Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,819,028	T→C	E56G (GAA→GGA)	ves ←	environmental stress‑induced protein Ves

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,819,028	0	T	C	100.0%	80.4 / NA	22	E56G (GAA→GGA)	ves	environmental stress‑induced protein Ves
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/11);  total (11/11)

TGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTCGCCA  >  NZ_CP009273/1818893‑1819155                                                                                                                                        |                                                                                                                                tgccctgccGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTCCCa                                                                                                                              >  2:218768/1‑139 (MQ=255)        ccGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTCCCATGCCgg                                                                                                                        >  2:382930/1‑138 (MQ=255)                                 gTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTCCCATGCCGGGAAACAAAGAAAACTCCCCATTcgcc                                                                                              >  2:228091/1‑139 (MQ=255)                                     gCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTCCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCga                                                                                          <  2:44500/139‑1 (MQ=255)                                      cTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTCCCATGCCGGGAAACAAAGAAAACTCCCCATTcgcc                                                                                              >  2:312056/1‑134 (MQ=255)                                      cTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTCCCATGCCGGGAAACAAAGAAAACTCCCCATTcgcc                                                                                              <  1:312056/134‑1 (MQ=255)                                      cTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTCCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCgat                                                                                         <  1:187027/139‑1 (MQ=255)                                                    ggCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTCCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTgg                                                                           <  2:327302/139‑1 (MQ=255)                                                      cTGGAACGGTTTTAAGGTATGGTGAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTCCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGAGGCAATGCTGGCa                                                                         <  2:57436/139‑1 (MQ=255)                                                         gAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTCCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTGGCACg                                                                       <  1:305894/138‑1 (MQ=255)                                                               ttttAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTCCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTGGCACGCCAGTa                                                                 >  2:46723/1‑138 (MQ=255)                                                                         tGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTCCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTGGCACGCCAGTAAAAATCACGtt                                                      >  2:297887/1‑139 (MQ=255)                                                                         tGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTCCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTGGCACGCCAGTAAAAATCACGtt                                                      >  1:11856/1‑139 (MQ=255)                                                                         tGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTCCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTGGCACGCCAGTAAAAATCACGtt                                                      >  2:446421/1‑139 (MQ=255)                                                                                aGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTCCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCgg                                               <  1:382930/139‑1 (MQ=255)                                                                                     tCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTCCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTggg                                           >  2:306300/1‑138 (MQ=255)                                                                                                   gAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTCCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTcg                            <  1:228091/139‑1 (MQ=255)                                                                                                   gAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTCCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTcg                            <  1:218768/139‑1 (MQ=255)                                                                                                      cATCTCGCCGCCTTCCAGCAACGTCACTATCCTTCCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGaa                                         >  1:280945/1‑123 (MQ=255)                                                                                                      cATCTCGCCGCCTTCCAGCAACGTCACTATCCTTCCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGaa                                         <  2:280945/123‑1 (MQ=255)                                                                                                        tctcGCCGCCTTCCAGCAACGTCACTATCCTTCCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGt                       >  1:489254/1‑139 (MQ=255)                                                                                                                             tCACTATCCTTCCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTCGCca  <  1:226879/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                TGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCATGCCGGGAAACAAAGAAAACTCCCCATTCGCCGCGATGGATGCAATGCTGGCACGCCAGTAAAAATCACGTTTTGCCGGTGGGAACGTGCAAATTTCGCGCGTTTCACCGGCAGCATTTCGCCA  >  NZ_CP009273/1818893‑1819155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑