Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,589,054	T→G	T69P (ACC→CCC)	opgB ←	phosphatidylglycerol‑‑membrane‑oligosaccharide glycerophosphotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,589,054	0	T	G	95.5%	71.2 / ‑5.6	22	T69P (ACC→CCC)	opgB	phosphatidylglycerol‑‑membrane‑oligosaccharide glycerophosphotransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base G (7/14);  minor base A (1/0);  total (8/14)
Fisher's exact test for biased strand distribution p-value = 3.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCAGGCTGTAACCAAAATGGTGCGGATGATGGCGACGACGGCGCAGGATCCAGCCCAGCGCACCGAACACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTT  >  NZ_CP009273/4588921‑4589163                                                                                                                                      |                                                                                                              agcagGCTGTAACCAAAATGGTGCGGATGATGGCGACGACGGCGCAGGATCCAGCCCAGCGCACCGAACACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGACAGGc                                                                                                          >  2:104407/1‑139 (MQ=255)          tAACCAAAATGGTGCGGATGATGGCGACGACGGCGCAGGATCCAGCCCAGCGCACCGAACACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGGCAGGCTGTTGGTTa                                                                                                 <  2:54835/139‑1 (MQ=255)          tAACCAAAATGGTGCGGATGATGGCGACGACGGCGCAGGATCCAGCCCAGCGCACCGAACACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGGCAGGCTGTTGGTTa                                                                                                 <  2:395048/139‑1 (MQ=255)          tAACCAAAATGGTGCGGATGATGGCGACGACGGCGCAGGATCCAGCCCAGCGCACCGAACACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGGCAGGCTGTTGGTTa                                                                                                 <  2:229878/139‑1 (MQ=255)          tAACCAAAATGGTGCGGATGATGGCGACGACGGCGCAGGATCCAGCCCAGCGCACCGAACACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGGCAGGCTGTTGGTTa                                                                                                 <  2:134722/139‑1 (MQ=255)            aCCAAAATGGTGCGGATGATGGCGACGACGGCGCAGGATCCAGCCCAGCGCACCGAACACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGGCAGGCTGTTGGTTAAg                                                                                               <  2:391517/139‑1 (MQ=255)                          gatgatGGCGACGACGGCGCAGGATCCAGCCCAGCGCACCGAACACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGGCAGGCTGTTGGTTAAGGTATAGAGAACcgc                                                                                 <  2:368642/139‑1 (MQ=255)                                ggCGACGACGGCGCAGGATCCAGCCCAGCGCACCGAACACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGGCAGGCTGTTgg                                                                                                    <  1:385829/114‑1 (MQ=255)                                ggCGACGACGGCGCAGGATCCAGCCCAGCGCACCGAACACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGGCAGGCTGTTgg                                                                                                    >  2:385829/1‑114 (MQ=255)                                           cgcAGGATCCAGCCCAGCGCACCGAACACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGGCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGc                                                                <  2:157344/139‑1 (MQ=255)                                                          agcGCACCGAACACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGGCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGt                                                    >  2:145619/1‑136 (MQ=255)                                                          agcGCACCGAACACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGGCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGt                                                    <  1:145619/136‑1 (MQ=255)                                                            cgcACCGAACACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGGCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATc                                                                  <  2:453036/120‑1 (MQ=255)                                                            cgcACCGAACACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGGCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTg                                               >  2:481671/1‑139 (MQ=255)                                                            cgcACCGAACACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGGCAGGCTGTTGGTTAAGGCATAGAGAACCGCGTCGTTAATACCATc                                                                  >  1:453036/1‑120 (MQ=255)                                                                                            tGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGGCAGGCTGTTGGTTAAg                                                                                               <  1:478916/59‑1 (MQ=255)                                                                                            tGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGGCAGGCTGTTGGTTAAg                                                                                               >  2:478916/1‑59 (MQ=255)                                                                                            tGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGGCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAg               >  1:225680/1‑139 (MQ=255)                                                                                                    cccGGCAAAATGTATTTGCTGACGCCAGCACCGGGCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCa       <  1:481671/139‑1 (MQ=255)                                                                                                         cAAAATGTATTTGCTGACGCCAGCACCGGGCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGtt  <  2:225680/139‑1 (MQ=255)                                                                                                                              aGCACCGGGCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCa       <  1:115849/113‑1 (MQ=255)                                                                                                                              aGCACCGGGCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCa       >  2:115849/1‑113 (MQ=255)                                                                                                                                      |                                                                                                              AGCAGGCTGTAACCAAAATGGTGCGGATGATGGCGACGACGGCGCAGGATCCAGCCCAGCGCACCGAACACCGCTGTCAGCCCCAGCACAATGCCGATACCCGGCAAAATGTATTTGCTGACGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTT  >  NZ_CP009273/4588921‑4589163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑