Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	415,362	T→C	S106P (TCC→CCC)	brnQ →	branched‑chain amino acid transporter carrier protein BrnQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	415,362	0	T	C	100.0%	28.2 / NA	9	S106P (TCC→CCC)	brnQ	branched‑chain amino acid transporter carrier protein BrnQ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (4/5);  total (4/5)

CCAATCGGTAAAGTCGCTGGCGTACTGCTGGCAACGGTTTGTTACCTGGCGGTGGGGCCGCTTTTCGCTACGCCGCGTACAGCTACCGTTTCCTTTGAAGTGGGGATTGCGCCGCTGACGGGTGATTCCGCGCTGCCGCTGTTTATCTACAGCCTGGTCTATTTCGC  >  NZ_CP009273/415272‑415438                                                                                           |                                                                             ccAATCGGTAAAGTCGCTGGCGTACTGCTGGCAACGGTTTGTTACCTGGCGGTGGGGCCGCTTTTCGCTACGCCGCGTACAGCTACCGTTCCCTTTGAAGTGGGGATTGCGCCGCTGACGGGTGATTCCGCGCTGCCGc                              <  1:161440/139‑1 (MQ=255) ccAATCGGTAAAGTCGCTGGCGTACTGCTGGCAACGGTTTGTTACCTGGCGGTGGGGCCGCTTTTCGCTACGCCGCGTACAGCTACCGTTCCCTTTGAAGTGGGGATTGCGCCGCTGACGGGTGATTCCGCGCTGCCGc                              >  2:413633/1‑139 (MQ=255)           aaGTCGCTGGCGTACTGCTGGCAACGGTTTGTTACCTGGCGGTGGGGCCGCTTTTCGCTACGCCGCGTACAGCTACCGTTCCCTTTGAAGTGGGGATTGCGCCGCTGACGGGTGATTCCGCGCTGCCGCTGTTTATCTa                    <  1:413633/139‑1 (MQ=255)                                      tttGTTACCTGGCGGTGGGGCCGCTTTTCGCTACGCCGCGTACAGCTACCGTTCCCTTTGAAGTGGGGATTGCGCCGCTGACGGGTGATTCCGCGCTGCCGCTGTTTATCTACAGCCTGGTCTATTTCGc  >  1:1092/1‑130 (MQ=255)                                      tttGTTACCTGGCGGTGGGGCCGCTTTTCGCTACGCCGCGTACAGCTACCGTTCCCTTTGAAGTGGGGATTGCGCCGCTGACGGGTGATTCCGCGCTGCCGCTGTTTATCTACAGCCTGGTCTATTTCGc  <  2:1092/130‑1 (MQ=255)                                                       gggCCGCTTTTCGCTACGCCGCGTACAGCTACCGTTCCCTTTGAAGTGGGGATTGCGCCGCTGACGGGTGATTCCGCGCTGCCGc                              >  1:178466/1‑85 (MQ=255)                                                       gggCCGCTTTTCGCTACGCCGCGTACAGCTACCGTTCCCTTTGAAGTGGGGATTGCGCCGCTGACGGGTGATTCCGCGCTGCCGc                              <  2:178466/85‑1 (MQ=255)                                                                  cGCTACGCCGCGTACAGCTACCGTTCCCTTTGAAGTGGGGATTGCGCCGCTGACGGGTGATTCCGCGCTGCCGc                              >  1:473651/1‑74 (MQ=255)                                                                  cGCTACGCCGCGTACAGCTACCGTTCCCTTTGAAGTGGGGATTGCGCCGCTGACGGGTGATTCCGCGCTGCCGc                              <  2:473651/74‑1 (MQ=255)                                                                                           |                                                                             CCAATCGGTAAAGTCGCTGGCGTACTGCTGGCAACGGTTTGTTACCTGGCGGTGGGGCCGCTTTTCGCTACGCCGCGTACAGCTACCGTTTCCTTTGAAGTGGGGATTGCGCCGCTGACGGGTGATTCCGCGCTGCCGCTGTTTATCTACAGCCTGGTCTATTTCGC  >  NZ_CP009273/415272‑415438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑