Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,246,701	A→G	I291I (ATT→ATC)	yhaJ ←	DNA‑binding transcriptional regulator YhaJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,246,701	0	A	G	100.0%	117.5 / NA	32	I291I (ATT→ATC)	yhaJ	DNA‑binding transcriptional regulator YhaJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/16);  total (16/16)

AGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCGATCTCGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATA  >  NZ_CP009273/3246585‑3246833                                                                                                                     |                                                                                                                                     agcaATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGAtt                                                                                                                >  1:12078/1‑139 (MQ=255) agcaATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGAtt                                                                                                                >  1:458024/1‑139 (MQ=255)  gcaATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGAttt                                                                                                               <  1:281731/139‑1 (MQ=255)     aTCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGc                                                                                                            <  2:476327/139‑1 (MQ=255)     aTCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGc                                                                                                            <  1:437619/139‑1 (MQ=255)        gTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCtt                                                                                                          <  2:122288/138‑1 (MQ=255)            cGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTccccc                                                                                                     <  1:399750/139‑1 (MQ=255)             ggAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCa                                                                                                    <  2:12078/139‑1 (MQ=255)                       cGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCac                                                                                           <  2:175827/138‑1 (MQ=255)                         aaGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCacgac                                                                                        >  2:427574/1‑139 (MQ=255)                                gTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGc                                                                                 <  2:203019/139‑1 (MQ=255)                                                           atatTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCGATCTCGCTGGTCGa                                                      >  1:181669/1‑139 (MQ=255)                                                           atatTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCGATCTCGCTGGTCGa                                                      >  2:206531/1‑139 (MQ=255)                                                                      cTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGc                                                                                 <  1:176274/101‑1 (MQ=255)                                                                      cTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGc                                                                                 >  2:176274/1‑101 (MQ=255)                                                                      cTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCGATCTCGCTGGTCGATTCCGGGCTga                                           >  1:80675/1‑139 (MQ=255)                                                                        ttttATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCGATCTCGCTGGTCGATTCCGGGCTga                                           >  1:369971/1‑137 (MQ=255)                                                                        ttttATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCGATCTCGCTGGTCGATTCCGGGCTga                                           <  2:369971/137‑1 (MQ=255)                                                                                 cAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGc                                                                                 <  1:52941/90‑1 (MQ=255)                                                                                 cAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGc                                                                                 >  2:52941/1‑90 (MQ=255)                                                                                       taaataTTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCGATCTCGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCt                          >  1:185408/1‑139 (MQ=255)                                                                                          atatTATTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCGATCTCGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTcc                       >  2:461605/1‑139 (MQ=255)                                                                                           tattatTTTCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCGATCTCGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCg                      <  2:80675/139‑1 (MQ=255)                                                                                                ttttCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGc                                                                                                            >  1:404375/1‑48 (MQ=255)                                                                                                ttttCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGc                                                                                                            <  2:404375/48‑1 (MQ=255)                                                                                                  ttCCGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCGATCTCGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATc               >  2:313288/1‑139 (MQ=255)                                                                                                     cGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCGATCTCGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCttt            >  1:328600/1‑139 (MQ=255)                                                                                                     cGTTAAAAAGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCGATCTCGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCttt            >  1:66328/1‑139 (MQ=255)                                                                                                          aaaaGTTTGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCGATCTCGCTGTTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGa       <  1:1819/139‑1 (MQ=255)                                                                                                               tttGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCGATCTCGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCa    >  1:488510/1‑137 (MQ=255)                                                                                                               tttGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCGATCTCGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATa  <  2:185408/139‑1 (MQ=255)                                                                                                               tttGGGGATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCGATCTCGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATa  <  2:181669/139‑1 (MQ=255)                                                                                                                     |                                                                                                                                     AGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCGATCTCGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATA  >  NZ_CP009273/3246585‑3246833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑