Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,071,153	G→C	G35A (GGC→GCC)	cbtA →	type IV toxin‑antitoxin system cytoskeleton‑binding toxin CbtA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,071,153	0	G	C	100.0%	55.9 / NA	16	G35A (GGC→GCC)	cbtA	type IV toxin‑antitoxin system cytoskeleton‑binding toxin CbtA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (9/7);  total (9/7)

AAAATCTTCAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGT  >  NZ_CP009273/2071038‑2071267                                                                                                                    |                                                                                                                   aaaaTCTTCAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGCCCTCACACTGAATGACACACCtt                                                                                             <  1:273909/139‑1 (MQ=255)               gAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGCCCTCACACTGAATGACACACCTTTTGCCGATGAACgt                                                                               >  2:396064/1‑139 (MQ=255)                         ccTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGCCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGag                                                                      <  2:70231/138‑1 (MQ=255)                             tATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGCCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGagcag                                                                   <  1:275795/137‑1 (MQ=255)                              aTTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGCCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGAtt                                                                         <  1:211626/130‑1 (MQ=255)                              aTTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGCCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGAtt                                                                         >  2:211626/1‑130 (MQ=255)                                 aCCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGCCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATAtt                                                             >  2:338367/1‑139 (MQ=255)                                       gCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGCCCTCACACTGAATGACACACCTTTTGCCGATGAACg                                                                                >  1:12998/1‑114 (MQ=255)                                       gCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGCCCTCACACTGAATGACACACCTTTTGCCGATGAACg                                                                                <  2:12998/114‑1 (MQ=255)                                       gCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGCCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGagg                                                         >  1:464276/1‑137 (MQ=255)                                                             tctcCTGTTGAAATCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGCCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCa                                              >  1:143046/1‑126 (MQ=255)                                                             tctcCTGTTGAAATCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGCCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCa                                              <  2:143046/126‑1 (MQ=255)                                                             tctcCTGTTGAAATCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGCCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACTGTGTGATGCgg                                 >  2:65989/1‑139 (MQ=255)                                                                        aaTCTGGCAAATACTGCTGTCCCGACTGCTGGACCAGCACTATGCCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACTGTGTGATGCGGTGAACTTtctc                      >  1:271536/1‑139 (MQ=255)                                                                                     ctgctgTCCCGACTGCTGGACCAGCACTATGCCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATAcg         <  2:153932/139‑1 (MQ=255)                                                                                            cccGACTGCTGGACCAGCACTATGCCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAACTACGCGCTGGt  >  1:473782/1‑139 (MQ=255)                                                                                                                    |                                                                                                                   AAAATCTTCAGTATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTCCCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTGAGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGT  >  NZ_CP009273/2071038‑2071267

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑