Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	75,122	G→A	A339T (GCA→ACA)	setA →	sugar efflux transporter SetA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	75,122	0	G	A	100.0%	104.1 / NA	27	A339T (GCA→ACA)	setA	sugar efflux transporter SetA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/12);  total (15/12)

CGGATTGATTTTCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGTCG  >  NZ_CP009273/74995‑75251                                                                                                                                |                                                                                                                                  cGGATTGATTTTCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTaccacc                                                                                                                        >  1:345487/1‑139 (MQ=255)  ggATTGATTTTCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCt                                                                                                                       >  2:102446/1‑139 (MQ=255)           ttCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACt                                                                                                               <  2:65223/138‑1 (MQ=255)           ttCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACt                                                                                                               <  2:345487/138‑1 (MQ=255)             cTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTaa                                                                                                             >  1:493993/1‑138 (MQ=255)                        gTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTAc                                                                                                 <  1:372916/139‑1 (MQ=255)                                ttGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTaa                                                                                         >  2:363126/1‑139 (MQ=255)                                        gCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTctggct                                                                                 >  2:308211/1‑139 (MQ=255)                                            cAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCg                                                                             <  1:262054/139‑1 (MQ=255)                                            cAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCg                                                                             <  1:363126/139‑1 (MQ=255)                                            cAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCg                                                                             >  1:107644/1‑139 (MQ=255)                                                  tttAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTAtt                                                                        <  1:102446/138‑1 (MQ=255)                                                          tGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGc                                                                >  1:326687/1‑138 (MQ=255)                                                            tATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTAc                                                                                                 <  1:289070/103‑1 (MQ=255)                                                            tATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTAc                                                                                                 >  2:289070/1‑103 (MQ=255)                                                                  tCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGcaca                                                       <  1:370906/139‑1 (MQ=255)                                                                      cATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGt                                                   >  2:230078/1‑139 (MQ=255)                                                                                ggTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACt                                         <  2:107644/139‑1 (MQ=255)                                                                                ggTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACt                                         >  1:425986/1‑139 (MQ=255)                                                                                     tGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCt                                    >  1:228743/1‑139 (MQ=255)                                                                                     tGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCt                                    >  2:384818/1‑139 (MQ=255)                                                                                      gggATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCt                                    <  2:63568/138‑1 (MQ=255)                                                                                      gggATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCt                                    <  1:230078/138‑1 (MQ=255)                                                                                                       ggATTTAATGCCTGGAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCg                  >  2:218353/1‑139 (MQ=255)                                                                                                                    ggAAGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCtgttg     >  2:167995/1‑139 (MQ=255)                                                                                                                      aaGAGCGGGGACAGCTACCACCTTATTTACTAACAGTATTACTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGTc   >  2:435941/1‑139 (MQ=255)                                                                                                                       agagCGGGGACAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGTCg  <  2:469295/139‑1 (MQ=255)                                                                                                                                |                                                                                                                                  CGGATTGATTTTCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGTCG  >  NZ_CP009273/74995‑75251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑