Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,141,824	T→C	S224P (TCA→CCA)	yegD →	molecular chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,141,824	0	T	C	100.0%	69.5 / NA	22	S224P (TCA→CCA)	yegD	molecular chaperone
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/15);  total (7/15)

GGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGGTGGTACGACTGACTGTTCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAAGCCAGCCTGCTGGGTCACAGTGGTTGCCGTATTGGCGGTAACGATCTGGATATCGCGCTGGCGTTTAAAAACCTGATGCCACTGC  >  NZ_CP009273/2141691‑2141956                                                                                                                                      |                                                                                                                                     ggcgAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGGTGGTACGACTGACTGTCCATtg                                                                                                                                 <  1:194668/139‑1 (MQ=255)         agacaggATTCAGGGCCGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGGTGGTACGACTGACTGTCCATTGCTGCTGAt                                                                                                                         <  1:389575/134‑1 (MQ=255)                                        cGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGGTGGTACGACTGACTGTCCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCg                                                                                           <  1:182878/138‑1 (MQ=255)                                        cGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGGTGGGACGACTGACTGTCCCTTTTTGCTGGTGGGGGCGGCGTGGGGGTCGCGTCTCGGTCGt                                                                                          >  1:121517/1‑139 (MQ=255)                                                     gctgggctggATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGGCGGGACGACTGACCGTCCCTTTTTTCTGGTGGGGGCGCCGGGGGGTTCGCGGCCCGCTTGTGAAGACAGCctcc                                                                             >  1:489504/1‑137 (MQ=255)                                                         ggctggATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGGTGGTACGACTGACTGTCCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAAGCCAGCctgctg                                                                           <  1:314834/137‑1 (MQ=255)                                                              gATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGGTGGTACGACTGACTGTCCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAAGCCAGCCTGCTGGGTcac                                                                     <  1:136386/138‑1 (MQ=255)                                                                        ccACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGGTGGTACGACTGACTGTCCATTGCTGCTGGTGGGGCCGCAGGGGCGGTCGCGTCTCGGTCGTGAAGCCAGCCTGCTGGGGCACAGGGGTTGCCg                                                          >  1:318802/1‑139 (MQ=255)                                                                                   gaagaaAAACGGGTGCTGGTGGTGGATATCGGCGGTGGTACGACTGACTGTCCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAAGCCAGCCTGCTGGGTCACAGTGGTTGCCGTATTGGCGGTa                                               <  1:45639/139‑1 (MQ=255)                                                                                   gaagaaAAACGGGTGCTGGTGGTGGATATCGGCGGTGGTACGACTGACTGTCCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAAGCCAGCCTGCTGGGTCACAGTGGTTGCCGTATTGGCGGTa                                               <  2:316150/139‑1 (MQ=255)                                                                                     agaaAAACGGGTGCTGGTGGTGGATATCGGCGGTGGGACGACTGACTGTCCATTTCTGCTGATGGGGGCGCCGTGGGGTTCGCGTCTCGGTCGTGAAGCCAGCCTGCTGGGGCACAGGGGTTGCCGGATTTGCGGGaa                                              >  1:302976/1‑138 (MQ=255)                                                                                     agaaAAACGGGTGCTGGTGGTGGATATCGGCGGTAGTACGGCCGGCTCTCCCTTGCCGCTGGTGGGGGCGCCGTGGGGTTCGCGTCTCCGACCTGAAGCCAGCCCGCTGGGGCCCAGAGGTTGGCGTATtggtgggga                                              >  1:150223/1‑135 (MQ=255)                                                                                          aaCTGGTGCTGGTGGTGGATATCGGCGGTGGTACGACTGACTGTCCATTGCTGCTGATGGGTCCGCAGTGGCGTTCGCGTCTCGATCGTGAAGCCAGCCTGCTGGGTCACAGTGGTTGCCGTATTGGCGGTAACGATCt                                        <  2:69796/139‑1 (MQ=255)                                                                                                        gtggATATCGGCGGTGGTACGACTGACTGTCCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAAGCCAGCCTGCTGGGTCACAGTGGTTGCCGTATTGGCGGTAACGATCTGGATATCGCGCTgg                          <  2:121517/139‑1 (MQ=255)                                                                                                         tggATATCGGCGGTGGTACGACTGACTGTCCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAAGCCAGCCTGCTGGGTCACAGTGGTTGCCGTATTGGCGGTAACGATCTGGATATCGCGCTGGc                         <  2:217944/139‑1 (MQ=255)                                                                                                               tCGGCGGTGGTACGACTGACTGTCCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAAGCCAGCCTGCTGGGTCACAGTGGTTGCCGTATTGGCGGTAACGATCTGGATATCGCGCTGGCGTTTaa                   <  2:409530/139‑1 (MQ=255)                                                                                                                     gtggtACGACTGACTGTCCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAAGCCAGCCTGCTGGGTCACAGTGGTTGCCGTATTGGCGGTAACGATCTGGATATCGCGCTGGCGTTTAAAAACCt             <  1:155709/139‑1 (MQ=255)                                                                                                                                 actgTCCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAAGCCAGCCTGCTGGGTCACAGTGGTTGCCGTATTGGCGGTAACGATCTGGGTATCGCGCTGGCGTTTAAAAACCTGATGCCActg   >  2:202489/1‑137 (MQ=255)                                                                                                                                 actgTCCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAAGCCAGCCTGCTGGGTCACAGTGGTTGCCGTATTGGCGGTAACGATCTGGATATCGCGCTGGCGTTTAAAAACCTGATGCCActg   <  2:97778/137‑1 (MQ=255)                                                                                                                                 actgTCCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAAGCCAGCCTGCTGGGTCACAGTGGTTGCCGTATTGGCGGTAACGATCTGGATATCGCGCTGGCGTTTAAAAACCTGATGCCActg   <  1:202489/137‑1 (MQ=255)                                                                                                                                  ctgTCCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAAGCCAGCCTGCTGGGTCACAGTGGTTGCCGTATTGGCGGTAACGATCTGGATATCGCGCTGGCGTTTAAAAACCTGATGCCActgc  <  2:394726/137‑1 (MQ=255)                                                                                                                                  ctgTCCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAAGCCAGCCTGCTGGGTCACAGTGGTTGCCGTATTGGCGGTAACGATCTGGATATCGCGCTGGCGTTTAAAAACCTGATGCCActgc  >  1:394726/1‑137 (MQ=255)                                                                                                                                      |                                                                                                                                     GGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGGTGGTACGACTGACTGTTCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAAGCCAGCCTGCTGGGTCACAGTGGTTGCCGTATTGGCGGTAACGATCTGGATATCGCGCTGGCGTTTAAAAACCTGATGCCACTGC  >  NZ_CP009273/2141691‑2141956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑