Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,389,340	A→G	E91G (GAA→GGA)	miaA →	tRNA (adenosine(37)‑N6)‑dimethylallyltransferase MiaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,389,340	0	A	G	100.0%	93.5 / NA	26	E91G (GAA→GGA)	miaA	tRNA (adenosine(37)‑N6)‑dimethylallyltransferase MiaA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/17);  total (9/17)

TTTACAAAGGGATGGATATCGGGACGGCGAAGCCGAACGCTGAAGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGAAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAGGCATTGCTGGAAGGGTTGTCGCCGCTACCGTCGGCAGACCCGGAAGTA  >  NZ_CP009273/4389205‑4389455                                                                                                                                        |                                                                                                                    tttACAAAGGGATGGATATCGGGACGGCGAAGCCGAACGCTGAAGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGgaa                                                                                                                   <  2:444568/138‑1 (MQ=255)          ggatggatATCGGGACGGCGAAGCCGAACGCTGAAGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGatat                                                                                                         >  1:325729/1‑139 (MQ=255)            atggatATCGGGACGGCGAAGCCGAACGCTGAAGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCATACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCa                                                                                                       >  1:234429/1‑139 (MQ=255)                      ggACGGCGAAGCCGAACGCTGAAGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCggcggg                                                                                             >  1:469764/1‑139 (MQ=255)                        aCGGCGAAGCCGAACGCTGAAGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCggcggggc                                                                                           <  2:70023/139‑1 (MQ=255)                            cGAAGCCGAACGCTGAAGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGAt                                                                                       <  1:323445/139‑1 (MQ=255)                             gAATCCGAACGCTGAAGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGATc                                                                                      <  1:385848/139‑1 (MQ=255)                                gCCGAACGCTGAAGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCa                                                                                   <  1:317721/139‑1 (MQ=255)                                 ccGAACGCTGAAGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCAc                                                                                  <  2:70894/139‑1 (MQ=255)                                           aaGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCCCTGTTAGTggg                                                                        >  2:65912/1‑139 (MQ=255)                                                       ccgcgccgcACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGTACGAtgtt                                                            <  1:264501/139‑1 (MQ=255)                                                        cgcgccgcACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCa                                                                                   >  1:165366/1‑115 (MQ=255)                                                        cgcgccgcACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCa                                                                                   <  2:165366/115‑1 (MQ=255)                                                        cgcgccgcACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGTACGAtgttg                                                           <  1:42766/139‑1 (MQ=255)                                                               cACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCa                                                    <  2:436310/139‑1 (MQ=255)                                                                    aTTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAGGCa                                               >  1:471793/1‑139 (MQ=255)                                                                     ttGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAGGCAt                                              <  1:327550/139‑1 (MQ=255)                                                                     ttGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAGGCAt                                              <  2:492981/139‑1 (MQ=255)                                                                       gCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAGGCAtt                                             <  2:295650/138‑1 (MQ=255)                                                                                         gTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAGGCATTGCTGGAAGGGTTGTcgccg                          <  2:325729/139‑1 (MQ=255)                                                                                         gTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAGGCATTGCTGGAAGGGTTGTcgccg                          <  2:471716/139‑1 (MQ=255)                                                                                          tCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAGGCATTGCTGGaa                                      <  2:203962/126‑1 (MQ=255)                                                                                          tCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAGGCATTGCTGGaa                                      >  1:203962/1‑126 (MQ=255)                                                                                                    aCTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAGGCATTGCTGGAAGGGTTGTCGCCGCTACCGTCgg                >  1:16610/1‑138 (MQ=255)                                                                                                          ctgctgATTTTCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGGGGGCGGTACGATGGTGTATTTCAAGGCATTGCTGGAAGGGGTGTCGCCGCTACCGTCGGCAGAccc         >  2:145167/1‑139 (MQ=255)                                                                                                                 ttttCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAGGCATTGCTGGAAGGGTTGTCGCCGCTACCGTCGGCAGACCCGGAAGTa  <  2:471793/139‑1 (MQ=255)                                                                                                                 ttttCGCCGCGATGCGCTGGCGGGAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAGGCATTGCTGGAAGGGTTGTCGCCGCTACCGTCGGCAGACCCGGAAGTa  <  2:469764/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                    TTTACAAAGGGATGGATATCGGGACGGCGAAGCCGAACGCTGAAGAGTTACTCGCCGCGCCGCACCGATTGCTGGATATTCGCGATCCGTCGCAGGCTTACTCGGCTGCTGATTTTCGCCGCGATGCGCTGGCGGAAATGGCCGATATCACCGCGGCGGGGCGGATCCCACTGTTAGTGGGCGGTACGATGTTGTATTTCAAGGCATTGCTGGAAGGGTTGTCGCCGCTACCGTCGGCAGACCCGGAAGTA  >  NZ_CP009273/4389205‑4389455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑