       ggATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCAcc                                                                    <  2:73143/139‑1 (MQ=255)                                                       ggATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCAcc                                                                    <  2:109122/139‑1 (MQ=255)                                                           tGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATg                                                                 >  1:434691/1‑138 (MQ=255)                                                                          tgATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGgcgc                                                  <  2:183566/138‑1 (MQ=255)                                                                          tgATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGgcgc                                                  <  1:443017/138‑1 (MQ=255)                                                                                         ggcgggcAAGCGAGGCAGACCGCCAGGCATTACTGGGAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATgg                                   <  1:410701/138‑1 (MQ=255)                                                                                                    gAGGCAGACCGCCAGGCATTACTGGGAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGCCgg                       <  2:258946/139‑1 (MQ=255)                                                                                                                         cTGGGAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGCCGGGAGTGTTGTGGTTCGCAGTCa  >  2:475090/1‑139 (MQ=255)                                                                                                                             |                                                                                                                                       TCCTTAGCTAAATGGTTAAGCGCGATCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGCCGGGAGTGTTGTGGTTCGCAGTCA  >  NZ_CP009273/4191881‑4192139

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑