Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,776,120	A→G	I157V (ATT→GTT)	ydiS →	FAD‑dependent oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,776,120	0	A	G	100.0%	148.5 / NA	40	I157V (ATT→GTT)	ydiS	FAD‑dependent oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/24);  total (16/24)

TGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGTTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGAT  >  NZ_CP009273/1775988‑1776245                                                                                                                                     |                                                                                                                              tGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGTTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTgg                                                                                                                         >  1:451408/1‑139 (MQ=255)        tGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGTTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATgg                                                                                                                  >  1:171899/1‑139 (MQ=255)           aaCAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGTTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGt                                                                                                               <  2:451408/139‑1 (MQ=255)                gCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGTTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACt                                                                                                          <  1:117583/139‑1 (MQ=255)                    aGCAGGCGGGCGCGCAGTTTATCCCAGGCGTTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGAt                                                                                                      >  1:13382/1‑139 (MQ=255)                        ggcgggcgCGCAGTTTATCCCAGGCGTTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGGGGTTCTGGCTGATGGCGTTAACTCGaacatt                                                                                                  >  2:316029/1‑134 (MQ=255)                              cgcgcAGTTTATCCCAGGCGTTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGtt                                                                                                                             <  1:73792/106‑1 (MQ=255)                              cgcgcAGTTTATCCCAGGCGTTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGtt                                                                                                                             >  2:73792/1‑106 (MQ=255)                                             aGGCGTTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGtt                                                                             >  1:346499/1‑139 (MQ=255)                                               gCGTTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGa                                                                                                                     >  2:360481/1‑97 (MQ=255)                                               gCGTTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGa                                                                                                                     <  1:360481/97‑1 (MQ=255)                                               gCGTTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGtt                                                                                                              <  1:293513/104‑1 (MQ=255)                                               gCGTTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGtt                                                                                                              >  2:293513/1‑104 (MQ=255)                                               gCGTTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTcc                                                                           >  2:262911/1‑139 (MQ=255)                                                            cgcgCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCg                                                              <  2:107414/139‑1 (MQ=255)                                                            cgcgCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCg                                                              <  1:119694/139‑1 (MQ=255)                                                                    ttCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTa                                                      <  1:93018/139‑1 (MQ=255)                                                                      cGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCg                                                                                                                >  2:397880/1‑79 (MQ=255)                                                                      cGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCg                                                                                                                <  1:397880/79‑1 (MQ=255)                                                                       gTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACg                                                    <  1:83176/138‑1 (MQ=255)                                                                          aaGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGt                                                <  2:13382/139‑1 (MQ=255)                                                                                    ggTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTa                                        >  2:9788/1‑137 (MQ=255)                                                                                        aCTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCg                                                              >  1:297875/1‑111 (MQ=255)                                                                                        aCTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCg                                                              <  2:297875/111‑1 (MQ=255)                                                                                        aCTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAgg                                  >  2:397657/1‑139 (MQ=255)                                                                                               tGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTgg                           >  1:309063/1‑139 (MQ=255)                                                                                                  aGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCt                        <  1:448143/139‑1 (MQ=255)                                                                                                  aGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCt                        <  2:87011/139‑1 (MQ=255)                                                                                                  aGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCt                        <  2:427501/139‑1 (MQ=255)                                                                                                  aGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCt                        <  1:9022/139‑1 (MQ=255)                                                                                                  aGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCt                        <  2:309063/139‑1 (MQ=255)                                                                                                  aGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCt                        <  1:114498/139‑1 (MQ=255)                                                                                                  aGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCt                        <  2:209127/139‑1 (MQ=255)                                                                                                  aGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCt                        <  2:171899/139‑1 (MQ=255)                                                                                                        gggATGATATTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCacac                   >  1:39690/1‑138 (MQ=255)                                                                                                              atatTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCg                                                              >  1:446017/1‑89 (MQ=255)                                                                                                              atatTCTCGAAGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCg                                                              <  2:446017/89‑1 (MQ=255)                                                                                                                        aGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGAt  <  1:131811/139‑1 (MQ=255)                                                                                                                        aGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGAt  <  2:111458/139‑1 (MQ=255)                                                                                                                        aGCGAATGTGGTGGTTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGAt  <  1:489881/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                              TGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGTTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTCTGGCTGATGGCGTTAACTCGATGCTTGGCCGCTCGCTGGGAATGGTTCCCGCTTCCGATCCGCATCATTACGCTGTTGGTGTTAAAGAGGTTATTGGCCTCACACCAGAACAGATCAACGAT  >  NZ_CP009273/1775988‑1776245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑