Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,695,442	T→G	F77V (TTT→GTT)	malY →	bifunctional maltose regulon transcriptional repressor/cystathionine beta‑lyase MalY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,695,442	0	T	G	100.0%	172.3 / NA	44	F77V (TTT→GTT)	malY	bifunctional maltose regulon transcriptional repressor/cystathionine beta‑lyase MalY
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (20/24);  total (20/24)

TCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTAT  >  NZ_CP009273/1695311‑1695576                                                                                                                                    |                                                                                                                                       tCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCAc                                                                                                                                 >  2:45700/1‑139 (MQ=255)   aCGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCAcc                                                                                                                                <  1:256764/138‑1 (MQ=255)    cGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCAcc                                                                                                                                >  1:48789/1‑137 (MQ=255)    cGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCAcc                                                                                                                                >  2:380693/1‑137 (MQ=255)    cGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCAcc                                                                                                                                <  2:48789/137‑1 (MQ=255)                   aTTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCAt                                                                                                               <  1:450129/139‑1 (MQ=255)                       tGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGa                                                                                                            <  1:45700/138‑1 (MQ=255)                       tGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGAt                                                                                                           >  2:2052/1‑139 (MQ=255)                       tGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGAt                                                                                                           >  2:462196/1‑139 (MQ=255)                            cTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATtct                                                                                                        >  1:497020/1‑137 (MQ=255)                            cTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCa                                                                                                      <  1:310365/139‑1 (MQ=255)                               cccccTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGa                                                                                                    <  2:344393/138‑1 (MQ=255)                               cccccTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGa                                                                                                    <  1:79541/138‑1 (MQ=255)                                 cccTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACgg                                                                                                 <  1:46141/139‑1 (MQ=255)                                 cccTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACgg                                                                                                 <  2:170084/139‑1 (MQ=255)                                          aTCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATg                                                                                        >  2:388142/1‑139 (MQ=255)                                           tCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATgg                                                                                       <  2:185558/139‑1 (MQ=255)                                            cGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGc                                                                                      >  2:452937/1‑139 (MQ=255)                                             gAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGcc                                                                                     <  2:453911/139‑1 (MQ=255)                                              aGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGccc                                                                                    >  2:167619/1‑139 (MQ=255)                                                gcgcTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCtt                                                                                  >  1:113283/1‑139 (MQ=255)                                                  gcTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCt                                                                                <  1:33619/139‑1 (MQ=255)                                                   cTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTg                                                                               <  1:380693/139‑1 (MQ=255)                                                     gAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGt                                                                              >  2:365062/1‑138 (MQ=255)                                                           gcgcCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCta                                                                        >  2:417196/1‑138 (MQ=255)                                                             gcCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTatat                                                                     <  2:285462/139‑1 (MQ=255)                                                                      aCGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGa                                                            <  2:247114/139‑1 (MQ=255)                                                                        ggCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAAc                                                          <  2:113283/139‑1 (MQ=255)                                                                           gTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGa                                                       <  1:452937/139‑1 (MQ=255)                                                                                   cTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAg                                               <  1:462196/139‑1 (MQ=255)                                                                                    tACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTaca                                                                                                                      <  2:23398/67‑1 (MQ=255)                                                                                    tACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTaca                                                                                                                      >  1:23398/1‑67 (MQ=255)                                                                                    tACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGt                                              >  2:164911/1‑139 (MQ=255)                                                                                      cAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCtt                                                                                  >  2:261972/1‑101 (MQ=255)                                                                                      cAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCtt                                                                                  <  1:261972/101‑1 (MQ=255)                                                                                      cAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTgg                                            >  2:434576/1‑139 (MQ=255)                                                                                      cAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTgg                                            >  2:57138/1‑139 (MQ=255)                                                                                      cAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTgg                                            >  2:187602/1‑139 (MQ=255)                                                                                       aGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGt                                           <  1:167619/139‑1 (MQ=255)                                                                                                          aGTTTCTCGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCg                         <  2:281685/138‑1 (MQ=255)                                                                                                              tctcGCGGCTATTGCCCACTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCgtggt                     <  1:432075/138‑1 (MQ=255)                                                                                                                                cTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTc                                                                             <  2:280255/64‑1 (MQ=255)                                                                                                                                cTGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTc                                                                             >  1:280255/1‑64 (MQ=255)                                                                                                                                 tGGGTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGCGAAGGCGTGGTGATCCACACACCCGCCTAt  >  2:294618/1‑138 (MQ=255)                                                                                                                                    |                                                                                                                                       TCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTAT  >  NZ_CP009273/1695311‑1695576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑