Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,806,054	T→C	S288S (AGT→AGC)	tcyP →	cystine/sulfocysteine:cation symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,806,054	0	T	C	100.0%	63.0 / NA	18	S288S (AGT→AGC)	tcyP	cystine/sulfocysteine:cation symporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/9);  total (9/9)

ATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTT  >  NZ_CP009273/1805923‑1806188                                                                                                                                    |                                                                                                                                       aTGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGc                                                                                                                                    <  2:84525/136‑1 (MQ=255)     ccAAAGTGGTTGTAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTa                                                                                                                             <  2:264615/139‑1 (MQ=255)     ccAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTa                                                                                                                             <  1:131458/139‑1 (MQ=255)             gTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGt                                                                                                                      <  1:199843/138‑1 (MQ=255)                                  gACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGt                                                                                                >  2:441765/1‑139 (MQ=255)                                                                gcgTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGt                                                                  <  2:293018/139‑1 (MQ=255)                                                                             gTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGaa                                                     >  1:134754/1‑139 (MQ=255)                                                                                tgctgATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGc                                                                                  >  1:345805/1‑107 (MQ=255)                                                                                tgctgATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGc                                                                                  <  2:345805/107‑1 (MQ=255)                                                                                  ctgATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAAtgtg                                                 >  2:40346/1‑138 (MQ=255)                                                                                            ttGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTa                                                                                                                             >  2:190532/1‑52 (MQ=255)                                                                                            ttGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTa                                                                                                                             <  1:190532/52‑1 (MQ=255)                                                                                                        gCATTCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTggg                          <  1:340737/139‑1 (MQ=255)                                                                                                            tCTGCTGGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCCGGGCGtt                      >  2:178980/1‑139 (MQ=255)                                                                                                              tgctgGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAAcgcg                                   >  1:62539/1‑124 (MQ=255)                                                                                                              tgctgGGCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAAcgcg                                   <  2:62539/124‑1 (MQ=255)                                                                                                                   ggCATTAATGGCGTGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAAt               >  2:281260/1‑139 (MQ=255)                                                                                                                                tGAGCCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGttt  >  1:193818/1‑139 (MQ=255)                                                                                                                                    |                                                                                                                                       ATGACCAAAGTGGTTGCAGGTTCTAACTTGCAAGACATCATCAAACTGGGAAGTTTCGTTGTCGCGTCCTACCTCGGTCTGCTGATTATGTTTGCAGTGCATGGCATTCTGCTGGGCATTAATGGCGTGAGTCCGCTGAAGTACTTCCGTAAGGTATGGCCTGTGCTGACGTTTGCCTTTACCAGCCGTTCCAGTGCTGCGTCTATCCCACTGAATGTGGAAGCACAAACGCGTCGTCTGGGCGTTCCTGAATCCATCGCCAGTTT  >  NZ_CP009273/1805923‑1806188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑