Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	303,602	T→G	N481T (AAC→ACC)	ecpC ←	fimbrial usher EcpC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	303,602	0	T	G	100.0%	108.6 / NA	31	N481T (AAC→ACC)	ecpC	fimbrial usher EcpC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (18/13);  total (18/13)

TGTAGACATTTTGATAGTAATCTGCCGTGTAATAATGGCTGTTGTAACGGCGGTCATCATTGTAGCTGATGCTGAATGTGCCCAGCTTCGACCACAGTGAGTTCAGGTTGAGTGTGCCGCCGATTGCACGGTTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCAC  >  NZ_CP009273/303471‑303725                                                                                                                                    |                                                                                                                            tGTAGACATTTTGATAGTAATCTGCCGTGTAATAATGGCTGTTGTAACGGCGGTCATCATTTTAGCTGATGCTGGATTTGCCCAGCTTCGCCCCCCATGGGTTTAGGTTGTGTGTGCCCCCGATTTCCCGGGTGTCGGc                                                                                                                      >  1:59672/1‑139 (MQ=255)  gTAGACATTTTGATAGTAATCTGCCGTGTAATAATGGCTGTTGTAACGGCGGTCATCATTTTAGCTGGTGCTGAATGTGGCCCGCTTCGGCCACCGTGAGGTCAGGGTGAGGGGGCCGCCGGTTGCACGGGGGTCGGCa                                                                                                                     >  1:36820/1‑139 (MQ=255)          tttGATAGTAATCTGCCGTGTAATAATGGCTGTTGTAACGGCGGTCCACAATTTGGCTGATGCTGAATGTGGCCAGCGTCGAACACAGGGAGGTGTGGGTGTGTGTGGCGCCGGTTGCGCGGGGGTGGGCGTCGCTACg                                                                                                             >  1:65312/1‑139 (MQ=255)                                      gCTGTTGTAACGGCGGTCATCATTGTAGCTGATGCTGAATGTGCCCAGCTTCGACCACAGTGAGTTCAGGTTGAGTGTGCCGCCGATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGttttt                                                                                 <  1:402156/139‑1 (MQ=255)                                                    ggTCATCATTTTAGATGATGCTGAATGGGCCCAGGTTCGACCACAATGGGTTTAGGGTGGGGGGGCCGCCGGGTGTACGGGGGTCGGCCTCGCTACGGCGCAATTGATTGCCAATGCGGGGTTTTTCCTGGTTAACCca                                                                   >  2:145541/1‑139 (MQ=255)                                                                     aTGCTGAATGTGCCCAGCTTCGACCACAGTGAGTTCAGGTTGAGTGTGCCGCCGATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAg                                                                >  2:333146/1‑125 (MQ=255)                                                                     aTGCTGAATGTGCCCAGCTTCGACCACAGTGAGTTCAGGTTGAGTGTGCCGCCGATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAg                                                                >  1:316275/1‑125 (MQ=255)                                                                     aTGCTGAATGTGCCCAGCTTCGACCACAGTGAGTTCAGGTTGAGTGTGCCGCCGATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAg                                                                <  1:333146/125‑1 (MQ=255)                                                                     aTGCTGAATGTGCCCAGCTTCGACCACAGTGAGTTCAGGTTGAGTGTGCCGCCGATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAg                                                                <  2:316275/125‑1 (MQ=255)                                                                     aTGCTGAATGTGCCCAGCTTCGACCACAGTGAGTTCAGGTTGAGTGTGCCGCCGATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGcc                                                    <  2:85096/137‑1 (MQ=255)                                                                     aTGCTGAATGTGCCCAGCTTCGACCACAGTGAGTTCAGGTTGAGTGTGCCGCCGATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCt                                                   <  1:140934/138‑1 (MQ=255)                                                                     aTGCTGAATGTGCCCAGCTTCGACCACAGTGAGTTCAGGTTGAGTGTGCCGCCGATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTc                                                  >  1:408997/1‑139 (MQ=255)                                                                      tGCTGAATGTGCCCAGCTTCGACCACAGTGAGTTCAGGTTGAGTGTGCCGCCGATTGCACGGGTGTCGGCATCGc                                                                                                                 <  2:334974/75‑1 (MQ=255)                                                                      tGCTGAATGTGCCCAGCTTCGACCACAGTGAGTTCAGGTTGAGTGTGCCGCCGATTGCACGGGTGTCGGCATCGc                                                                                                                 >  1:334974/1‑75 (MQ=255)                                                                      tGCTGAATGTGCCCAGCTTCGACCACAGTGAGTTCAGGTTGAGTGTGCCGCCGATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTcc                                                 >  1:111817/1‑139 (MQ=255)                                                                           aaTGTGCCCAGCTTCGACCACAGTGAGTTCAGGTTGAGTGTGCCGCCGATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCgg                                              >  1:189510/1‑137 (MQ=255)                                                                               tgCCCAGCTTCGACCACAGTGAGTTCAGGTTGAGTGTGCCGCCGATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGt                                        >  2:484271/1‑139 (MQ=255)                                                                                     gCTTCGACCACAGTGAGTTCAGGTTGAGTGTGCCGCCGATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCt                                  >  1:80472/1‑139 (MQ=255)                                                                                     gCTTCGACCACAGTGAGTTCAGGTTGAGTGTGCCGCCGATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAAACCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCt                                  >  1:461023/1‑139 (MQ=255)                                                                                         cGACCACAGTGAGTTCAGGTTGAGTGTGCCGCCGATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGa                                <  1:410081/137‑1 (MQ=255)                                                                                           aCCACAGTGAGTTCAGGTTGAGTGTGCCGCCGATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGAGCTGAtgct                            >  1:116888/1‑139 (MQ=255)                                                                                             cacaGTGAGTTCAGGTTGAGTGTGCCGCCGATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGAtgctgc                          <  2:298541/139‑1 (MQ=255)                                                                                                  tGAGTTCAGGTTGAGTGTGCCGCCGATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGAtg                     >  1:142456/1‑139 (MQ=255)                                                                                                         aGGTTGAGTGTGCCGCCGATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTcc              <  1:484271/139‑1 (MQ=255)                                                                                                            ttGAGTGTGCCGCCGATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCa             <  1:369476/137‑1 (MQ=255)                                                                                                            ttGAGTGTGCCGCCGATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCa             >  2:369476/1‑137 (MQ=255)                                                                                                               aGTGGGCCGCCGATTGCACGGGTGTCGGCATCGCTCCGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGc        <  2:62034/139‑1 (MQ=255)                                                                                                                     ccgccgATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCa   <  1:285447/138‑1 (MQ=255)                                                                                                                     ccgccgATTGCACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCAc  >  1:181786/1‑139 (MQ=255)                                                                                                                               cACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTc                                                                               <  1:402249/52‑1 (MQ=255)                                                                                                                               cACGGGTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTc                                                                               >  2:402249/1‑52 (MQ=255)                                                                                                                                    |                                                                                                                            TGTAGACATTTTGATAGTAATCTGCCGTGTAATAATGGCTGTTGTAACGGCGGTCATCATTGTAGCTGATGCTGAATGTGCCCAGCTTCGACCACAGTGAGTTCAGGTTGAGTGTGCCGCCGATTGCACGGTTGTCGGCATCGCTACGTCGCAATTGATTGCCAATGCGGGTTTTTTCCTGATTAACCCACAGCGAACTAAAGCCTCCCGGTAGAGTGGCGCTGATGCTGCCGATGCTGCTCCATGAGCTGTCAC  >  NZ_CP009273/303471‑303725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑