Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,378,215	A→G	D329D (GAT→GAC)	yfbK ←	VWA domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,378,215	0	A	G	100.0%	96.3 / NA	27	D329D (GAT→GAC)	yfbK	VWA domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/12);  total (15/12)

AGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCCTTCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTA  >  NZ_CP009273/2378079‑2378335                                                                                                                                         |                                                                                                                         aGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGGTc                                                                                                                        >  1:220294/1‑139 (MQ=255)  gCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGGTCg                                                                                                                       >  1:300815/1‑139 (MQ=255)    tGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGGTCGt                                                                                                                      >  1:456696/1‑138 (MQ=255)      tAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGGTCGTCaa                                                                                                                   >  2:210754/1‑139 (MQ=255)        gTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGGTCGTCAATg                                                                                                                 >  1:233068/1‑139 (MQ=255)                         cGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGGTCGTCAATGCCAACGTTAAAGTCAcc                                                                                                >  2:419738/1‑139 (MQ=255)                             aaTTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGGTCGTCAATGCCAACGTTAAAGTCACCGTCa                                                                                            <  2:456696/139‑1 (MQ=255)                                  gCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGGTCGTCAATGCCAACGTTAAAGTCACCGTCAGTgg                                                                                        <  2:439807/138‑1 (MQ=255)                                                tCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGGTCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGAt                                                                         >  2:73731/1‑139 (MQ=255)                                                tCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGGTCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGAt                                                                         >  1:100759/1‑139 (MQ=255)                                                tCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGGTCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGAt                                                                         >  2:189550/1‑139 (MQ=255)                                                      tAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGGTCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATg                                                                    <  2:220294/138‑1 (MQ=255)                                                                      cGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGGTCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAAccc                                                   >  1:307103/1‑139 (MQ=255)                                                                               tCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGGTCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCCTTCGTCGc                                          <  1:212441/139‑1 (MQ=255)                                                                                 gACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGGTCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCCTTCGTCGcc                                         <  2:100759/138‑1 (MQ=255)                                                                                 gACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGGTCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCCTTCGTCGCct                                        <  2:212351/139‑1 (MQ=255)                                                                                      agTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGGTCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCCTTCGTCGCCTGCTGa                                   >  1:384165/1‑139 (MQ=255)                                                                                          acacCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGGTCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCCTTCGTCGCCTGCTGATAAg                               <  1:419738/139‑1 (MQ=255)                                                                                           cacCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGGTCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCCTTCGTCGCCTGCTGATAAGc                              <  1:73731/139‑1 (MQ=255)                                                                                           cacCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGGTCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCCTTCGTCGCCTGCTGATAAGc                              <  1:189550/139‑1 (MQ=255)                                                                                                gACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGGTCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCCTTCGTCGCCTGCTGATAAGCCAGtt                         >  2:301506/1‑139 (MQ=255)                                                                                                                     aTTGATTCAATCGATTTTGGGTCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCCTTCGTCGCCTGCTGATAAGCCAGTTCCAGc                    >  2:115554/1‑123 (MQ=255)                                                                                                                     aTTGATTCAATCGATTTTGGGTCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCCTTCGTCGCCTGCTGATAAGCCAGTTCCAGc                    <  1:115554/123‑1 (MQ=255)                                                                                                                     aTTGATTCAATCGATTTTGGGTCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCCTTCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCa              >  1:380340/1‑129 (MQ=255)                                                                                                                     aTTGATTCAATCGATTTTGGGTCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCCTTCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCa              <  2:380340/129‑1 (MQ=255)                                                                                                                     aTTGATTCAATCGATTTTGGGTCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCCTTCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTgg    >  1:137114/1‑139 (MQ=255)                                                                                                                        gATTCAATCGATTTTGGGTCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCCTTCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTa  <  1:176530/138‑1 (MQ=255)                                                                                                                                         |                                                                                                                         AGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCATTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCCTTCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTA  >  NZ_CP009273/2378079‑2378335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑