Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,000,655	A→G	E340G (GAA→GGA)	amyA →	alpha‑amylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,000,655	0	A	G	100.0%	103.8 / NA	30	E340G (GAA→GGA)	amyA	alpha‑amylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/15);  total (15/15)

CGACTACGACATGACGCAGATTTTCACGGGTACATTAGTGGAAGCCGATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCGGTATTCTATCCGGACCTCTACGGTGCGCATTACGAAGATGTCGGTGGTGACGGGCAAAC  >  NZ_CP009273/2000548‑2000787                                                                                                            |                                                                                                                                     cgACTACGACATGACGCAGATTTTCACGGGTACATTAGTGGAAGCCGATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGGAGCGCCGGTCGAACCGTGGTTTAAACCGCTg                                                                                                       >  1:108210/1‑139 (MQ=255)       cGACATGACGCAGATTTTCACGGGTACATTAGTGGAAGCCGATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGGAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTAt                                                                                                 >  2:22004/1‑139 (MQ=255)                                    tAGTGGAAGCCGATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGGAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGt                                                                    >  1:427748/1‑139 (MQ=255)                                      gTGGAAGCCGATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGGAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTc                                                                  <  2:108210/139‑1 (MQ=255)                                      gTGGAAGCCGATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGGAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTc                                                                  <  1:56185/139‑1 (MQ=255)                                       tGGAAGCCGATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGGAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCTTAATTTTGTTGCGGGAAAATGGCg                                                                     <  2:271446/135‑1 (MQ=255)                                       tGGAAGCCGATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGGAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCTTAATTTTGTTGCGGGAAAATGGCg                                                                     >  1:271446/1‑135 (MQ=255)                                       tGGAAGCCGATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGGAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTcc                                                                 >  2:350412/1‑139 (MQ=255)                                        ggAAGCCGATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGGAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCt                                                                >  1:182611/1‑139 (MQ=255)                                         gAAGCCGATCCTTTCCACGCCGTGACGCTCGATGCCAATCACGACACCCAACCGTTGCAAGCCCTCGGAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATACCTTAATTTTGTTGCGGGAAAATGGCGTTCCtt                                                               >  2:117451/1‑139 (MQ=255)                                           aGCCGATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGGAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCg                                                             <  2:422155/139‑1 (MQ=255)                                           aGCCGATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGGAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCg                                                             <  1:22004/139‑1 (MQ=255)                                            gCCGATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGGAGCGCCGGTCGAACCGTGGTTTAAACCg                                                                                                          <  1:232514/93‑1 (MQ=255)                                            gCCGATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGGAGCGCCGGTCGAACCGTGGTTTAAACCg                                                                                                          >  2:232514/1‑93 (MQ=255)                                                aTCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGGAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCGGTAtt                                                        >  1:15295/1‑139 (MQ=255)                                                aTCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGGAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCGGTAtt                                                        >  2:324364/1‑139 (MQ=255)                                                      tCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGGAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCGGTATTCTATcc                                                  >  2:266154/1‑139 (MQ=255)                                                      tCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGGAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCGGTATTCTATc                                                   <  1:70962/138‑1 (MQ=255)                                                        cACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGGAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCGGTATTCTATCCg                                                 <  2:182611/138‑1 (MQ=255)                                                                       gTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGGAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCGGTATTCTATCCGGACCTCTACGGTgcg                                  <  2:427748/138‑1 (MQ=255)                                                                         tGCCAATCACGACACCCAACCGTTGCAAGCCCTCGGAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCGGTATTCTATCCGGACCTCTACGGTGCGCAt                               >  1:10867/1‑139 (MQ=255)                                                                                cACGACACCCAACCGTTGCAAGCCCTCGGAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCGGTATTCTATCCGGACCTCTACGGTGCGCATTACg                           >  2:471740/1‑136 (MQ=255)                                                                                     cacCCAACCGTTGCAAGCCCTCGGAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCGGTATTCTATCCGGACCTCTACGGTGCGCATTACGAAGATGt                    >  2:255500/1‑138 (MQ=255)                                                                                     cacCCAACCGTTGCAAGCCCTCGGAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCGGTATTCTATCCGGACCTCTACGGTGCGCATTACGAAGATGt                    <  1:255500/138‑1 (MQ=255)                                                                                      acCCAACCGTTGCAAGCCCTCGGAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCGGTATTCTATCCGGACCTCTACGGTGCGCATTACGAAGATGTCg                  <  2:15295/139‑1 (MQ=255)                                                                                                      cccTCGGAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCGGTATTCTATCCGGACCTCTACGGTGCGCATTACGAAGATGTCGGTGGTGACGGGCAAAc  <  2:30575/139‑1 (MQ=255)                                                                                                      cccTCGGAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCGGTATTCTATCCGGACCTCTACGGTGCGCATTACGAAGATGTCGGTGGTGACGGGCAAAc  <  2:335861/139‑1 (MQ=255)                                                                                                      cccTCGGAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCGGTATTCTATCCGGACCTCTACGGTGCGCATTACGAAGATGTCGGTGGTGACGGGCAAAc  <  1:266154/139‑1 (MQ=255)                                                                                                         tCGGAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCGGTATTCTATCCGGACCTCTACGGTGCGCATTACGAAGATGTCGgtggtg            <  2:383860/126‑1 (MQ=255)                                                                                                         tCGGAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCGGTATTCTATCCGGACCTCTACGGTGCGCATTACGAAGATGTCGgtggtg            >  1:383860/1‑126 (MQ=255)                                                                                                            |                                                                                                                                     CGACTACGACATGACGCAGATTTTCACGGGTACATTAGTGGAAGCCGATCCTTTCCACGCCGTGACGCTCGTTGCCAATCACGACACCCAACCGTTGCAAGCCCTCGAAGCGCCGGTCGAACCGTGGTTTAAACCGCTGGCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCGGTATTCTATCCGGACCTCTACGGTGCGCATTACGAAGATGTCGGTGGTGACGGGCAAAC  >  NZ_CP009273/2000548‑2000787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑