Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	393,907	A→G	K194E (AAA→GAA)	yaiW →	surface‑exposed outer membrane lipoprotein YaiW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	393,907	0	A	G	100.0%	82.1 / NA	23	K194E (AAA→GAA)	yaiW	surface‑exposed outer membrane lipoprotein YaiW
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/11);  total (12/11)

CGCGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGAAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGC  >  NZ_CP009273/393771‑394034                                                                                                                                         |                                                                                                                                cgcgATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGgaa                                                                                                                               <  2:48256/139‑1 (MQ=255)   cgATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGGAAAt                                                                                                                             <  2:493932/139‑1 (MQ=255)       ttttGGCGATCTGATCAACATGGTGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTAACCGTGGGAAATGGa                                                                                                                          <  2:176131/138‑1 (MQ=255)                            ggTGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTc                                                                                                    >  2:82119/1‑139 (MQ=255)                                    tGGGGCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTcg                                                                                            >  1:31370/1‑139 (MQ=255)                                       ggCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTcgccg                                                                                         >  2:260659/1‑139 (MQ=255)                                           gACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCgcggc                                                                                     <  1:271406/139‑1 (MQ=255)                                                  tttGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTtg                                                                               <  1:327406/138‑1 (MQ=255)                                                  tttGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTtg                                                                               <  1:82119/138‑1 (MQ=255)                                                            tCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGttt                                                                         <  1:355367/134‑1 (MQ=255)                                                            tCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGttt                                                                         >  2:355367/1‑134 (MQ=255)                                                              aaTCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACtt                                                                  <  1:134710/139‑1 (MQ=255)                                                                                      aTGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGc                                            >  1:40556/1‑137 (MQ=255)                                                                                      aTGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGc                                            >  1:383115/1‑137 (MQ=255)                                                                                      aTGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGGTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGc                                            >  1:253236/1‑137 (MQ=255)                                                                                            gTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAg                                         <  1:466775/134‑1 (MQ=255)                                                                                            gTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAg                                         >  2:466775/1‑134 (MQ=255)                                                                                               aGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAgcg                                 <  2:383115/139‑1 (MQ=255)                                                                                                                 cAGCATACCAAAGGGTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCa                               <  2:327176/123‑1 (MQ=255)                                                                                                                 cAGCATACCAAAGGGTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCa                               >  1:327176/1‑123 (MQ=255)                                                                                                                              ggTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACgc  >  2:43770/1‑139 (MQ=255)                                                                                                                              ggTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACgc  >  2:453161/1‑139 (MQ=255)                                                                                                                              ggTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACg   <  2:126749/138‑1 (MQ=255)                                                                                                                              ggTATCCGTGGGAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACg   >  1:126749/1‑138 (MQ=255)                                                                                                                                         |                                                                                                                                CGCGATTTTTGACGATCTGATCAACATGGTGCCAATGGGGCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGCATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGAAAATGGACGGTACAGTCCGTCAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGC  >  NZ_CP009273/393771‑394034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑