Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,865,194	A→C	N185H (AAC→CAC)	cdgI →	GGDEF domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,865,194	0	A	C	100.0%	87.3 / NA	24	N185H (AAC→CAC)	cdgI	GGDEF domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (16/8);  total (16/8)

TCCATAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTA  >  NZ_CP009273/1865066‑1865324                                                                                                                                 |                                                                                                                                   tCCATAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGa                                                                                                                          >  2:434568/1‑139 (MQ=255)     tAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGacaa                                                                                                                       >  2:340573/1‑138 (MQ=255)                 cGGCAGTACACTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGaaaaa                                                                                                           >  1:78331/1‑138 (MQ=255)                      gTACACTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACgg                                                                                                      >  1:306131/1‑138 (MQ=255)                       tACACTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACggg                                                                                                     >  1:62637/1‑138 (MQ=255)                       tACACTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACggg                                                                                                     >  2:265802/1‑138 (MQ=255)                       tACACTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACggg                                                                                                     >  1:475314/1‑138 (MQ=255)                        acacTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGaa                                                                                                   >  1:284299/1‑139 (MQ=255)                              aTCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACTTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGt                                                                                             >  2:182928/1‑139 (MQ=255)                              aTCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGt                                                                                             >  1:123892/1‑139 (MQ=255)                                        aGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCt                                                                                   >  1:152483/1‑139 (MQ=255)                                            gAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGAt                                                                                <  2:306131/138‑1 (MQ=255)                                             aaTGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATcc                                                                              <  2:475314/139‑1 (MQ=255)                                                    tttctattttctatATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCttttt                                                                        >  2:474201/1‑138 (MQ=255)                                                               tataTTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTcccc                                                            >  1:95611/1‑139 (MQ=255)                                                                                   ttttATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGc                                        <  2:284299/139‑1 (MQ=255)                                                                                   ttttATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGc                                        <  2:378571/139‑1 (MQ=255)                                                                                    tttATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAg                                         >  1:102672/1‑137 (MQ=255)                                                                                                    cGCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTa                       >  2:55713/1‑139 (MQ=255)                                                                                                     gCTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTAt                      <  2:152483/139‑1 (MQ=255)                                                                                                      cTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGc                                        <  2:293057/120‑1 (MQ=255)                                                                                                      cTGGCGCTCTTTTGTTATGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGc                                        >  1:293057/1‑120 (MQ=255)                                                                                                                       tGGAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGa     <  1:474201/138‑1 (MQ=255)                                                                                                                         gAAAAGACCACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTa  <  1:340573/139‑1 (MQ=255)                                                                                                                                 |                                                                                                                                   TCCATAAACCAATTAACGGCAGTACACTGATCCAGACAAAGTCGAATGATGTTTCTATTTTCTATATTTTCCGCCAACTCAGTTTTATTTGTTTAACCTCGCTGGCGCTCTTTTGTTATGGAAAAGACAACATCCTTGACAACAATAAGAAAAAAACGGGAATCCTGTTGCTGGCGCTGATCCCTTTTTTAGTTTTTCCCCTTCTGGCACACAATCTGAGCAGTTATAACGCTGACTATTCTTTGTATGTCGTCGATTA  >  NZ_CP009273/1865066‑1865324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑