Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,247,045	C→T	A194T (GCA→ACA)	nupX ←	NupC/NupG family nucleoside CNT transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,247,045	0	C	T	100.0%	51.0 / NA	15	A194T (GCA→ACA)	nupX	NupC/NupG family nucleoside CNT transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/7);  total (8/7)

GGCAAACAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGCCCGAGGAAAA  >  NZ_CP009273/2246974‑2247176                                                                        |                                                                                                                                    ggCAAACAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGTGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCa                                                                  >  1:354162/1‑139 (MQ=255)   cAAACAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGTGGCGTAACCAATCATTGTCGa                                                                                                                <  1:300599/91‑1 (MQ=255)   cAAACAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGTGGCGTAACCAATCATTGTCGa                                                                                                                >  2:300599/1‑91 (MQ=255)   cAAACAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGTGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGc                                                                               <  1:138000/124‑1 (MQ=255)   cAAACAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGTGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGc                                                                               >  2:138000/1‑124 (MQ=255)   cAAACAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGTGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCAtt                                                                <  1:325267/139‑1 (MQ=255)             cccGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGTGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAAGCGCTGTAAATAATTCATTGCGATTCAGa                                                      <  2:466826/139‑1 (MQ=255)                gCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGTGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGAcga                                                   <  2:220155/139‑1 (MQ=255)                  cAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGTGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGAc                                                     <  1:145576/135‑1 (MQ=255)                  cAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGTGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGAc                                                     >  2:145576/1‑135 (MQ=255)                       aTCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGTGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATaa                                            <  1:166630/139‑1 (MQ=255)                                      gccgccAGCAGATATTCCACAGGCACGCCCAGTGTGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTg                             >  2:155395/1‑139 (MQ=255)                                          ccAGCAGATATTCCACAGGCACGCCCAGTGTGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCg                          >  1:216373/1‑138 (MQ=255)                                                         caGGCACGCCCAGTGTGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGccc          >  1:9159/1‑139 (MQ=255)                                                                 cccAGTGTGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGCCCGAGGaaaa  >  2:229749/1‑139 (MQ=255)                                                                        |                                                                                                                                    GGCAAACAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGCCCGAGGAAAA  >  NZ_CP009273/2246974‑2247176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑