Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,058,644	Δ1 bp	coding (483/1404 nt)	yihO ←	MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,058,644	0	A	.	100.0%	128.8 / NA	30	coding (483/1404 nt)	yihO	MFS transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (11/19);  total (11/19)

GCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGCACCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAG  >  NZ_CP009273/4058512‑4058763                                                                                                                                     |                                                                                                                        gCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCAccc                                                                                                                   <  2:272878/138‑1 (MQ=255)    caGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGc                                                                                                               >  1:279940/1‑139 (MQ=255)       catcatAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCgg                                                                                                             >  1:30301/1‑138 (MQ=255)                      gccgccAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTgcg                                                                                             <  2:279940/139‑1 (MQ=255)                            aaTGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCt                                                                                       <  2:394112/139‑1 (MQ=255)                                        aaGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGtttt                                                                           >  2:229732/1‑139 (MQ=255)                                                 acaGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTg                                                                  <  1:89430/139‑1 (MQ=255)                                                   aGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGcc                                                                <  1:229732/139‑1 (MQ=255)                                                               cGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCa                                                    >  2:69489/1‑139 (MQ=255)                                                               cGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCa                                                    >  2:420076/1‑139 (MQ=255)                                                               cGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCa                                                    >  2:410287/1‑139 (MQ=255)                                                                     aGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCcgcg                                                                                                    <  2:560623/85‑1 (MQ=255)                                                                     aGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCcgcg                                                                                                    >  1:560623/1‑85 (MQ=255)                                                                      gTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCggg                                                             <  2:220667/123‑1 (MQ=255)                                                                      gTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCggg                                                             >  1:220667/1‑123 (MQ=255)                                                                        cAGAAAAAAGCGACGTCAACGGAATAAACGCCACGGTACAAATCAGTAGCCCTATGGTTGC‑CCACCCGGGCGGTAAGCCGCGAGCTGCGCGCGCTCAGTCGGGTTTTTGGTAATTGCCGGGGTCATCGCACCAGACGAg                                           <  2:296629/139‑1 (MQ=255)                                                                         aGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAg                                           <  1:95451/138‑1 (MQ=255)                                                                                  cGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTtcatca                                 <  1:127205/139‑1 (MQ=255)                                                                                  cGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTtcatca                                 <  1:510400/139‑1 (MQ=255)                                                                                  cGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTtcatca                                 <  2:30301/139‑1 (MQ=255)                                                                                                   aCGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCg                                                      <  1:503673/101‑1 (MQ=255)                                                                                                   aCGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCg                                                      >  2:503673/1‑101 (MQ=255)                                                                                                    cGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCaa               <  1:470743/139‑1 (MQ=255)                                                                                                    cGCCACGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCaa               <  2:51886/139‑1 (MQ=255)                                                                                                         cGGTACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAAcatc          <  1:441098/139‑1 (MQ=255)                                                                                                            tACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCg                                                      >  1:431009/1‑92 (MQ=255)                                                                                                            tACAAATCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCg                                                      <  2:431009/92‑1 (MQ=255)                                                                                                                 aTCAATAGCCCTATGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAg  <  1:69489/139‑1 (MQ=255)                                                                                                                             aTGGTTGC‑CCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCgg                                                              <  2:220806/67‑1 (MQ=255)                                                                                                                             aTGGTTGC‑CCACCCTGGCGGTAAGCAGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCgg                                                              >  1:220806/1‑67 (MQ=255)                                                                                                                                     |                                                                                                                        GCACAGCATCATAAAAATAAAGCCGCCAATGGAGAACATAAGTGCCGCACAGGCATAACCGACGGTTGAGTCAGAAAAAAGCGACTGCAACGGAATAAACGCCACGGTACAAATCAATAGCCCTATGGTTGCACCACCCTGGCGGTAAGCCGCGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAG  >  NZ_CP009273/4058512‑4058763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑