Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,926,085	A→C	G96G (GGT→GGG)	fucO ←	lactaldehyde reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,926,085	0	A	C	100.0%	182.9 / NA	50	G96G (GGT→GGG)	fucO	lactaldehyde reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (25/25);  total (25/25)

TGGTAGGAATTGCCAGAATCGGTACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCACCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAATTGTTGGGTTGGGCACTACGCCGTCGTAAATCGCCCATGCCAGCCCTGCAGCATCCATCTTATC  >  NZ_CP009273/2925955‑2926219                                                                                                                                   |                                                                                                                                       tgGTAGGAATTGCCAGAATCGGTACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAAt                                                                                                                                <  1:17060/139‑1 (MQ=255)  ggTAGGAATTGCCAGAATCGGTACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCaa                                                                                                                                 >  2:122216/1‑137 (MQ=255)      ggAATTGCCAGAATCGGTACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCg                                                                                                                            <  2:308380/138‑1 (MQ=255)           tGCCAGAATCGGTACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAgg                                                                                                                      >  1:95966/1‑139 (MQ=255)           tGCCAGAATCGGTACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAgg                                                                                                                      >  1:415634/1‑139 (MQ=255)           tGCCAGAATCGGTACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAgg                                                                                                                      >  2:241926/1‑139 (MQ=255)                       tACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGcgccg                                                                                                          <  2:95966/139‑1 (MQ=255)                       tACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGcgccg                                                                                                          <  1:452640/139‑1 (MQ=255)                       tACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGcgccg                                                                                                          >  2:228704/1‑139 (MQ=255)                       tACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGcgccg                                                                                                          <  1:361279/139‑1 (MQ=255)                          acTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTa                                                                                                       <  1:241926/139‑1 (MQ=255)                              ggTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTAtt                                                                                                     <  1:55537/137‑1 (MQ=255)                              ggTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTAtt                                                                                                     <  1:324095/137‑1 (MQ=255)                              ggTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTAtt                                                                                                     <  2:415634/137‑1 (MQ=255)                                        tCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACAcc                                                                                         >  1:360788/1‑139 (MQ=255)                                                      tCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTga                                                                           >  1:374052/1‑139 (MQ=255)                                                      tCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTga                                                                           >  2:477218/1‑139 (MQ=255)                                                       ccAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTgac                                                                          >  1:421312/1‑139 (MQ=255)                                                            cTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAg                                                                     <  2:303540/139‑1 (MQ=255)                                                               cGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGt                                                                    <  1:99502/137‑1 (MQ=255)                                                                  acaTCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAAttg                                                               <  2:360788/139‑1 (MQ=255)                                                                  acaTCGGCAAACTCCGGGTGGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAAttg                                                               <  1:207840/139‑1 (MQ=255)                                                                    aTCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAAttgtt                                                             >  2:95256/1‑139 (MQ=255)                                                                         cAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAATTgttgggtt                                                        <  2:365471/139‑1 (MQ=255)                                                                         cAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAATTgttgggtt                                                        <  1:122216/139‑1 (MQ=255)                                                                                   gtttttGCTGATAATGCCAATCCCTTTACAAGTCTCCTGTGGGGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAATTGTTGGGTTGGGCACTACg                                              <  1:422658/139‑1 (MQ=255)                                                                                       ttgCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGc                                                                                     >  1:173722/1‑96 (MQ=255)                                                                                       ttgCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGc                                                                                     <  2:173722/96‑1 (MQ=255)                                                                                       ttgCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAATTGTTGGGTTGGGCACTACGCcgt                                          >  1:273790/1‑139 (MQ=255)                                                                                       ttgCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAATTGTTGGGTTGGGCACTACGCcgt                                          >  2:330285/1‑139 (MQ=255)                                                                                        tgCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAATTGTTGGGTTGGGCACTACGCcgtc                                         >  2:199848/1‑139 (MQ=255)                                                                                          cTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAATTGTTGGGTTGGGCACTACGCcgtcg                                        >  1:189025/1‑138 (MQ=255)                                                                                                 tGCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAATTGTTGGGTTGGGCACTACGCCGTCGTAAATCGc                                >  1:468940/1‑139 (MQ=255)                                                                                                  gCCAATCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAATTGTTGGGTTGGGCACTACGCCGTCGTAAATCGcc                               >  1:277974/1‑139 (MQ=255)                                                                                                       tCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAATTGTTGGGTTGGGCACTACGCCGTCGTAAATCGCCCATGc                          <  2:421312/139‑1 (MQ=255)                                                                                                       tCGCTTTACAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAATTGTTGGGTTGGGCACTACGCCGTCGTAAATCGCCCATGc                          <  2:374052/139‑1 (MQ=255)                                                                                                               cAAGTATCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAATTGTTGGGTTGGGCACTACGCCGTCGTAAATCGCCCATGCCAGCCCTg                  <  1:95256/139‑1 (MQ=255)                                                                                                                     tCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTAtt                                                                                                     >  2:345519/1‑50 (MQ=255)                                                                                                                     tCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTAtt                                                                                                     <  1:345519/50‑1 (MQ=255)                                                                                                                     tCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGt                                                                    >  2:382646/1‑83 (MQ=255)                                                                                                                     tCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGt                                                                    <  1:382646/83‑1 (MQ=255)                                                                                                                     tCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAATTGTTGGGTTGGGCACTACGCCGTCGTAAATCGCCCATGCCAGCCCTgcagca             <  1:199848/138‑1 (MQ=255)                                                                                                                     tCCTGTGGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAATTGTTGGGTTGGGCACTACGCCGTCGTAAATCGCCCATGCCAGCCCTGCAGcat            >  1:73269/1‑139 (MQ=255)                                                                                                                         gtgGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAATTGTTGGGTTGGGCACTACGCCGTCGTAAATCGCCCATGCCAGCCCTGCAGcatccat        >  1:320084/1‑139 (MQ=255)                                                                                                                          tgGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGt                                                                    >  2:119236/1‑78 (MQ=255)                                                                                                                          tgGAGAACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGt                                                                    <  1:119236/78‑1 (MQ=255)                                                                                                                            gagaACCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAATTGTTGGGTTGGGCACTACGCCGTCGTAAATCGCCCATGCCAGCCCTGCAGCATCCATCt      >  1:213440/1‑138 (MQ=255)                                                                                                                               aaCCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTgacg                                                                         >  1:378317/1‑68 (MQ=255)                                                                                                                               aaCCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTgacg                                                                         <  2:378317/68‑1 (MQ=255)                                                                                                                               aaCCCCCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAATTGTTGGGTTGGGCACTACGCCGTCGTAAATCGCCCATGCCAGCCCTGCAGCATCCATCTTATc  >  2:268038/1‑139 (MQ=255)                                                                                                                                   |                                                                                                                                       TGGTAGGAATTGCCAGAATCGGTACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGATAATGCCAATCGCTTTACAAGTATCCTGTGGAGAACCACCACCAATAGCGATCAGGTAATCCGCGCCGCTATTCTGGAATACACCGAGCCCTTCTTTGACGACAGTAATTGTTGGGTTGGGCACTACGCCGTCGTAAATCGCCCATGCCAGCCCTGCAGCATCCATCTTATC  >  NZ_CP009273/2925955‑2926219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑