Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	338,646	A→T	M18L (ATG→TTG)	yahK →	NADPH‑dependent aldehyde reductase YahK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	338,646	0	A	T	100.0%	113.2 / NA	30	M18L (ATG→TTG)	yahK	NADPH‑dependent aldehyde reductase YahK
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (14/16);  total (14/16)

GGCTATATTCAATGGACGCGTTTTGCCGCGATGACATATCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGATGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGATCTCCACCAGGTCCGTTCCGAGTGGGCGGGGA  >  NZ_CP009273/338514‑338754                                                                                                                                     |                                                                                                             ggCTATATTCAATGGACGCGTTTTGCCGCGATGACATATCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGat                                                                                                         <  1:19057/138‑1 (MQ=255)                aCGCGTTTTGCCGCGATGACATATCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAAc                                                                                         >  2:88187/1‑139 (MQ=255)                aCGCGTTTTGCCGCGATGACATATCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAAc                                                                                         <  1:217038/139‑1 (MQ=255)                aCGCGTTTTGCCGCGATGACATATCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAAc                                                                                         <  2:433244/139‑1 (MQ=255)                     ttttGCCGCGATGACATATCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCCCCCGGCGTGAACCGGGa                                                                                    <  2:243517/139‑1 (MQ=255)                                          ggCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAaaatcgaa                                                               <  1:372683/139‑1 (MQ=255)                                              ttGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAaaatcgaaatcg                                                           >  2:166081/1‑139 (MQ=255)                                               tGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGc                                                          >  1:253115/1‑139 (MQ=255)                                                gCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCt                                                         >  2:83529/1‑139 (MQ=255)                                                           aTAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGt                                              >  1:313533/1‑139 (MQ=255)                                                           aTAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGt                                              >  1:496033/1‑139 (MQ=255)                                                                   tCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCAtt                                      >  2:323475/1‑139 (MQ=255)                                                                     aGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTc                                     <  2:496033/138‑1 (MQ=255)                                                                         gTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGAtc                                <  2:313533/139‑1 (MQ=255)                                                                              cacaATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGATCTccac                           <  1:410896/139‑1 (MQ=255)                                                                                 aaTGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGata                                                                                                        >  2:172026/1‑59 (MQ=255)                                                                                 aaTGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGata                                                                                                        <  1:172026/59‑1 (MQ=255)                                                                                 aaTGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGATCTCCACCAg                        >  1:222290/1‑139 (MQ=255)                                                                                  aTGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGATCTCCACCAgg                       >  1:286299/1‑139 (MQ=255)                                                                                   tGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGATCTCCACCAGGt                      <  1:334644/139‑1 (MQ=255)                                                                                        aTCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGAc                                                                                   <  1:441375/73‑1 (MQ=255)                                                                                        aTCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGAc                                                                                   >  2:441375/1‑73 (MQ=255)                                                                                           aaaGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGATCTCCACCAGGTCCGTTcc               >  2:302172/1‑138 (MQ=255)                                                                                               cTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGc                                                <  2:106341/101‑1 (MQ=255)                                                                                               cTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGc                                                >  1:106341/1‑101 (MQ=255)                                                                                                        cATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGATCTCCACCAGGTCCGTTCCGAGTGGGCGGGGa  <  1:63853/138‑1 (MQ=255)                                                                                                        cATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGATCTCCACCAGGTCCGTTCCGAGTGGGCGGGGa  <  2:222290/138‑1 (MQ=255)                                                                                                        cATATTCCGCTAAACAACCACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGATCTCCACCAGGTCCGTTCCGAGTGGGCGGGGa  <  1:357348/138‑1 (MQ=255)                                                                                                                         ccACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCaaa                                                                     >  2:29992/1‑54 (MQ=255)                                                                                                                         ccACTTGAACCGTTGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCaaa                                                                     <  1:29992/54‑1 (MQ=255)                                                                                                                                     |                                                                                                             GGCTATATTCAATGGACGCGTTTTGCCGCGATGACATATCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGATGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGATCTCCACCAGGTCCGTTCCGAGTGGGCGGGGA  >  NZ_CP009273/338514‑338754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑