Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,960,176	G→A	R540W (CGG→TGG)	ptsP ←	phosphoenolpyruvate‑‑protein phosphotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,960,176	0	G	A	100.0%	60.0 / NA	16	R540W (CGG→TGG)	ptsP	phosphoenolpyruvate‑‑protein phosphotransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/7);  total (9/7)

ACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATCCCACGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGAC  >  NZ_CP009273/2960042‑2960311                                                                                                                                       |                                                                                                                                        aCCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCacac                                                                                                                                      <  2:4813/138‑1 (MQ=255) aCCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCacac                                                                                                                                      <  2:304681/138‑1 (MQ=255)    gATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCACACCTgg                                                                                                                                  <  2:236879/139‑1 (MQ=255)               cGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCACACCTGGATCaag                                                                                                                            >  2:401114/1‑134 (MQ=255)                      aCGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCACACCTGGATCAAGAAGATCTCCGGc                                                                                                                <  1:58145/139‑1 (MQ=255)                                                          aaCTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCACACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATCCCACGCCAACCCAgg                                                                            <  2:226523/139‑1 (MQ=255)                                                           aCTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCACACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATCCCACGCCAACCCAGGc                                                                           <  1:177295/139‑1 (MQ=255)                                                                aTCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCACACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATCCCACGCCAACCCAGGCATGGa                                                                      >  2:250616/1‑139 (MQ=255)                                                                      gCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCACACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATcc                                                                                          <  1:461076/113‑1 (MQ=255)                                                                      gCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCACACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATcc                                                                                          >  2:461076/1‑113 (MQ=255)                                                                           tgACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCACACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATCCCACGCCAACCCAGGCATGGATTCTCTTCGCt                                                           >  2:254092/1‑139 (MQ=255)                                                                                  cGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCACACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGc                                                                                                <  2:260578/95‑1 (MQ=255)                                                                                  cGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCACACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGc                                                                                                >  1:260578/1‑95 (MQ=255)                                                                                                     ttGCCCGTAGCGGCATTAGCACGCAGCATCGCCCACACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATCCCACGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTAt                                 <  1:254092/139‑1 (MQ=255)                                                                                                       gCCCGTAGCGGCATTAGCACGCAGCATCGCCCACACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATCCCACGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCt                               >  1:449/1‑139 (MQ=255)                                                                                                       gCCCGTAGCGGCATTAGCACGCAGCATCGCCCACACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATCCCACGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCt                               >  2:184466/1‑139 (MQ=255)                                                                                                                            cagcaTCGCCCACACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATCCCACGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACg           >  1:294335/1‑138 (MQ=255)                                                                                                                                    cccACACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATCCCACGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGAc  >  1:260641/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                        ACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATCCCACGCCAACCCAGGCATGGATTCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGAC  >  NZ_CP009273/2960042‑2960311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑