Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,103,672	C→T	T242T (ACG→ACA)	mdoC ←	glucans biosynthesis protein MdoC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,103,672	0	C	T	100.0%	54.6 / NA	15	T242T (ACG→ACA)	mdoC	glucans biosynthesis protein MdoC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/7);  total (8/7)

TCCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCATAGCGCTGGTTGAGTAAATAAGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAATGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATGACAATAAAATTGAACATGCCATTACTCAGAATGGGCGGATAC  >  NZ_CP009273/1103542‑1103798                                                                                                                                   |                                                                                                                               tCCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCATAGCGCTGGTTGAGTAAATAAGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGTGTGGTaaa                                                                                                                        <  2:197567/139‑1 (MQ=255)   cacaGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCATAGCGCTGGTTGAGTAAATAAGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGTGTGGTAAACa                                                                                                                      <  2:444846/139‑1 (MQ=255)            aGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCATAGCGCTGGTTGAGTAAATAAGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGTGTGGTAAACAAGGCttt                                                                                                               >  1:343589/1‑137 (MQ=255)                   tGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCATAGCGCTGGTTGAGTAAATAAGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGTGTGGTAAACAAGGCTTTAAGATGAgg                                                                                                      >  1:89398/1‑139 (MQ=255)                         tCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCATAGCGCTGGTTGAGTAAATAAGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGTGTGGTAAACAAGGCTTTAAGATGAGGGAAAAt                                                                                                >  2:279109/1‑139 (MQ=255)                                                 aGGCATCGCCACTGCCATAGCGCTGGTTGAGTAAATAAGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGTGTGGTAAACAAGGCTTTAAGATGAGGGAAAATGAAAGCCAGTGCGcc                                                                                 >  1:114328/1‑130 (MQ=255)                                                 aGGCATCGCCACTGCCATAGCGCTGGTTGAGTAAATAAGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGTGTGGTAAACAAGGCTTTAAGATGAGGGAAAATGAAAGCCAGTGCGcc                                                                                 <  2:114328/130‑1 (MQ=255)                                                        gCCACTGCCATAGCGCTGGTTGAGTAAATAAGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGTGTGGTAAACAAGGCTTTAAGATGAGGGAAAATGAAAGCCAGTGCGCCGAGGATAAAGAACGGc                                                                 <  2:89398/139‑1 (MQ=255)                                                             tGCCATAGCGCTGGTTGAGTAAATAAGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGTGTGGTAAACAAGGCTTTAAGATGAGGGAAAATGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAAt                                                             >  2:296825/1‑138 (MQ=255)                                                                         ggTTGAGTAAATAAGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGTGTGGTAAACAAGGCTTTAAGATGAGGGAAAATGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTg                                                >  2:141680/1‑139 (MQ=255)                                                                                                  cAATGCTGCTGCAAGGGTACAGCCACGAGACGGTGTGGTAAACAAGGCTTTAAGATGAGGGAAAATGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATGACAATAAAATTGAACATGCCa                       <  1:279109/139‑1 (MQ=255)                                                                                                  cAATGCTGCTGCAAGGGTACAGCCACGAGACGGTGTGGTAAACAAGGCTTAAAGATGAGGGAAAATGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATGACAATAAAATTGAACATGCCa                       <  1:141680/139‑1 (MQ=255)                                                                                                               aGGGTACAGCCACGAGACGGTGTGGTAAACAAGGCTTTAAGATGAGGGAAAATGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATGACAATAAAATTGAACATGCCATTACTCAGAATgg          >  2:497623/1‑139 (MQ=255)                                                                                                                            gagaCGGTGTGGTAAACAAGGCTTTAAGATGAGGGAAAATGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATGACAATAAAATTGAACATGCCATTACTCAGAATGGGCGGATac  >  1:373979/1‑134 (MQ=255)                                                                                                                            gagaCGGTGTGGTAAACAAGGCTTTAAGATGAGGGAAAATGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATGACAATAAAATTGAACATGCCATTACTCAGAATGGGCGGATac  <  2:373979/134‑1 (MQ=255)                                                                                                                                   |                                                                                                                               TCCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCATAGCGCTGGTTGAGTAAATAAGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTAAACAAGGCTTTAAGATGAGGGAAAATGAAAGCCAGTGCGCCGAGGATAAAGAACGGCAAATAAAACAGCGTTTGCATGACAATAAAATTGAACATGCCATTACTCAGAATGGGCGGATAC  >  NZ_CP009273/1103542‑1103798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑