Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	467,406	Δ1 bp	coding (1315/1782 nt)	mdlB →	SmdB family multidrug efflux ABC transporter permease/ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	467,406	0	C	.	100.0%	81.0 / NA	19	coding (1315/1782 nt)	mdlB	SmdB family multidrug efflux ABC transporter permease/ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (7/12);  total (7/12)

CTTGATGGTCGTCCATTAAGTTCGCTAAGTCACAGCGCGCTGCGCCAGGGCGTGGCAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGGCGGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAATCTC  >  NZ_CP009273/467289‑467522                                                                                                                      |                                                                                                                     cTTGATGGTCGTCCATTAAGTTCGCTAAGTCACAGCGCGCTGCGCCAGGGCGTGGCAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGG‑GGGATATCTCCGAAGAACGCGt                                                                                                <  1:331541/139‑1 (MQ=255)      tGGTCGTCCATTAAGTTCGCTAAGTCACAGCGCGCTGCGCCAGGGCGTGGCAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGG‑GGGATATCTCCGAAGAACGCGTCTGGc                                                                                           <  1:141928/139‑1 (MQ=255)        gtcgtcCATTAAGTTCGCTAAGTCACAGCGCGCTGCGCCAGGGCGTGGCAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGG‑GGGATATCTCCGAAGAACGCGTCTGGCAg                                                                                         <  2:478568/139‑1 (MQ=255)                       cGCTAAGTCACAGCGCGCTGCGCCAGGGCGTGGCAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGG‑GGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTg                                                                          >  2:292830/1‑139 (MQ=255)                                       gcTGCGCCAGGGCGTGGCAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGG‑GGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTgg                                                          <  1:550013/139‑1 (MQ=255)                                           cgcCAGGGCGTGGCAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGG‑GGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGcgcg                                                      <  1:145368/139‑1 (MQ=255)                                                        cAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGG‑GGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGAc                                         <  2:443080/139‑1 (MQ=255)                                                                   caAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGG‑GGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTacac                              <  2:352712/139‑1 (MQ=255)                                                                                tgCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGG‑GGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACACGCCGCTGGGCGAg                 <  2:322120/139‑1 (MQ=255)                                                                                 gCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGG‑GGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAg                                             <  1:355722/110‑1 (MQ=255)                                                                                 gCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGG‑GGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAg                                             >  2:355722/1‑110 (MQ=255)                                                                                 gCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGG‑GGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACAcgcc                           >  1:465202/1‑128 (MQ=255)                                                                                 gCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGG‑GGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACAcgcc                           <  2:465202/128‑1 (MQ=255)                                                                                      cGGATACCTTCCTCGCCAACGTGACGCTGGGG‑GGGATATCTCCGAAGAACGCGTCTGGCAg                                                                                         >  1:290377/1‑61 (MQ=255)                                                                                      cGGATACCTTCCTCGCCAACGTGACGCTGGGG‑GGGATATCTCCGAAGAACGCGTCTGGCAg                                                                                         <  2:290377/61‑1 (MQ=255)                                                                                         aTACCTTCCTCGCCAACGTGACGCTGGGG‑GGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACACGCCGCTGGGCGAGCAGGGGaat        <  1:292830/139‑1 (MQ=255)                                                                                          tACCTTCCTCGCCAACGTGACGCTGGGG‑GGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACACGCCGCTGGGCGAGCAGGGGaat        >  1:364977/1‑138 (MQ=255)                                                                                               tCCTCGCCAACGTGACGCTGGGG‑GGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAAtctc  >  2:487688/1‑139 (MQ=255)                                                                                               tCCTCGCCAACGTGACGCTGGGG‑GGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAAtctc  >  2:110765/1‑139 (MQ=255)                                                                                                                      |                                                                                                                     CTTGATGGTCGTCCATTAAGTTCGCTAAGTCACAGCGCGCTGCGCCAGGGCGTGGCAATGGTGCAGCAAGATCCGGTGGTGCTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGGCGGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTTACACGCCGCTGGGCGAGCAGGGGAATAATCTC  >  NZ_CP009273/467289‑467522

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑