Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,976,083	T→G	A313A (GCA→GCC)	araH ←	arabinose ABC transporter permease AraH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,976,083	0	T	G	100.0%	73.8 / NA	22	A313A (GCA→GCC)	araH	arabinose ABC transporter permease AraH
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (9/13);  total (9/13)

GGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATG  >  NZ_CP009273/1975948‑1976206                                                                                                                                        |                                                                                                                            gggTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGGTATGGTGGGGAAAAAAGCTAAAATGTTTCAGAAAAAAACATCAGACAGTGCGTTTTCCTTTTTTTTTTTTACGGTCGAAGATCACCCCGGcc                                                                                                                          >  1:153398/1‑139 (MQ=255)   gTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGGAAAAAAACAAAAATTTTGGAGAAAAAAAAAACAAAAAGTGGGTTTTGCTTTTTTTTTTTAACGGTCGAAAATCACCGCGGcccg                                                                                                                        >  2:364720/1‑137 (MQ=255)   gTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCGGCcag                                                                                                                        <  2:22502/139‑1 (MQ=255)              cAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCGGCCAGCAGGATTAAg                                                                                                              <  2:491924/138‑1 (MQ=255)                 cGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCGGCCAGCAGGATTAAGCcgc                                                                                                          <  2:87372/139‑1 (MQ=255)                 cGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCGGCCAGCAGGATTAAGCcgc                                                                                                          <  1:257653/139‑1 (MQ=255)                 cGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCGGCCAGCAGGATTAAGCcgc                                                                                                          <  2:331008/139‑1 (MQ=255)                 cGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCGGCCAGCAGGATTAAGCcgc                                                                                                          <  2:207057/139‑1 (MQ=255)                 cGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCGGCCAGCAGGATTAAGCcgc                                                                                                          <  2:167127/139‑1 (MQ=255)                                     tGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCCCTTTTTGCTTGTAACGGTCGAAGATCACCGCGGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGaa                                                                                       <  2:153398/138‑1 (MQ=255)                                        cGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCGGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGga                                                                                   <  1:10703/139‑1 (MQ=255)                                                               cGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCGGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGgag                                                                                  >  2:91444/1‑117 (MQ=255)                                                               cGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCGGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGgag                                                                                  <  1:91444/117‑1 (MQ=255)                                                                         ttgCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCGGCCAGCAGGATTAAGCcgc                                                                                                          <  1:376715/83‑1 (MQ=255)                                                                         ttgCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCGGCCAGCAGGATTAAGCcgc                                                                                                          >  2:376715/1‑83 (MQ=255)                                                                         ttgCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCGGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACg                                                  <  1:364720/139‑1 (MQ=255)                                                                                                tGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCGGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGc                           >  2:108165/1‑139 (MQ=255)                                                                                                tGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCGGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGc                           >  2:21561/1‑139 (MQ=255)                                                                                                tGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCGGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGc                           >  1:451922/1‑139 (MQ=255)                                                                                                tGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCGGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGc                           >  1:166285/1‑139 (MQ=255)                                                                                                                       cGGTCGAAGATCACCGCGGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGa    <  1:108165/139‑1 (MQ=255)                                                                                                                         gTCGAAGATCACCGCGGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATg  >  2:456187/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                            GGGTCGCCATTTGCAACGAAGAACAGCCAACCGTTATGGCGGTTGGCTATGGTGGGAAAAAACGCTAAATTGTTGCAGAAAAAAGCATCAGACAGTGCGTTTCGCTTTTTGCTTGTAACGGTCGAAGATCACCGCTGCCAGCAGGATTAAGCCGCGAACCACGTACTGCGCGAAAGGAGAAATATTAAGCAGGTTCATGGCGTTTTCCACGGTGCCTAAAATTAAGATACCCGCCACCACATATGAGATTTTTCCGATG  >  NZ_CP009273/1975948‑1976206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑