Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,320,763	T→C	S149P (TCG→CCG)	proP →	glycine betaine/L‑proline transporter ProP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,320,763	0	T	C	100.0%	128.6 / NA	36	S149P (TCG→CCG)	proP	glycine betaine/L‑proline transporter ProP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/16);  total (20/16)

ATTATGTCGATCAGTACGTTCTGTATTGGCTTAATACCGTCCTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGTCGATATTTGTTGCGGAATACTCCCCTGACCGTAAACGTGGCTTTATGGGCAGCTGGCTGGACTTCGGTTCTATTGCCGGGTTTGTGCTGGGTGCGGGCGTGGT  >  NZ_CP009273/4320631‑4320866                                                                                                                                     |                                                                                                        aTTATGTCGATCAGTACGTTCTGTATTGGCTTAATACCGTCCTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGatat                                                                                                   >  1:213544/1‑139 (MQ=255) aTTATGTCGATCAGTACGTTCTGTATTGGCTTAATACCGTCCTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGatat                                                                                                   >  1:81558/1‑139 (MQ=255)    aTGTCGATCAGTACGTTCTGTATTGGCTTAATACCGTCCTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATttg                                                                                                >  1:241954/1‑139 (MQ=255)    aTGTCGATCAGTACGTTCTGTATTGGCTTAATACCGTCCTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATttg                                                                                                >  1:290607/1‑139 (MQ=255)    aTGTCGATCAGTACGTTCTGTATTGGCTTAATACCGTCCTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATttg                                                                                                >  1:45689/1‑139 (MQ=255)         gATCAGTACGTTCTGTATTGGCTTAATACCGTCCTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCg                                                                                           <  2:117687/139‑1 (MQ=255)         gATCAGTACGTTCTGTATTGGCTTAATACCGTCCTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCg                                                                                           <  1:55432/139‑1 (MQ=255)         gATCAGTACGTTCTGTATTGGCTTAATACCGTCCTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCg                                                                                           <  1:338863/139‑1 (MQ=255)              gTACGTTCTGTATTGGCTTAATACCGTCCTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATAGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATa                                                                                      <  1:270541/139‑1 (MQ=255)                    tCTGTATTGGCTTAATACCGTCCTACGACGCGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTcccc                                                                                >  2:246747/1‑139 (MQ=255)                      tGTATTGGCTTAATACCGTCCTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCt                                                                               >  1:178754/1‑138 (MQ=255)                        tATTGGCTTAATACCGTCCTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCt                                                                               <  2:213544/136‑1 (MQ=255)                        tATTGGCTTAATACCGTCCTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCt                                                                               <  2:241954/136‑1 (MQ=255)                        tATTGGCTTAATACCGTCCTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCt                                                                               <  1:332888/136‑1 (MQ=255)                                  aTACCGTCCTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGACCGTAAACGTg                                                                  <  2:191245/139‑1 (MQ=255)                                  aTACCGTCCTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGAACGTAAACGTg                                                                  >  2:446045/1‑139 (MQ=255)                                       gTCCTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTccc                                                                                 <  1:39107/119‑1 (MQ=255)                                       gTCCTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTccc                                                                                 >  2:39107/1‑119 (MQ=255)                                       gTCCTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGACCGTAAACGTGGCtt                                                              >  1:461354/1‑138 (MQ=255)                                       gTCCTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGACCGTAAACGTGGCtt                                                              >  2:229142/1‑138 (MQ=255)                                        tCCTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGACCGTAAACGTGGCTTTa                                                            >  2:331374/1‑139 (MQ=255)                                                  cGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGACCGTAAACGTGGCTTTATGGGCAgctg                                                  >  2:355237/1‑139 (MQ=255)                                                                   ccGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGACCGTAAACGTGGCTTTATGGGCAGCTGGCTGGACTTCGGTTCTa                                 >  1:359302/1‑139 (MQ=255)                                                                           gctgTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGACCGTAAACGTGGCTTTATGGGCAGCTGGCTGGACTTCGGTTCTATTGCCggg                         >  2:453702/1‑139 (MQ=255)                                                                                    cTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATttg                                                                                                >  2:87934/1‑59 (MQ=255)                                                                                    cTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATttg                                                                                                <  1:87934/59‑1 (MQ=255)                                                                                      gTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGACCGTAAACGTGGCTTTATGGGCAGCTGGCTGGACTTCGGTTCTATTGc                             <  2:173257/124‑1 (MQ=255)                                                                                      gTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGACCGTAAACGTGGCTTTATGGGCAGCTGGCTGGACTTCGGTTCTATTGc                             >  1:173257/1‑124 (MQ=255)                                                                                       tAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGACCGTAAACGTGGCTTTATGGGCAGCTGGCTGGACTTCGGTTCTATTGCCGGGTTTGTGCt                 <  1:246747/135‑1 (MQ=255)                                                                                       tAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGACCGTAAACGTGGCTTTATGGGCAGCTGGCTGGACTTCGGTTCTATTGCCGGGTTTGTGCt                 <  2:482813/135‑1 (MQ=255)                                                                                         aGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGACCGTAAACGTGGCTTTATGGGCAGCTGGCTGGACTTCGGTTCTATTGCCGGGTTTGTGCTGGGTGc           <  1:256204/139‑1 (MQ=255)                                                                                             ggCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGACCGTAAACGTGGCTTTATGGGCAGCTGGCTGGACTTCGGTTCTATTGCCGGGTTTGTGCTGGGTGCGGGc       >  1:204518/1‑139 (MQ=255)                                                                                               cacaAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGACCGTAAACGTGGCTTTATGGGCAGCTGGCTGGACTTCGGTTCTATTGCCGGGTTTGTGCTGGGTg            >  2:131355/1‑132 (MQ=255)                                                                                               cacaAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGACCGTAAACGTGGCTTTATGGGCAGCTGGCTGGACTTCGGTTCTATTGCCGGGTTTGTGCTGGGTg            <  1:131355/132‑1 (MQ=255)                                                                                                                            aCCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGACCGTAAACGTGGCTTTATGGGCAGCTGGCTGGACTTCGGTTCTATTGCCGGGTTTGTGCTGGGTGCGGGCgtggt  <  2:193914/113‑1 (MQ=255)                                                                                                                            aCCGGGGCGCCGATATTTGTTGCGGAATACTCCCCTGACCGTAAACGTGGCTTTATGGGCAGCTGGCTGGACTTCGGTTCTATTGCCGGGTTTGTGCTGGGTGCGGGCgtggt  >  1:193914/1‑113 (MQ=255)                                                                                                                                     |                                                                                                        ATTATGTCGATCAGTACGTTCTGTATTGGCTTAATACCGTCCTACGACACGATTGGTATTTGGGCACCGATTCTGCTGTTGATCTGTAAGATGGCACAAGGTTTCTCGGTCGGCGGTGAATATACCGGGGCGTCGATATTTGTTGCGGAATACTCCCCTGACCGTAAACGTGGCTTTATGGGCAGCTGGCTGGACTTCGGTTCTATTGCCGGGTTTGTGCTGGGTGCGGGCGTGGT  >  NZ_CP009273/4320631‑4320866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑