Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,401,268	T→G	G50G (GGT→GGG)	yjfK →	YjfK family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,401,268	0	T	G	100.0%	59.3 / NA	17	G50G (GGT→GGG)	yjfK	YjfK family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (9/8);  total (9/8)

GCGTCTGTTTGGCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGA  >  NZ_CP009273/4401136‑4401393                                                                                                                                     |                                                                                                                              gCGTCTGTTTGGCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGaa                                                                                                                         >  2:51635/1‑139 (MQ=255)  cGTCTGTTTGGCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAAt                                                                                                                        >  1:406882/1‑139 (MQ=255)            gCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAgc                                                                                                              <  2:437597/139‑1 (MQ=255)                     ataaGCCAGCTATCGCTCGTGGTCCGCTGGGTCTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTTCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAg                                                                                                     <  1:51635/139‑1 (MQ=255)                            aGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAg                                                                                                                            >  1:2915/1‑109 (MQ=255)                            aGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAg                                                                                                                            <  2:2915/109‑1 (MQ=255)                                        tGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGggcggc                                                                                  >  1:170357/1‑139 (MQ=255)                                                     cTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGAttt                                                                     >  2:440969/1‑139 (MQ=255)                                                        cATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGAttt                                                                     <  2:30439/136‑1 (MQ=255)                                                        cATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGAttt                                                                     >  1:30439/1‑136 (MQ=255)                                                                     gCTTTCCGCCCGACCCGTTACCGTTTCGTTTGCTGGAGGTTGATTTGCTGATTGCGCTGCCGGGGGGGGAATTTACGGTAGCCGCGGTCAGCCCCATCGATCTGGGCGGCGGTGGTCAGATTTTCCGCTCCTCCACTTc                                                     >  2:141627/1‑139 (MQ=255)                                                                                gACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGt                                          <  1:35158/139‑1 (MQ=255)                                                                                               cgtttGCTGGAAGAGGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAAt                            <  2:138777/138‑1 (MQ=255)                                                                                                        gAAGATGAATTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACcggc                   <  2:406882/138‑1 (MQ=255)                                                                                                               aaTTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAg            <  1:58904/138‑1 (MQ=255)                                                                                                                aTTGCTGATTGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGa           >  2:488468/1‑138 (MQ=255)                                                                                                                         tGCGCTGCCGGGGGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATtgatga  >  2:189415/1‑138 (MQ=255)                                                                                                                                     |                                                                                                                              GCGTCTGTTTGGCAAGGATAATAAGCCAGCTATCGCTCGTGGTCCGCTGGGACTTCATCTCAATAGTGGCTTTACGCTCGACACGTTAGCGTTTCGTTTGCTGGAAGATGAATTGCTGATTGCGCTGCCGGGTGAGGAATTTACGGTAGCCGCCGTCAGCCACATCGATCTGGGCGGCGGTAGTCAGATTTTCCGCTACTACACTTCAGGCGATGAGTTTCTGCAAATCAATACCACCGGCGGCGAAGATATTGATGA  >  NZ_CP009273/4401136‑4401393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑