| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,268,640 | A→G | L226L (TTA→TTG) | chaC → | glutathione‑specific gamma‑glutamylcyclotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,268,640 | 0 | A | G | 85.7% | 13.1 / ‑2.8 | 7 | L226L (TTA→TTG) | chaC | glutathione‑specific gamma‑glutamylcyclotransferase |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (2/4); total (3/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCACCCCATCAA > NZ_CP009273/1268519‑1268778 | cacaATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTGCGTCCGGGATTCGCCTg < 1:13656/139‑1 (MQ=255) cAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCACGCATAGTgggg > 2:403213/1‑139 (MQ=255) aTCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTGCGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCAGACg > 1:496881/1‑139 (MQ=255) aaaCTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTGCGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCAGACGcc < 2:496881/138‑1 (MQ=255) aCGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTGCGTc < 1:166384/75‑1 (MQ=255) aCGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTGCGTc > 2:166384/1‑75 (MQ=255) gATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTGCGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAAtgt < 1:208624/139‑1 (MQ=255) aCGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCAGACGCCACCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTTCATTGCGCCAGTTCCACAAGAGTACCGATTTCCACCCCatcaa > 2:406482/1‑139 (MQ=255) | CACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGATTTGCTGGTATCGGTAAAAAAACTGCTGGCGGAGAATTTTCCGGATGGTGTGTTACGTCCGGGATTCGCCTGAGTAAACTTCCCGCATAGTGGGGCGTCAGACGCCCCCTCAAACATTAAAATGTGAGCACTTTATCGGCTGACAGCGTCCATTGCGCCAGTTCCACAAGAGTACCGATTTCCACCCCATCAA > NZ_CP009273/1268519‑1268778 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |