Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	351,491	T→G	S287A (TCG→GCG)	codB →	cytosine permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	351,491	0	T	G	100.0%	92.0 / NA	26	S287A (TCG→GCG)	codB	cytosine permease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (16/10);  total (16/10)

GGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAACGATAACGCACTCTATGCGTCGGGTTTAGGTTTCGCCAACATTACCGGGATGTCGAGCAAAACCCTTTCGGTAATCAACGGTATTATCGGTACGGTCTGCGCATTATGGCTGTATAACAATTTTGTCGGCTGGTTGACC  >  NZ_CP009273/351383‑351610                                                                                                             |                                                                                                                        ggCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAACGATAACGCACTCTATGCGGCGGGTTTAGGTTTCGCCAACATTACCgg                                                                                             >  2:85097/1‑137 (MQ=255) ggCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAACGATAACGCACTCTATGCGGCGGGTTTAGGTTTCGCCAACATTACCGGGa                                                                                           >  1:244168/1‑139 (MQ=255)      ggCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAACGATAACGCACTCTATGCGGCGGGTTTAGGTTTCGCCAACATTACCGGGATGTCg                                                                                      >  1:414920/1‑139 (MQ=255)      ggCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAACGATAACGCACTCTATGCGGCGGGGTTAGGGTTCGCCAACATTACCGGGATGTCg                                                                                      >  2:326275/1‑139 (MQ=255)                         atgatTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAACGATAACGCACTCTATGCGGCGGGTTTAGGTTTCGCCAACATTACCGGGATGt                                                                                        <  1:218478/118‑1 (MQ=255)                         atgatTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAACGATAACGCACTCTATGCGGCGGGTTTAGGTTTCGCCAACATTACCGGGATGt                                                                                        >  2:218478/1‑118 (MQ=255)                         atgatTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAACGATAACGCACTCTATGCGGCGGGTTTAGGTTTCGCCAACATTACCGGGATGTCGAGCAAAACCCTTTCGGTaa                                                                   >  2:452420/1‑139 (MQ=255)                         atgatTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAACGATAACGCACTCTATGCGGCGGGTTTAGGTTTCGCCAACATTACCGGGATGTCGAGCAAAACCCTTTCGGTaa                                                                   >  2:230257/1‑139 (MQ=255)                          tgatTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAACGATAACGCACTCTATGCGGCGGGGTTAGGGTTCGCCAACAATACCGGGATGTCGAGCAAAACCCTTTCGGGAAt                                                                  >  2:71433/1‑139 (MQ=255)                            atTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAACGATAACGCACTCTATGCGGCGGGTTTAGGTTTCGCCAACATTACCGGGATGTCGAGCAAAAACCTTTCGGTAATc                                                                 >  2:497175/1‑138 (MQ=255)                                cTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAACGATAACGCACTCTATGCGGCGGGTTTAGGTTTCGCCAACATTACCGGGATGTCGAGCAAAACCCTTTCGGTAATCAACg                                                             <  1:85097/138‑1 (MQ=255)                                          gctgctgcCTGCGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAACGATAACGCACTCTATGCGGCGGGTTTAGGGTTCGCCAACATTACCGGGGTGTCGAGCAAAACCCTTTCGGGAATCAACGGGATTATTgg                                                   >  2:256817/1‑138 (MQ=255)                                          gctgctgcCTGCGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAACGATAACGCACTCTATGCGGCGGGGTTAGGTTTCGCCAACATTAACGGGATGTCGAGCAAAAACCTTTCGGTAATCAACGGGATTATCgg                                                   >  2:142154/1‑138 (MQ=255)                                           ctgctgcCTGCGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAACGATAACGCACTCTATGCGGCGGGTTTAGGTTTCGCCAACATTACCGGGATGTCGAGCAAAACCCTTTCGGTAATCAACGGTATTATCGGt                                                  <  1:142154/138‑1 (MQ=255)                                           ctgctgcCTGCGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAACGATAACGCACTCTATGCGGCGGGTTTAGGTTTCGCCAACATTACCGGGATGTCGAGCAAAACCCTTTCGGTAATCAACGGTATTATCGGt                                                  <  2:270083/138‑1 (MQ=255)                                                 cctgcGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAACGATAACGCACTCTATGCGGCGGGTTTAGGTTTCGCCAACATTACCGGGATGTCGAGCAAAACCCTTTCGGTAATCAACGGTATTATCGGTACGGt                                             <  1:246539/137‑1 (MQ=255)                                                  ctgcGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAACGATAACGCACTCTATGCGGCGGGTTTAGGTTTCGCCAACATTACCGGGATGTCGAGCAAAACCCTTTCGGTAATCAACGGTATTATCGGTACGGTCTg                                          <  2:72325/139‑1 (MQ=255)                                                           tggtgCTGGGGCTGAATATCTGGACCACCAACGATAACGCACTCTATGCGGCGGGTTTAGGTTTCGCCAACATTACCGGGATGTCGAGCAAAACCCTTTCGGTAATCAACGGTATTATCGGTACGGTCTGCGCATTAt                                  <  1:99477/138‑1 (MQ=255)                                                           tggtgCTGGGGCTGAATATCTGGACCACCAACGATAACGCACTCTATGCGGCGGGTTTAGGTTTCGCCAACATTACCGGGATGTCGAGCAAAACCCTTTCGGTAATCAACGGGATTATCGGAACGGTCTGCGCATTATg                                 >  2:371797/1‑139 (MQ=255)                                                             gtgCTGGGGCTGAATATCTGGACCACCAACGATAACGCACTCTATGCGGCGGGTTTAGGTTTCGCCAACATTACCGGGATGTCGAGCAAAACCCTTTCGGTAATCAACGGTATTATCGGTACGGTCTGCGCATTATgg                                <  1:452420/138‑1 (MQ=255)                                                                  ggggCTGAATATCTGGACCACCAACGATAACGCACTCTATGCGGCGGGTTTAGGTTTCGCCAACATTACCGGGATGTCGAGCAAAACCCTTTCGGTAATCAACGGTATTATCGGTACGGTCTGCGCATTATGGCTGta                           >  1:449791/1‑138 (MQ=255)                                                                  ggggCTGAATATCTGGACCACCAACGATAACGCACTCTATGCGGCGGGTTTAGGTTTCGCCAACATTACCGGGAAGTCGAGCAAAAACCTTTCGGTAATCAAAGGGATTATCGGTACGGTCTGCGCATTATTGCTGta                           >  1:228199/1‑138 (MQ=255)                                                                       tGAATATCTGGACCACCAACGATAACGCACTCTATGCGGCGGGTTTAGGTTTCGCCAACATTACCGGGATGTCGAGCaa                                                                                 <  2:123599/79‑1 (MQ=255)                                                                       tGAATATCTGGACCACCAACGATAACGCACTCTATGCGGCGGGTTTAGGTTTCGCCAACATTACCGGGATGTCGAGCaa                                                                                 >  1:123599/1‑79 (MQ=255)                                                                               tGGACCACCAACGATAACGCACTCTATGCGGCGGGTTTAGGTTTCGCCAACATTACCGGGATGTCGAGCAAAACCCTTTCGGTAATCAACGGTATTATCGGTACGGTCTGCGCATTATGGCTGTATAACAATTTTGTCg             >  1:430465/1‑139 (MQ=255)                                                                                          cGATAACGCACTCTATGCGGCGGGTTTAGGTTTCGCCAACATTACCGGGATGTCGAGCAAAACCCTTTCGGTAATCAACGGTATTATCGGTACGGTCTGCGCATTATGGCTGTATAACAATTTTGTCGGCTGGTTGAcc  <  1:497175/139‑1 (MQ=255)                                                                                                             |                                                                                                                        GGCATGGCGGATATCTCTGATGTGATGATTGCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAACGATAACGCACTCTATGCGTCGGGTTTAGGTTTCGCCAACATTACCGGGATGTCGAGCAAAACCCTTTCGGTAATCAACGGTATTATCGGTACGGTCTGCGCATTATGGCTGTATAACAATTTTGTCGGCTGGTTGACC  >  NZ_CP009273/351383‑351610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑