Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 97,990 A→C T247P (ACC→CCC)  murC → UDP‑N‑acetylmuramate‑‑L‑alanine ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP00927397,9900AC100.0% 111.5 / NA 31T247P (ACC→CCC) murCUDP‑N‑acetylmuramate‑‑L‑alanine ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (17/14);  total (17/14)

ACCTACCAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGTCACCCTGAATGCG  >  NZ_CP009273/97855‑98115
                                                                                                                                       |                                                                                                                             
acctaccAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCa                                                                                                                            >  1:362091/1‑139 (MQ=255)
          gCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACgg                                                                                                                  >  1:213863/1‑139 (MQ=255)
             aCTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCtt                                                                                                               >  1:414630/1‑139 (MQ=255)
                        ttAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATg                                                                                                    >  2:256015/1‑139 (MQ=255)
                               aGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGAcgt                                                                                             <  2:362091/139‑1 (MQ=255)
                               aGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGAcgt                                                                                             <  1:311270/139‑1 (MQ=255)
                                 aCTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGAcgtgc                                                                                           <  1:296996/139‑1 (MQ=255)
                                               tCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTAt                                                                             >  1:413501/1‑139 (MQ=255)
                                                        ccTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGAtt                                                                    >  1:8472/1‑139 (MQ=255)
                                                                  tACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATca                                                                           >  1:297002/1‑122 (MQ=255)
                                                                  tACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATca                                                                           <  2:297002/122‑1 (MQ=255)
                                                                    cGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAggg                                                         <  2:414630/138‑1 (MQ=255)
                                                                    cGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAggg                                                         <  1:424450/138‑1 (MQ=255)
                                                                    cGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAggg                                                         <  1:435844/138‑1 (MQ=255)
                                                                       tCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCAc                                                     >  1:202128/1‑139 (MQ=255)
                                                                         gTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACtt                                                   >  2:203397/1‑139 (MQ=255)
                                                                                        ttGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCa                                    <  2:202128/139‑1 (MQ=255)
                                                                                         tgatgaTCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGccc                                                                                                                             >  1:20278/1‑49 (MQ=255)
                                                                                         tgatgaTCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGccc                                                                                                                             <  2:20278/49‑1 (MQ=255)
                                                                                         tgatgaTCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCa                                                                                                                            >  2:59628/1‑50 (MQ=255)
                                                                                         tgatgaTCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCa                                                                                                                            <  1:59628/50‑1 (MQ=255)
                                                                                                ccGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCAc                                                                                                                           <  2:127162/44‑1 (MQ=255)
                                                                                                ccGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCAc                                                                                                                           >  1:127162/1‑44 (MQ=255)
                                                                                                ccGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACa                               >  1:398632/1‑136 (MQ=255)
                                                                                                ccGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACa                               >  2:38901/1‑136 (MQ=255)
                                                                                                   gTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGc                         >  2:386496/1‑139 (MQ=255)
                                                                                                   gTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGc                         >  1:121265/1‑139 (MQ=255)
                                                                                                         cgcgAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATgc                   >  1:46351/1‑139 (MQ=255)
                                                                                                            gAATTGTTACCGCTAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGcgcg                <  1:401501/139‑1 (MQ=255)
                                                                                                            gAATTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGcgcg                <  2:398632/139‑1 (MQ=255)
                                                                                                             aaTTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGt               <  1:270557/139‑1 (MQ=255)
                                                                                                             aaTTGTTACCGCGAGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGt               <  1:203397/139‑1 (MQ=255)
                                                                                                                          aGTGGGGCGTCAGCCCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGTCACCCTGAATgcg  >  2:471230/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                             
ACCTACCAGGGCGACTTTGAGAATTTAAAACAGACTTTTATTAATTTTCTGCACAACCTGCCGTTTTACGGTCGTGCGGTGATGTGTGTTGATGATCCGGTGATCCGCGAATTGTTACCGCGAGTGGGGCGTCAGACCACGACTTACGGCTTCAGCGAAGATGCCGACGTGCGTGTAGAAGATTATCAGCAGATTGGCCCGCAGGGGCACTTTACGCTGCTGCGCCAGGACAAAGAGCCGATGCGCGTCACCCTGAATGCG  >  NZ_CP009273/97855‑98115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑