Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,547,539	A→G	S335P (TCC→CCC)	eutR ←	HTH‑type transcriptional regulator EutR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,547,539	0	A	G	100.0%	103.0 / NA	29	S335P (TCC→CCC)	eutR	HTH‑type transcriptional regulator EutR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/14);  total (15/14)

ATATGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTATTAAGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAA  >  NZ_CP009273/2547408‑2547667                                                                                                                                    |                                                                                                                                 atatGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTATTAAGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAgc                                                                                                                           >  2:5063/1‑139 (MQ=255)    tGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTATTAAGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAgctgc                                                                                                                        >  2:480926/1‑139 (MQ=255)          tCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTATTAAGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTaa                                                                                                                  >  2:382774/1‑139 (MQ=255)              ccAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTATTAAGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCg                                                                                                              >  1:449897/1‑139 (MQ=255)                            cccAGAAGCGGCGTTAAGTGAGTTTATTAAGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGccc                                                                                                 >  1:32425/1‑138 (MQ=255)                                      gcgTTAAGTGAGTTTATTAAGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAg                                                                                       >  1:161553/1‑138 (MQ=255)                                      gcgTTAAGTGAGTTTATTAAGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAg                                                                                       >  1:89140/1‑138 (MQ=255)                                            aGTGAGTTTATTAAGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATccc                                                                                 <  1:96035/138‑1 (MQ=255)                                            aGTGAGTTTATTAAGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATccc                                                                                 <  2:161553/138‑1 (MQ=255)                                             gTGAGTTTATTAAGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCa                                                                               <  1:5063/139‑1 (MQ=255)                                             gTGAGTTTATTAAGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCa                                                                               <  2:57515/139‑1 (MQ=255)                                             gTGAGTTTATTAAGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCa                                                                               <  1:370050/139‑1 (MQ=255)                                                     attaAGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATgg                                                                       >  2:145818/1‑139 (MQ=255)                                                               gggATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCttt                                                             <  1:480926/139‑1 (MQ=255)                                                               gggATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCttt                                                             <  1:243271/139‑1 (MQ=255)                                                                       gtgtAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCa                                                     <  1:145818/139‑1 (MQ=255)                                                                             cTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACttt                                               >  2:187807/1‑139 (MQ=255)                                                                                ccTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTg                                              <  2:210291/137‑1 (MQ=255)                                                                                  tCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGAc                                          >  1:465376/1‑139 (MQ=255)                                                                                              ccGCATCCGCTGATTCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTAt                               <  2:194512/138‑1 (MQ=255)                                                                                               cGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTAt                               >  2:470470/1‑137 (MQ=255)                                                                                                             cAGCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACg               <  2:449897/139‑1 (MQ=255)                                                                                                               gCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTg                                              >  1:73847/1‑106 (MQ=255)                                                                                                               gCGTCAGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTg                                              <  2:73847/106‑1 (MQ=255)                                                                                                                    aGTGACGGCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGtt          >  1:296894/1‑137 (MQ=255)                                                                                                                          ggCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACg               >  1:469880/1‑126 (MQ=255)                                                                                                                          ggCTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACg               <  2:469880/126‑1 (MQ=255)                                                                                                                            cTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGaa  <  1:434194/137‑1 (MQ=255)                                                                                                                            cTTCTCGGGAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGaa  >  2:434194/1‑137 (MQ=255)                                                                                                                                    |                                                                                                                                 ATATGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTATTAAGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACGGCTTCTCGGAAAACAGCTGCTGGTAATCCGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAA  >  NZ_CP009273/2547408‑2547667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑