Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	859,589	A→G	A164A (GCA→GCG)	fsa →	fructose‑6‑phosphate aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	859,589	0	A	G	100.0%	48.8 / NA	15	A164A (GCA→GCG)	fsa	fructose‑6‑phosphate aldolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/10);  total (5/10)

GAATATGTTGCGCCTTACGTTAATCGTATTGATGCTCAGGGCGGTAGCGGCATTCAGACTGTGACCGACTTACACCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGGCAGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTACTGGCAGGATGTGAATCAATTACTCTGCCACTGGATGTGGCA  >  NZ_CP009273/859473‑859670                                                                                                                     |                                                                                  gAATATGTTGCGCCTTACGTTAATCGTATTGATGCTCAGGGCGGTAGCGGCATTCAGACTGTGACCGACTTACACCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGGCGGCGAGTTTCAAAACCCCGCGTc                                                             <  2:475469/139‑1 (MQ=255)           cgcCTTACGTTAATCGTATTGATGCTCAGGGCGGTAGCGGCATTCAGACTGTGACCGACTTACACCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGGCGGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGa                                                   <  1:368272/139‑1 (MQ=255)            gcCTTACGTTAATCGTATTGATGCTCAGGGCGGTAGCGGCATTCAGACTGTGACCGACTTACACCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGGCGGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGAc                                                  <  1:163869/139‑1 (MQ=255)                    ttAATCGTATTGATGCTCAGGGCGGTAGCGGCATTCAGACTGTGACCGACTTACACCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGGCGGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTACt                                          <  1:27095/139‑1 (MQ=255)                            aTTGATGCTCAGGGCGGTAGCGGCATTCAGACTGTGACCGACTTACACCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGGCGGCGAGTTTCAAAACCCCGCGTc                                                             <  1:338751/112‑1 (MQ=255)                            aTTGATGCTCAGGGCGGTAGCGGCATTCAGACTGTGACCGACTTACACCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGGCGGCGAGTTTCAAAACCCCGCGTc                                                             >  2:338751/1‑112 (MQ=255)                                        ggCGGTAGCGGCATTCAGACTGTGACCGACTTACACCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGGCGGCGAGTTTCAAAACCCCGCGTc                                                             >  1:470774/1‑100 (MQ=255)                                        ggCGGTAGCGGCATTCAGACTGTGACCGACTTACACCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGGCGGCGAGTTTCAAAACCCCGCGTc                                                             <  2:470774/100‑1 (MQ=255)                                             tAGCGGCATTCAGACTGTGACCGACTTACACCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGGCGGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTACTGGCAGGATGTGAATCAATTACTCTg                 >  1:149447/1‑139 (MQ=255)                                                cGGCATTCAGACTGTGACCGACTTACACCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGGCGGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTACTGGCAGGATGTGAATCAATTACTCTGCCa              <  2:149447/139‑1 (MQ=255)                                                              tgACCGACTTACACCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGGCGGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTACTGGCAGGATGTGAATCAATTACTCTGCCACTGGATGTGGca  <  2:302970/137‑1 (MQ=255)                                                                              gTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGGCGGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTACTGGCAGGATGTGAATCAATTACTCTGCCa              >  1:50495/1‑109 (MQ=255)                                                                              gTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGGCGGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTACTGGCAGGATGTGAATCAATTACTCTGCCa              <  2:50495/109‑1 (MQ=255)                                                                               ttATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGGCGGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTACTGGCAGGATGTGa                              <  1:492963/92‑1 (MQ=255)                                                                               ttATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGGCGGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTACTGGCAGGATGTGa                              >  2:492963/1‑92 (MQ=255)                                                                                                                     |                                                                                  GAATATGTTGCGCCTTACGTTAATCGTATTGATGCTCAGGGCGGTAGCGGCATTCAGACTGTGACCGACTTACACCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGGCAGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTACTGGCAGGATGTGAATCAATTACTCTGCCACTGGATGTGGCA  >  NZ_CP009273/859473‑859670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑