Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,027,998	T→C	E179G (GAA→GGA)	lysS ←	lysine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,027,998	0	T	C	100.0%	74.4 / NA	23	E179G (GAA→GGA)	lysS	lysine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/10);  total (13/10)

TAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGT  >  NZ_CP009273/3027864‑3028129                                                                                                                                       |                                                                                                                                    tAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATCCATc                                                                                                                                 >  1:139990/1‑139 (MQ=255) tAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATCCATc                                                                                                                                 >  1:402478/1‑139 (MQ=255)        aCGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATCCATCGTTg                                                                                                                             >  2:354633/1‑136 (MQ=255)         cgcgCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATCCATCGTTGgaga                                                                                                                         <  2:454282/139‑1 (MQ=255)               gCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATCCATCGTTGgagatgagat                                                                                                                   <  2:159726/139‑1 (MQ=255)                      cGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATCCATCGTTGGAGATGAGATCGAGATa                                                                                                            <  1:456887/139‑1 (MQ=255)                                      catcatCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATCCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAgcg                                                                                             <  1:474175/138‑1 (MQ=255)                                          atcGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATCCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCtt                                                                                        >  1:227291/1‑139 (MQ=255)                                           tcGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATCCATCGTTGGAGATGAGATCGAGATa                                                                                                            <  1:227075/118‑1 (MQ=255)                                           tcGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATCCATCGTTGGAGATGAGATCGAGATa                                                                                                            >  2:227075/1‑118 (MQ=255)                                               cGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATCCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGa                                                                                   <  1:361204/139‑1 (MQ=255)                                                                      cgTTTACCATGGACTGGCGAATTCCAGGGAAGATCTGCGAGCGCACTTTAAAGGTGGTGCGGGGTCCATCGTTGGAGATGAGATCCAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATc                                                            <  2:402478/138‑1 (MQ=255)                                                                           aCCATGAACTGGCGAATACCAGAGAGGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATCCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGc                                                        <  2:401210/138‑1 (MQ=255)                                                                                                gagagGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATCCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGt                                    >  1:298659/1‑137 (MQ=255)                                                                                                            gAGCGCACTTTAAAGGTGTTGCGGGATCCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACt                      <  2:227291/139‑1 (MQ=255)                                                                                                                              ttGCGGGATCGATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGa      >  2:275713/1‑137 (MQ=255)                                                                                                                              ttGCGGGATCCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGa                                                  >  1:93652/1‑93 (MQ=255)                                                                                                                              ttGCGGGATCCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGa                                                  <  2:93652/93‑1 (MQ=255)                                                                                                                              ttGCGGGATCCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGa      >  1:313151/1‑137 (MQ=255)                                                                                                                              ttGCGGGATCCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGa      >  1:28916/1‑137 (MQ=255)                                                                                                                              ttGCGGGATCCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGa      >  2:468778/1‑137 (MQ=255)                                                                                                                              ttGCGGGATCCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGa      >  2:58359/1‑137 (MQ=255)                                                                                                                                gCGGGATCCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGGGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGAGGATAGACAGt  >  2:71400/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                    TAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGT  >  NZ_CP009273/3027864‑3028129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑