| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,009,052 | A→G | D231G (GAC→GGC) | fliG → | flagellar motor switch protein FliG |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,009,052 | 0 | A | G | 100.0% | 84.2 / NA | 25 | D231G (GAC→GGC) | fliG | flagellar motor switch protein FliG |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (11/14); total (11/14) | |||||||||||
GAATCTCAAGCGCAGCAAAATGGGCGGCGTGAGAACGGCAGCCGAAATTATCAACCTGATGAAAACTCAGCAGGAAGAAGCCGTTATTACCGCCGTGCGTGAATTCGACGGCGAGCTGGCGCAGAAAATCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTC > NZ_CP009273/2008918‑2009182 | aaaTCTCAAGCGCAGCAAAATGGGCGGCGTGAGAACGGCAGCCGAAATTATCAACCTAATGAAAACACAACAGGAAGAATCCATTATTACCGCCGTGCGTGAATTCGACAGCGAGCTGGCGCAGAAAATCATCGGCgag < 1:153824/138‑1 (MQ=255) aaGCGCAGCAAAATGGGCGGCGTGAGAACGGCAGCCGAAATTATCAACCTGATGAAAACTCAGCAGGAAGAAGCCGTTATTACCGCCGTGCGTGAATTCGACGGCGAGCTGGCGCAGAAAATCATCGGCGAGATGt < 2:55275/136‑1 (MQ=255) aaGCGCAGCAAAATGGGCGGCGTGAGAACGGCAGCCGAAATTATCAACCTGATGAAAACTCAGCAGGAAGAAGCCGTTATTACCGCCGTGCGTGAATTCGACGGCGAGCTGGCGCAGAAAATCATCGGCGAGATGTTcc > 2:299548/1‑139 (MQ=255) agcaAAATGGGCGGCGTGAGAACGGCAGCCGAAATTATCAACCTGATGAAAACTCAGCAGGAAGAAGCCGTTATTACCGCCGTGCGTGAATTCGACGGCGAGCTGGCGCAGAAAATCATCGGCGAGATGTTCCTGTTc > 2:247471/1‑138 (MQ=255) gcaAAATGGGCGGCGTGAGAACGGCAGCCGAAATTATCAACCTGATGAAAACTCAGCAGGAAGAAGCCGTTATTACCGCCGTGCGTGAATTCGACGGCGAGCTGGCGCAGAAAATCATCGGCGAGATGTTCCTGTTCga < 1:366790/139‑1 (MQ=255) cgTGAGAACGGCAGCCGAAATTATCAACCTGATGAAAACTCAGCAGGAAGAAGCCGTTATTACCGCCGTGCGTGAATTCGACGGCGAGCTGGCGCAGAAAATCATCGGCGAGATGTTCCTGTTCga > 2:8606/1‑126 (MQ=255) cgTGAGAACGGCAGCCGAAATTATCAACCTGATGAAAACTCAGCAGGAAGAAGCCGTTATTACCGCCGTGCGTGAATTCGACGGCGAGCTGGCGCAGAAAATCATCGGCGAGATGTTCCTGTTCga < 1:8606/126‑1 (MQ=255) gTGAGAACGGCAGCCGAAATTATCAACCTGATGAAAACTCAGCAGTAAGAAGCCGTTATTACCGCCGTGCGTGAATTCGACGGCGAGCTGGCGCAGAAAATCATCGGCGAGATGTTCCTGTTCGAGAATCTGGTGGATg < 1:299548/139‑1 (MQ=255) gCCGAAATTATCAACCTGATGAAAACTCAGCAGGAAGAAGCCGTTATTACCGCCGTGCGTGAATTCGACGGCGAGCTGGCGCAGAAAATCATCGGCGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGATCg > 1:148675/1‑137 (MQ=255) aCCTGATGAAAACTCAGCAGGAAGAAGCCGTTATTACCGCCGTGCGTGAATTCGACGGCGAGCTGGCGCAGAAAATCATCGGCGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCt < 1:247471/139‑1 (MQ=255) atgaAAACTCAGCAGGAAGAAGCCGTTATTACCGCCGTGCGTGAATTCGACGGCGAGCTGGCGCAGAAAATCATCGGCGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTctgctg > 1:88892/1‑138 (MQ=255) aaCTCAGCAGGAAGAAGCCGTTATTACCGCCGTGCGTGAATTCGACGGCGAGCTGGCGCAGAAAATCATCGGCGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAgg > 2:21389/1‑137 (MQ=255) gaagaaGCCGTTATTACCGCCGTGCGTGAATTCGACGGCGAGCTGGCGCAGAAAATCATCGGCGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTcc > 1:482012/1‑138 (MQ=255) ttattaCCGCCGTGCGTGAATTCGACGGCGAGCTGGCGCAGAAAATCATCGGCGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGtt > 2:32958/1‑139 (MQ=255) cgtgcgtgAATTCGACGGCGAGCTGGCGCAGAAAATCATCGGCGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCt > 1:105727/1‑138 (MQ=255) tgcgtgAATTCGACGGCGAGCTGGCGCAGAAAATCATCGGCGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGaa < 1:366581/139‑1 (MQ=255) tgcgtgAATTCGACGGCGAGCTGGCGCAGAAAATCATCGGCGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGCTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGaa < 2:88892/139‑1 (MQ=255) cgtgAATTCGACGGCGAGCTGGCGCAGAAAATCATCGGCGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGaa < 2:78797/117‑1 (MQ=255) cgtgAATTCGACGGCGAGCTGGCGCAGAAAATCATCGGCGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGaa > 1:78797/1‑117 (MQ=255) aaTTCGACGGCGAGCTGGCGCAGAAAATCATCGGCGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGc < 2:444076/139‑1 (MQ=255) aaTTCGACGGCGAGCTGGCGCAGAAAATCATCGGCGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGc < 2:482012/139‑1 (MQ=255) aaTTCGACGGCGAGCTGGCGCAGAAAATCATCGGCGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGc < 2:15624/139‑1 (MQ=255) cGACGGCGAGCTGGCGCAGAAAATCATCGGCGAGATGTTCCTGTTCGAGAATCtggtgg > 2:45229/1‑59 (MQ=255) cGACGGCGAGCTGGCGCAGAAAATCATCGGCGAGATGTTCCGGTTCGAGAATCtggtgg < 1:45229/59‑1 (MQ=255) aaTCATCGGCGAGATGTTCCTGGTCGAGAATCTGGTGGACGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGATGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTc < 2:445799/139‑1 (MQ=255) | GAATCTCAAGCGCAGCAAAATGGGCGGCGTGAGAACGGCAGCCGAAATTATCAACCTGATGAAAACTCAGCAGGAAGAAGCCGTTATTACCGCCGTGCGTGAATTCGACGGCGAGCTGGCGCAGAAAATCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTC > NZ_CP009273/2008918‑2009182 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |