Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	478,146	A→C	F572V (TTT→GTT)	acrB ←	efflux RND transporter permease AcrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	478,146	0	A	C	100.0%	86.9 / NA	24	F572V (TTT→GTT)	acrB	efflux RND transporter permease AcrB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (13/11);  total (13/11)

AAGCCGTTAACGGCGAACACCGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATGCGTTACCTCATTGAGCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAAACACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAACGCCCCGTACTGCGCAGAATACCGCCTACGCT  >  NZ_CP009273/478016‑478272                                                                                                                                   |                                                                                                                               aagccgTTAACGGCGAACACCGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATGCGTTACCTCATTGAGCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCt                                                                                                                        >  2:16723/1‑139 (MQ=255)              cGAACACCGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATGCGTTACCTCATTGAGCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTgg                                                                                                           >  2:210896/1‑139 (MQ=255)                       aCTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATGCGTTACCTCATTGAGCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCaagaag                                                                                                    >  1:482536/1‑137 (MQ=255)                             cGTTGTTCTTTTCTTTGGTCAGATAGTAATGCGTTACCTCATTGAGCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTgg                                                                                            >  2:373158/1‑139 (MQ=255)                                        tctttGGTCAGATAGTAATGCGTTACCTCATTGAGCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACg                                                                                  <  1:273020/138‑1 (MQ=255)                                              gTCAGATAGTAATGCGTTACCTCATTGAGCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGAt                                                                           <  1:63930/139‑1 (MQ=255)                                                               tACCTCATTGAGCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCAc                                                           >  1:316223/1‑138 (MQ=255)                                                                     aTTGAGCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATc                                                    <  1:373158/139‑1 (MQ=255)                                                                          gCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATa                                               <  2:482536/139‑1 (MQ=255)                                                                           cACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGACCATGCCGACCACGATGATCAGATa                                               >  2:206117/1‑138 (MQ=255)                                                                                   gtgtACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCa                                          <  1:447619/135‑1 (MQ=255)                                                                                   gtgtACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCa                                          >  2:447619/1‑135 (MQ=255)                                                                                     gtACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAg                                                                                                 >  1:267662/1‑78 (MQ=255)                                                                                     gtACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAg                                                                                                 <  2:267662/78‑1 (MQ=255)                                                                                      tACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGt                                    >  2:138384/1‑138 (MQ=255)                                                                                      tACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTa                                   <  2:35207/139‑1 (MQ=255)                                                                                      tACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTa                                   <  1:16723/139‑1 (MQ=255)                                                                                        cGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTa                                   >  2:236316/1‑137 (MQ=255)                                                                                        cGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTa                                   <  1:236316/137‑1 (MQ=255)                                                                                            ccTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAACGccc                             >  2:293843/1‑139 (MQ=255)                                                                                                             ggCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATGCAGCACCAGGTAACGCCCCGTa                         <  1:237063/126‑1 (MQ=255)                                                                                                             ggCAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAACGCCCCGTa                         >  2:237063/1‑126 (MQ=255)                                                                                                               cAGCTGAACCATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAACGCCCCGTACTGCGCAGAATACCg          >  2:171342/1‑139 (MQ=255)                                                                                                                       ccATGGTCATAACCACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAACGCCCCGTACTGCGCAGAATACCGCCTACGCt  <  1:450255/139‑1 (MQ=255)                                                                                                                                   |                                                                                                                               AAGCCGTTAACGGCGAACACCGACTCAACGTTGTTCTTTTCTTTGGTCAGATAGTAATGCGTTACCTCATTGAGCACTTTCTGTGTACGTTCCTGCGTTGCACCTGCTGGCAGCTGAACCATGGTCATAAACACGCCCTGGTCCTCATCTGGCAAGAAGGAGCTTGGCAGACGCACGAACAGATAGGCCATGCCGACCACGATGATCAGATACAGCACCAGGTAACGCCCCGTACTGCGCAGAATACCGCCTACGCT  >  NZ_CP009273/478016‑478272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑