Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 2,253,834 A→G I352I (ATT→ATC)  fruA ← PTS fructose transporter subunit IIBC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,253,8340AG100.0% 131.9 / NA 36I352I (ATT→ATC) fruAPTS fructose transporter subunit IIBC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/22);  total (14/22)

CCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGAATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATACCACCAATGAAGCCAGAACCGGTGCTGACCGC  >  NZ_CP009273/2253706‑2253965
                                                                                                                                |                                                                                                                                   
cccAGCAGAACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGa                                                                                                                           <  1:466563/139‑1 (MQ=255)
 ccAGCAGAACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGAt                                                                                                                          <  1:296041/139‑1 (MQ=255)
            gCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAgcg                                                                                                               <  2:38495/139‑1 (MQ=255)
             cATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAgcgc                                                                                                              >  1:357025/1‑139 (MQ=255)
                    aGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATa                                                                                                       <  2:168514/139‑1 (MQ=255)
                    aGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATa                                                                                                       <  1:452380/139‑1 (MQ=255)
                                cTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAg                                                                                                                 >  1:177483/1‑117 (MQ=255)
                                cTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAg                                                                                                                 <  2:459289/117‑1 (MQ=255)
                                cTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAg                                                                                                                 >  1:459289/1‑117 (MQ=255)
                                cTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAg                                                                                                                 <  2:177483/117‑1 (MQ=255)
                                cTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGt                                                                                           >  2:452249/1‑139 (MQ=255)
                                cTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGt                                                                                           >  2:336436/1‑139 (MQ=255)
                                            aGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTa                                                                               <  1:172344/139‑1 (MQ=255)
                                            aGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTa                                                                               <  1:336436/139‑1 (MQ=255)
                                                 gCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAg                                                                                                                 <  2:461853/100‑1 (MQ=255)
                                                 gCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGAACGGTTTCAg                                                                                                                 >  1:461853/1‑100 (MQ=255)
                                                  cccTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGAt                                                                          >  2:447172/1‑138 (MQ=255)
                                                        gagaATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTc                                                                   <  2:487960/139‑1 (MQ=255)
                                                                              ccGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGc                                             >  1:360664/1‑139 (MQ=255)
                                                                               cGATCAGGTAGATCATCGCCAGACCGACCACCAAACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGcc                                            <  2:172884/139‑1 (MQ=255)
                                                                                   cAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCAt                                                                                                        <  2:429434/75‑1 (MQ=255)
                                                                                   cAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCAt                                                                                                        >  1:429434/1‑75 (MQ=255)
                                                                                   cAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCg                                                                                 >  2:407877/1‑98 (MQ=255)
                                                                                   cAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCg                                                                                 <  1:407877/98‑1 (MQ=255)
                                                                                        aGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAgcg                                                                                                               <  1:258504/63‑1 (MQ=255)
                                                                                        aGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAgcg                                                                                                               >  2:258504/1‑63 (MQ=255)
                                                                                              tcGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCg                                          <  1:156829/126‑1 (MQ=255)
                                                                                              tcGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCg                                          >  2:156829/1‑126 (MQ=255)
                                                                                              tcGCCAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATaccac                              >  1:356578/1‑138 (MQ=255)
                                                                                                 ccAGACCGACCACCAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATACCACCAAt                          <  1:220742/139‑1 (MQ=255)
                                                                                                         accaccAGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATACCACCAATGAAGc                     >  2:487407/1‑136 (MQ=255)
                                                                                                            accaGACTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATACCACCAATGAAGCCAGAAc               <  2:344945/139‑1 (MQ=255)
                                                                                                                  cTGGAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATACCACCAATGAAGCCAGAACCGGTGc         <  1:447172/139‑1 (MQ=255)
                                                                                                                     gAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATACCACCAATGAAGCCAGAACCGGTGCTg       <  2:349332/138‑1 (MQ=255)
                                                                                                                     gAAATTAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATACCACCAATGAAGCCAGAACCGGTGCTg       <  1:487407/138‑1 (MQ=255)
                                                                                                                         ttAGCGGGATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATACCACCAATGAAGCCAGAACCGGTGCTGACCGc  <  2:111832/139‑1 (MQ=255)
                                                                                                                                |                                                                                                                                   
CCCAGCAGAACCGCATTCGCAGTCCCCATGGTCTGCAGCCAGTGAGTCAGCCCTTCGAGAATGCCAGCAACTGGTTTACCGATCAGGTAGATCATCGCCAGACCGACCACCAGACTGGAAATTAGCGGAATGATCAGGATCGGTTTCAGCGCCTCCATACTCTGTGGCAGTTTCAATTGCGTACTGATTAACTTCGCAATGTAACCAGCCAGGAAGCCCGCAATAATACCACCAATGAAGCCAGAACCGGTGCTGACCGC  >  NZ_CP009273/2253706‑2253965

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑