Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,444,183	T→G	Q475P (CAG→CCG)	tynA ←	primary‑amine oxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,444,183	0	T	G	100.0%	92.0 / NA	26	Q475P (CAG→CCG)	tynA	primary‑amine oxidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (11/15);  total (11/15)

CGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACGTTCAAATACCGCGATAGCGCGAGGGATCTCCATCGGCACGCCAGTGTAGTCGGCGATGGTTTCATTAAGGAGCA  >  NZ_CP009273/1444049‑1444297                                                                                                                                       |                                                                                                                   cGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCggg                                                                                                                  <  2:165222/137‑1 (MQ=255)         gTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCt                                                                                                        <  1:487044/139‑1 (MQ=255)         gTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCt                                                                                                        <  1:477146/139‑1 (MQ=255)                                   gCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATaa                                                                              >  2:318645/1‑139 (MQ=255)                                    ccGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAAc                                                                             >  1:216153/1‑139 (MQ=255)                                    ccGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAAc                                                                             >  1:282033/1‑139 (MQ=255)                                          tCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACGTTCa                                                                        <  2:167236/138‑1 (MQ=255)                                                   aTCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACGTTCAAATACCGCGa                                                              <  1:120421/139‑1 (MQ=255)                                                         tCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACGTTCAAATACCGCGATAgcgc                                                        <  1:456213/139‑1 (MQ=255)                                                           aaaaaTGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACGTTCAAATACCGCGATAGCGCGa                                                      <  2:216153/139‑1 (MQ=255)                                                                                   tGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACGTTCAAATACCGCGATAGCGCGAGGGATCTCCATCGGCACGCCAgtg                              >  1:318882/1‑139 (MQ=255)                                                                                     tACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACGTTCAAATACCGCGATAGCGCGAGGGATCTCCATCGGCACGCCAgtg                              >  2:64104/1‑137 (MQ=255)                                                                                           tCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACGTTCAAATACCGCGATAGCGCGAGGGATCTCCATCGGCACGCCAGTGTAGCCGGc                      >  2:296220/1‑139 (MQ=255)                                                                                             cAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACGTTCAAATACCGCGATAGCGCGAGGGATCTCCATCGGCACGCCAGTGTAGTCGGCg                     <  1:318645/138‑1 (MQ=255)                                                                                               gcgcACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACGTTCAAATACCGCGATAGCGCGAGGGATCTCCAt                                           <  2:56306/114‑1 (MQ=255)                                                                                               gcgcACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACGTTCAAATACCGCGATAGCGCGAGGGATCTCCAt                                           >  1:56306/1‑114 (MQ=255)                                                                                                     cacTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACGTTCAAATACCGCGATAGCGCGAggg                                                   <  1:254543/100‑1 (MQ=255)                                                                                                     cacTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACGTTCAAATACCGCGATAGCGCGAggg                                                   >  2:254543/1‑100 (MQ=255)                                                                                                     cacTAACTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACGTTCAAATACCGCGATAGCGCGAGGGATCTCCATCGGCACGCCAGTGTAGTCGGCGATGGTTTCa            <  2:282033/139‑1 (MQ=255)                                                                                                          aCTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACGTTCAAATACCGCGATAGCGCGAGGGATCTCCATCGGCa                                      >  2:442961/1‑108 (MQ=255)                                                                                                          aCTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACGTTCAAATACCGCGATAGCGCGAGGGATCTCCATCGGCa                                      <  1:442961/108‑1 (MQ=255)                                                                                                          aCTCCCGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACGTTCAAATACCGCGATAGCGCGAGGGATCTCCATCGGCACGCCAGTGTAGTCGGCGATGGTTTCATTa         <  2:225626/137‑1 (MQ=255)                                                                                                               cGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACGTTCAAATACCGCGATAGCGCGAGGGATCTCCATCGGCACGCCAGTGTAGTCGGCGATGGTTTCATTAAGGAGCa  >  2:427726/1‑139 (MQ=255)                                                                                                               cGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACGTTCAAATACCGCGATAGCGCGAGGGATCTCCATCGGCACGCCAGTGTAGTCGGCGATGGTTTCATTAAGGAGCa  <  2:64268/139‑1 (MQ=255)                                                                                                               cGGCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACGTTCAAATACCGCGATAGCGCGAGGGATCTCCATCGGCACGCCAGTGTAGTCGGCGATGGTTTCATTAAGGAGCa  <  1:427726/139‑1 (MQ=255)                                                                                                                 gCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACGTTCAAATACCGCGATAGCGCGAGGGATCTCCATCGGCACGCCAGTGTAGTCGGCGATGGTTTCATTAAGGAGCa  <  2:218877/137‑1 (MQ=255)                                                                                                                 gCGTTCGGTACTGACGTTGGGCGGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACGTTCAAATACCGCGATAGCGCGAGGGATCTCCATCGGCACGCCAGTGTAGTCGGCGATGGTTTCATTAAGGAGCa  >  1:218877/1‑137 (MQ=255)                                                                                                                                   gggcgggcCCATTTCCTGATGCTTATACTCCGGCCCGGCAt                                                                                >  2:277915/1‑41 (MQ=255)                                                                                                                                   gggcgggcCCATTTCCTGATGCTTATACTCCGGCCCGGCAt                                                                                <  1:277915/41‑1 (MQ=255)                                                                                                                                       |                                                                                                                   CGATGCCCGTAGCACCGGCATCGATGCCAATAGTGCCGTTTTCATGGAAGATCCAGTCAAAAATGTAGTCATAGTTACCCACTGTACTGATCCAGCGCACCACTAACTCCCGGCGTTCGGTACTGACGTTGGGCTGGCCCATTTCCTGATGCTTATACTCCGGCCCGGCATAACGTTCAAATACCGCGATAGCGCGAGGGATCTCCATCGGCACGCCAGTGTAGTCGGCGATGGTTTCATTAAGGAGCA  >  NZ_CP009273/1444049‑1444297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑