Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,065,124	T→G	G35G (GGT→GGG)	agn43 →	autotransporter adhesin Ag43

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,065,124	0	T	G	100.0%	39.8 / NA	12	G35G (GGT→GGG)	agn43	autotransporter adhesin Ag43
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (7/5);  total (7/5)

CTCAATCTAAGGAAAAGCTGATGAAACGACATCTGAATACCTGCTACAGGCTGGTATGGAATCACATGACGGGCGCTTTCGTGGTTGCCTCCGAACTGGCCCGCGCACGGGGTAAACGTGGCGGTGTGGCGGTTGCACTGTCTCTTGCCGCAGTCACGTCACTCCCGGTGCTGGCTGCTGACATCGTTGTGCACCCGGGAGAAACCGTGAACGGCGGAACACTGGCAAATCATGACAACCAGATTGTCTTCGGTACGAC  >  NZ_CP009273/2065000‑2065258                                                                                                                             |                                                                                                                                       cTCAATCTAAGGAAAAGCTGATGAAACGACATCTGAATACCTGCTACAGGCTGGTATGGAATCACATGACGGGCGCTTTCGTGGTTGCCTCCGAACTGGCCCGCGCACGGGGTAAACGTGGCGGGGTGGCGGTTGCACt                                                                                                                          <  2:28299/139‑1 (MQ=255)          aGGAAAAGCTGATGAAACGACATCTGAATACCTGCTACAGGCTGGTATGGAATCACATGACGGGCGCTTTCGTGGTTGCCTCCGAACTGGCCCGCGCACGGGGTAAACGTGGCGGGGTGGCGGTTGCACTGTCTCTTGc                                                                                                                 >  1:311033/1‑139 (MQ=255)                aGCTGATGAAACGACATCTGAATACCTGCTACAGGCTGGTATGGAATCACATGACGGGCGCTTTCGTGGTTGCCTCCGAACTGGCCCGCGCACGGGGTAAACGTGGCGGGGTGGCGGTTGCACTGTCTCTTGCCGCAgg                                                                                                           >  1:447773/1‑138 (MQ=255)                  cTGATGAAACGACATCTGAATACCTGCTACAGGCTGGTATGGAATCACATGACGGGCGCTTTCGTGGTTGCCTCCGAACTGGCCCGCGCACGGGGTAAACGTGGCGGGGTGGCGGTTGCACTGTCTCTTGCCGCAgtca                                                                                                         <  1:161656/139‑1 (MQ=255)                                           gCTACAGGCTGGTATGGAATCACATGACGGGCGCTTTCGTGGTTGCCTCCGAACTGGCCCGCGCACGGGGTAAACGTGGCGGGGTGGCGGTTGCACTGTCTCTTGCCGCAGTCACGTCACTCCCGGTGCTGGCTGCTGa                                                                                >  1:438698/1‑139 (MQ=255)                                                                acaTGACGGGCGCTTTCGTGGTTGCCTCCGAACTGGCCCGCGCACGGGGTAAACGTGGCGGGGTGGCGGTTGCACTTTCTCTTGCCGCAGTCACGTCACTCCCGGGGCTGGCTGCTGACATCGTTGTGCACCCggggg                                                            >  2:464483/1‑136 (MQ=255)                                                                acaTGACGGGCGCTTTCGTGGTTGCCTCCGAACTGGCCCGCGCACGGGGTAAACGTGGCGGGGTGGCGGTTGCACTGTCTCTTGCCGCAGTCACGTCACTCCCGGTGCTGGCTGCTGACATCGTTGTGCACCCGGgag                                                            <  2:311033/138‑1 (MQ=255)                                                                 caTGACGGGCGCTTTCGTGGTTGCCTCCGAACTGGCCCGCGCACGGGGTAAACGTGGCGGGGTGGCGGGTGCACTGGCTCTTGCCGCAGTCACGTCACTCCCGGGGCTGGCTGCTGACATCGTTGTGCACCCGGgaga                                                           >  2:183927/1‑138 (MQ=255)                                                                                              aaCTGGCCCGCGCACGGGGTAAACGTGGCGGGGGGGCGGTTGCACTGTCTCTTTCCGCAGTCACGTCACTCCCGGCGCTGGGTGCTGACATCGTTTTGCACCCGGGGGAAACCGCGGACGGCGGGACACTGGCCCATCa                             >  2:153459/1‑139 (MQ=255)                                                                                               aCTGGCCCGCGCACGGGGTAAACGTGGCGGGGTGGCGGTTGCACTGTCTCTTGCCGCAGTCACGTCACTCCCGGTGCTGGCTGCTGACATCGTTGTGCACCCGGGAGAAACCGTGAACGGCGGAACACTGGCAAATCAt                            <  1:146104/139‑1 (MQ=255)                                                                                                   gCCCGCGCACGGGGTAAACGTGGCGGGGTGGCGGTTGCACTGTCTCTTGCCGCAGTCACGTCACTCCCGGTGCTGGCTGCTGACATCGTTGTGCACCCGGgag                                                            >  1:80901/1‑103 (MQ=255)                                                                                                   gCCCGCGCACGGGGTAAACGTGGCGGGGTGGCGGTTGCACTGTCTCTTGCCGCAGTCACGTCACTCCCGGTGCTGGCTGCTGACATCGTTGTGCACCCGGgag                                                            <  2:80901/103‑1 (MQ=255)                                                                                                                         gcggggtggcggtTGCACTGTCTCTTGCCGCAGTCACGTCACTCCCGGGGCTGGCTGCTGACATCGTTGTGCACCCGGGAGAAACCGTGAACGGCGGAACACTGGCAAATCATGACAACCAGATTGTCTTCGGTACGAc  >  1:16649/1‑139 (MQ=255)                                                                                                                         gcggggtggcggtTGCACTGTCTCTTGCCGCAGTCACGGCACTCCCGGTGCTGGCTGCTGACATCGTTGTGCACCCGGGGGAAACCGGGAACGGCGGAAAACTGGCAAATCCTGACAACCAGGTTGTCTTCGGTACgga  >  2:257735/1‑137 (MQ=255)                                                                                                                             |                                                                                                                                       CTCAATCTAAGGAAAAGCTGATGAAACGACATCTGAATACCTGCTACAGGCTGGTATGGAATCACATGACGGGCGCTTTCGTGGTTGCCTCCGAACTGGCCCGCGCACGGGGTAAACGTGGCGGTGTGGCGGTTGCACTGTCTCTTGCCGCAGTCACGTCACTCCCGGTGCTGGCTGCTGACATCGTTGTGCACCCGGGAGAAACCGTGAACGGCGGAACACTGGCAAATCATGACAACCAGATTGTCTTCGGTACGAC  >  NZ_CP009273/2065000‑2065258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑