Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,561,055	A→G	S138P (TCC→CCC)	eutH ←	ethanolamine utilization protein EutH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,561,055	0	A	G	100.0%	104.4 / NA	29	S138P (TCC→CCC)	eutH	ethanolamine utilization protein EutH
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/11);  total (18/11)

CGGAGTACATAGCAACCAGACCACCAGCGATACAACCAATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGA  >  NZ_CP009273/2560929‑2561193                                                                                                                               |                                                                                                                                           cGGAGTACATAGCAACCAGACCACCAGCGATACAACCAATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGGAAACACAATCGt                                                                                                                                >  2:19321/1‑139 (MQ=255)  ggAGTACATAGCAACCAGACCACCAGCGATACAACCAATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGGAAACACAATCGtt                                                                                                                               <  1:19321/139‑1 (MQ=255)           aGCAACCAGACCACCAGCGATACAACCAATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGGAAACACAATCGTTGGCCCcat                                                                                                                       >  1:381001/1‑138 (MQ=255)             cAACCAGACCACCAGCGATACAACCAATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGGAAACACAATCGTTGGCCCcatcat                                                                                                                    >  1:498384/1‑139 (MQ=255)                ccAGACCACCAGCGATACAACCAATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGGAAACACAATCGTTGGCCCCATCATCGa                                                                                                                 >  1:239739/1‑139 (MQ=255)                ccAGACCACCAGCGATACAACCAATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGGAAACACAATCGTTGGCCCCATCATCGa                                                                                                                 >  2:196999/1‑139 (MQ=255)                   gACCACCAGCGATACAACCAATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGGAAACACAATCGTTGGCCCCATCATCGAccc                                                                                                              >  1:267964/1‑139 (MQ=255)                    accaccAGCGATACAACCAATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCACCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGGAAACACAATCGTTGGACCCATCATCGAccc                                                                                                              >  1:147129/1‑138 (MQ=255)                          aGCGATACAACCAATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCACCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGGAAACACAATCGTTGGCCCCATCATCGACCCGAGAAt                                                                                                        >  2:173572/1‑138 (MQ=255)                              aTACAACCAATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGGAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAAcc                                                                                                   <  2:443142/139‑1 (MQ=255)                              aTACAACCAATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGGAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAAcc                                                                                                   <  2:267964/139‑1 (MQ=255)                              aTACAACCAATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGGAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAAcc                                                                                                   <  2:150583/139‑1 (MQ=255)                                 cAACCAATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGGAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCa                                                                                                 <  2:498384/138‑1 (MQ=255)                                 cAACCAATCGGAATGGTCACAAGGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGGAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCa                                                                                                 <  2:417552/138‑1 (MQ=255)                                      aaTCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGGAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGta                                                                                            >  1:248990/1‑138 (MQ=255)                                       aTCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGGAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGta                                                                                            >  2:390723/1‑137 (MQ=255)                                                gTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGGAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCgg                                                                                 >  2:310853/1‑139 (MQ=255)                                                           cGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGGAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTcgccg                                                                      >  1:206887/1‑139 (MQ=255)                                                           cGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGGAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTcgccg                                                                      >  1:290251/1‑139 (MQ=255)                                                           cGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGGAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTcgccg                                                                      >  1:229353/1‑139 (MQ=255)                                                                        aGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGGAAACACAATCGtt                                                                                                                               >  1:371865/1‑69 (MQ=255)                                                                        aGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGGAAACACAATCGtt                                                                                                                               <  2:371865/69‑1 (MQ=255)                                                                                       cgGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGGAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAgc                                          <  1:355162/139‑1 (MQ=255)                                                                                       cgGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGGAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAgc                                          >  2:271906/1‑139 (MQ=255)                                                                                                ggTTCGATAATGCCGAGCGCCACCGGAATGGGAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCatat                                 <  1:481013/139‑1 (MQ=255)                                                                                                            ccGAGCGCCACCGGAATGGGAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGc                          >  1:134406/1‑134 (MQ=255)                                                                                                            ccGAGCGCCACCGGAATGGGAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGc                          <  2:134406/134‑1 (MQ=255)                                                                                                            ccGAGCGCCACCGGAATGGGAAACACAATCGTTGGCCCCACCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCcagca                     <  2:473232/139‑1 (MQ=255)                                                                                                                    cACCGGAATGGGAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCg             >  1:78706/1‑139 (MQ=255)                                                                                                                               aaaaCCCAGTCGTTGGCCCCACCTTCGACCCGAGAATTAACCCAGAGTATACCCACGCGGTTACTTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGa  <  2:290251/139‑1 (MQ=255)                                                                                                                               |                                                                                                                                           CGGAGTACATAGCAACCAGACCACCAGCGATACAACCAATCGGAATGGTCACAATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGA  >  NZ_CP009273/2560929‑2561193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑