Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,104,251	T→G	T79P (ACC→CCC)	yghD ←	GspM family type II secretion system protein YghD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,104,251	0	T	G	100.0%	136.4 / NA	38	T79P (ACC→CCC)	yghD	GspM family type II secretion system protein YghD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (17/21);  total (17/21)

CCACGATCGGCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTAACATGCCCTTTTCACGCG  >  NZ_CP009273/3104115‑3104385                                                                                                                                         |                                                                                                                                       ccACGATCGGCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGgggt                                                                                                                                      >  2:55687/1‑139 (MQ=255)     gATCGGCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCAt                                                                                                                                  <  1:55687/139‑1 (MQ=255)          gCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGcca                                                                                                                             >  1:332272/1‑139 (MQ=255)          gCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGcca                                                                                                                             >  1:347252/1‑139 (MQ=255)          gCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGcca                                                                                                                             >  1:34732/1‑139 (MQ=255)              tCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGccacc                                                                                                                           <  2:471603/137‑1 (MQ=255)                cGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAgc                                                                                                                        <  2:90382/138‑1 (MQ=255)                      atgaCCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCtgctgc                                                                                                                  >  2:290921/1‑138 (MQ=255)                      atgaCCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGAGGTTCATCGCCACCAGCtgctgc                                                                                                                  <  2:354345/138‑1 (MQ=255)                         accaccGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAata                                                                                                              <  2:34732/139‑1 (MQ=255)                         accaccGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAata                                                                                                              <  2:23574/139‑1 (MQ=255)                            accGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAata                                                                                                              >  1:362129/1‑136 (MQ=255)                            accGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAata                                                                                                              <  2:362129/136‑1 (MQ=255)                             ccGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGt                                                                                                          >  1:265088/1‑139 (MQ=255)                              cGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGtc                                                                                                         <  2:5339/139‑1 (MQ=255)                                aGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGtctc                                                                                                       >  2:93477/1‑139 (MQ=255)                                aGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGtctc                                                                                                       >  1:9/1‑139 (MQ=255)                                     gcgGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGtt                                                                                                  >  2:484734/1‑139 (MQ=255)                                                 tCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGtt                                                                                      <  1:395469/139‑1 (MQ=255)                                                      gatgaCCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCt                                                                                                     <  1:307104/119‑1 (MQ=255)                                                      gatgaCCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCt                                                                                                     >  2:307104/1‑119 (MQ=255)                                                           cccGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACgg                                                                            <  1:290921/139‑1 (MQ=255)                                                                    ccTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGa                                                                    <  1:412824/138‑1 (MQ=255)                                                                     cTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATg                                                                  <  2:463046/139‑1 (MQ=255)                                                                     cTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATg                                                                  <  1:173018/139‑1 (MQ=255)                                                                                ttgttgTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAata                                                       >  2:258038/1‑139 (MQ=255)                                                                                   ttgtGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATc                                                     <  2:9/138‑1 (MQ=255)                                                                                                  gcgcTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGaa                                     <  2:229573/139‑1 (MQ=255)                                                                                                        cAATAATATCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAAcacca                                <  1:269013/138‑1 (MQ=255)                                                                                                                tCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAata                                                                                                              >  2:258850/1‑52 (MQ=255)                                                                                                                tCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAata                                                                                                              <  1:258850/52‑1 (MQ=255)                                                                                                                tCCGGCGCGGCGTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGc                         >  2:419510/1‑137 (MQ=255)                                                                                                                   ggcgcggcgTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCa                       >  2:215302/1‑136 (MQ=255)                                                                                                                   ggcgcggcgTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCa                       <  1:215302/136‑1 (MQ=255)                                                                                                                     cgcggcgTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAAt                                                                                           >  1:63611/1‑66 (MQ=255)                                                                                                                     cgcggcgTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAAt                                                                                           <  2:63611/66‑1 (MQ=255)                                                                                                                     cgcggcgTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCa                       >  2:29181/1‑134 (MQ=255)                                                                                                                     cgcggcgTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCa                       <  1:29181/134‑1 (MQ=255)                                                                                                                        ggcgTTCTTCAGTCGGGGGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTAACATg               >  2:327483/1‑139 (MQ=255)                                                                                                                                      ggggGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTAACATGCCCTTTTCAcgcg  >  2:390790/1‑138 (MQ=255)                                                                                                                                         |                                                                                                                                       CCACGATCGGCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTAACATGCCCTTTTCACGCG  >  NZ_CP009273/3104115‑3104385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑